Spontaneous calcium transients in the immature adult-born neurons of the olfactory bulb

Maslyukov, Anatoliy; Li, Kaizhen; Su, Xin; Kovalchuk, Yury; Garaschuk, Olga

doi:10.1016/j.ceca.2018.06.001

Cited by 7 publications

(18 citation statements)

References 74 publications

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“…spiking) when their Twitch-2B ratio is above 2.4 and nonactive (i.e. electrically silent) when their Twitch-2B ratio is below 2.0 41 (Fig. 5d) was similar between the subsequently eliminated and surviving ABCs at all 3 time points.…”

Section: Distinct Endogenous Activity Levels In Eliminated and Survivmentioning

confidence: 76%

“…5). Our previous study described the ubiquitous presence of spontaneous Ca 2+ transients in the Twitch-2B + adult-born JGNs 41 . Using tetrodotoxin, the blocker for voltage-gated Na + channels, we established that Twitch-2B + ABCs can be considered as active (i.e.…”

Section: Distinct Endogenous Activity Levels In Eliminated and Survivmentioning

confidence: 92%

“…indicator Twitch-2B 59 . Because of its ratiometric nature, Twitch-2B is particularly valuable for longitudinal imaging of individual cells in awake animals 41,59 . Since it is difficult to determine the identity of migrating adult-born JGNs without unique color tags, our longitudinal imaging started at DPI 18 ( Fig.…”

Section: Odor-responsiveness As a Potential Predictor Of Adult-born Jmentioning

confidence: 99%

“…The endogenous activity, represented by Ca 2+ spikes, is already present in migrating neuroblasts in the RMS and the subependymal layer of the bulb 39,40 . Spontaneous Ca 2+ signals are also ubiquitous in ABCs which have reached their destination layer 41 . The cell-intrinsic activity has been shown to influence the fate of adult-born GCs, with genetically enhanced cell-intrinsic activity (by expression of the voltage-gated Na + channel NaChBac) increasing and genetically suppressed cellintrinsic activity (by expression of Kir2.1 K + channel) decreasing their survival 35 .…”

Section: Introductionmentioning

confidence: 99%

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Enhanced endogenous activity predicts elimination of adult-born neurons in the mouse olfactory bulb

Kovalchuk

Mojtahedi

et al. 2020

Preprint

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Adult-born cells, arriving daily into the rodent olfactory bulb, either integrate into the neural circuitry or get eliminated. Whether these two populations differ in their morphological or functional properties remains, however, unclear. Using in vivo two-photon imaging, we monitored longitudinally the dendritic morphogenesis, odor-evoked responsiveness, endogenous Ca2+ signaling and survival/death of adult-born juxtaglomerular neurons (JGNs). We found that JGN maturation is accompanied by a significant reduction in dendritic complexity, with surviving and subsequently eliminated cells showing similar degrees of reduction and dendritic remodeling. Moreover, ∼63% of subsequently eliminated adult-born JGNs acquired odor-responsiveness before death, with amplitudes and time courses of odor-evoked responses similar to those recorded in the surviving cells. We observed, however, a significant long-lasting enhancement of the endogenous Ca2+ signaling in subsequently eliminated JGNs, visible already 6 days before death. These findings identify the ongoing endogenous Ca2+ signaling as a key predictor of the adult-born JGN’s fate.

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Section: Distinct Endogenous Activity Levels In Eliminated and Survivmentioning

confidence: 76%

Section: Distinct Endogenous Activity Levels In Eliminated and Survivmentioning

confidence: 92%

Section: Odor-responsiveness As a Potential Predictor Of Adult-born Jmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced endogenous activity predicts elimination of adult-born neurons in the mouse olfactory bulb

Kovalchuk

Mojtahedi

et al. 2020

Preprint

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“…In parallel, Ca 2+ imaging experiments unveiled the existence in NSCs, of intercellular Ca 2+ waves that are propagated through gap junctions and that are used by NSCs to communicate with each other and with neighboring niche astrocytes both under physiological conditions (Lacar et al, 2011) and pathological conditions (Kraft et al, 2017). Live cell Ca 2+ imaging has also allowed to observe spontaneous Ca 2+ oscillations in NSC/progenitors from the V-SVZ (Domenichini et al, 2018) as well as spontaneous high-frequency transients in migrating neuroblasts (Maslyukov et al, 2018). Along with the prominence of transcripts related to the Ca 2+ pathway in NSCs, these data lend support to the essential role of Ca 2+ in NSCs and their progeny.…”

Section: Physiological Roles Of Ca 2+ In Nscs and Their Progeniesmentioning

confidence: 98%

Calcium Channels in Adult Brain Neural Stem Cells and in Glioblastoma Stem Cells

Coronas

Terrié

Déliot

et al. 2020

Front. Cell. Neurosci.

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Endogenous but not sensory-driven activity controls migration, morphogenesis and survival of adult-born juxtaglomerular neurons in the mouse olfactory bulb

Figarella

et al. 2023

Cell. Mol. Life Sci.

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The development and survival of adult-born neurons are believed to be driven by sensory signaling. Here, in vivo analyses of motility, morphology and Ca2+ signaling, as well as transcriptome analyses of adult-born juxtaglomerular cells with reduced endogenous excitability (via cell-specific overexpression of either Kv1.2 or Kir2.1 K+ channels), revealed a pronounced impairment of migration, morphogenesis, survival, and functional integration of these cells into the mouse olfactory bulb, accompanied by a reduction in cytosolic Ca2+ fluctuations, phosphorylation of CREB and pCREB-mediated gene expression. Moreover, K+ channel overexpression strongly downregulated genes involved in neuronal migration, differentiation, and morphogenesis and upregulated apoptosis-related genes, thus locking adult-born cells in an immature and vulnerable state. Surprisingly, cells deprived of sensory-driven activity developed normally. Together, the data reveal signaling pathways connecting the endogenous intermittent neuronal activity/Ca2+ fluctuations as well as enhanced Kv1.2/Kir2.1 K+ channel function to migration, maturation, and survival of adult-born neurons.

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Spontaneous calcium transients in the immature adult-born neurons of the olfactory bulb

Cited by 7 publications

References 74 publications

Enhanced endogenous activity predicts elimination of adult-born neurons in the mouse olfactory bulb

Enhanced endogenous activity predicts elimination of adult-born neurons in the mouse olfactory bulb

Calcium Channels in Adult Brain Neural Stem Cells and in Glioblastoma Stem Cells

Endogenous but not sensory-driven activity controls migration, morphogenesis and survival of adult-born juxtaglomerular neurons in the mouse olfactory bulb

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