Spontaneous Cytotoxic-Activity as a Test of Human Lymphocyte Function

Matthews, N.; Maclaurin, B. P.

doi:10.1016/s0140-6736(75)91607-4

Cited by 6 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In these studies, we have used the mouse mastocytoma cell line, P815, and the human CML line, K562, as the targets in assay systems for the detection and quantitation of the ability of human lymphocytes to mediate spontaneous cytotoxicity. These assays are referred to as the xenogeneic and allogeneic SLMC assays respectively, and reflect the cytotoxic activity of human non-T-lymphocytes (Pross and Jondal, 1975a, b;Jondal and Pross, 1975;Peter et al, 19756;Matthews and MacLaurin, 1975;de Vries et al, 1974;Kiuchi and Takasugi, 1975). This activity may be mediated by way of a non-immunoglobulin, lymphotoxin-like substance which requires the presence of effector cells (" K " cells?)…”

Section: Discussionmentioning

confidence: 99%

Spontaneous human lymphocyte‐mediated cytotoxicity againts tumour target cells. I. The effect of malignant disease

Pross

Baines

1976

Intl Journal of Cancer

238

View full text Add to dashboard Cite

Spontaneous human lymphocyte-mediated cytotoxicity (SLMC) against tumour-cell targets was examined in a series of patients with localized or malignant disease, both treated and untreated, and patients with untreated chronic lymphocytic leukemia (CLL). The level of SLMC was assessed by means of two previously established assay systems; the xenogeneic assay involving the mouse mastocytoma line P815, and the allogeneic assay in which the human chronic myelogenous leukemia-derived line, K562, was used. The assay systems involve the use of Ficoll-Isopaque-separated, iron-plus-magnetism-purified lymphocytes in an overnight 51chromium release assay, and reflect the cytotoxic ability of human non-T, complement receptor-, Fc receptor-positive lymphocytes. In the present paper, lymphocytes from all normal donors tested showed significant activity in the SLMC assay, with some variation from day to day. This variation was markedly reduced when different normal donors were tested on the same day and under identical experimental conditions. In contrast, lymphocytes from many patients with malignant disease had decreased SLM activity, and this decrease was highly significant in patients with treated or untreated metastatic disease, or untreated CLL. This was also the case when the data were expressed relative to the number of cytotoxic cells in the normal control population, or in comparison to the relative SLMC activity of lymphocytes from patients with other conditions. Markedly decreased SLMC was observed in some patients in spite of normal T and B lymphocyte proportions, or the presence of the ability to mount a vigorous delayed hypersensitivity reaction to PPD. A comparison of the xenogeneic and allogeneic assays showed that the same information with respect to whether SLMC was normal or abnormal was obtained with both assays in the majority of cases. The significance of the data is discussed with respect to the possible role of SLMC in vivo and the relevance of SLMC to the assessment of specific cell-mediated cytotoxicity in malignant disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Spontaneous human lymphocyte‐mediated cytotoxicity againts tumour target cells. I. The effect of malignant disease

Pross

Baines

1976

Intl Journal of Cancer

238

View full text Add to dashboard Cite

show abstract

“…These results are similar to the findings of competitive inhibition of Fc-receptor-bearing K cells by (Fc + C3 +) tonsillar lymphocytes. 60 50 LO 30 20 derived lymphocyte (de Vries et al, 1974;Matthews and MacLaurin, 1975;Peter et al, 1975;Pross and Jondal, 1975;Kiuchi and Takasugi, 1976;Parkman and Rosen, 1976).…”

Section: Competitive Inhibition By (Fc + C3 +) Lymphocytes In Naturalmentioning

confidence: 99%

“…20 derived lymphocyte (de Vries et al, 1974;Matthews and MacLaurin, 1975;Peter et al, 1975;Pross and Jondal, 1975;Kiuchi and Takasugi, 1976;Parkman and Rosen, 1976).…”

mentioning

confidence: 99%

Characterization of the human natural killer (NK) cell in blood and lymphoid organs

Eremin

Coombs

Plumb

et al. 1978

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…A number of laboratories worked concurrently on the problem of effectot cellidentification (DeVries et al, 1974(DeVries et al, , 1975Herseyet al, 1975;Jondal andPross, 1975, Matthews andMacLaurin, 1975;Peter et al, 1975bPeter et al, , 1976aPross and Jondal, 1975a, b), and much of this work has been confirmed (Kiuchi and Takasugi, 1976;Peter et al, 1976b, c;Bak~cs et al, 1977a, b,c;Westet al, 1977;Prosset al, 1977a). A number of laboratories worked concurrently on the problem of effectot cellidentification (DeVries et al, 1974(DeVries et al, , 1975Herseyet al, 1975;Jondal andPross, 1975, Matthews andMacLaurin, 1975;Peter et al, 1975bPeter et al, , 1976aPross and Jondal, 1975a, b), and much of this work has been confirmed (Kiuchi and Takasugi, 1976;Peter et al, 1976b, c;Bak~cs et al, 1977a, b,c;Westet al, 1977;Prosset al, 1977a).…”

mentioning

confidence: 99%