Spontaneous-assembly of phospholipid monolayers via adsorption onto gold

Diem, Tadeusz; Czajka, B.; Weber, Bruce A.; Regen, Steven L.

doi:10.1021/ja00279a099

Cited by 26 publications

(9 citation statements)

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“…Thanks to these holes the ten-point-height (S z ) roughness parameter gives a measure for the mean thickness of the binary lipid monolayer. The observed value of 4.0 nm seems to closely correspond to a superposition of the theoretical monolayer thickness (2.8 nm) [3,16] and the maximal height differences of the substrate (1.4 nm). In addition to the holes in the topographic image, the phase contrast image ( Figure 4B) reveals more structural grain boundaries in the monolayer.…”

Section: Follow-up Of the Label Amount By Tof-simssupporting

confidence: 67%

Analysis of Supramolecular Protein Assemblies on Binary Lipid Monolayers by Adsorption, Topographical, and Fluorescence Studies

Ihalainen

Laitala

Hemmilä

et al. 2007

Journal of Dispersion Science and Technology

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Binary films of 1-palmitoyl-2-(16-(S-methyldithio)-hexadecanoyl)-sn-glycero-3-phosphocholine (DSDPPC) and 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(Cap Biotinyl) (biotin-Cap-DPPE) were formed at the air-water interface. These floating Langmuir monolayers were transferred to a solid gold-coated substrate by the Langmuir-Schaefer deposition method. The role of the DSDPPC lipids is to bind the monolayer covalently to the gold substrate. The embedded receptor molecules (biotin-Cap-DPPE) make the film reactive against streptavidin (SA). For the deposition of subsequent streptavidin layers, a film of biotinylated bovine serum albumin (BBSA) was utilized as an intermediate binding layer between the consecutive streptavidin monolayers. The structure of the deposited binary lipid monolayer as well as the immobilization of subsequent protein layers was studied by Quartz Crystal Microbalance (QCM), Scanning Probe Microscopy (SPM) and ToF-SIMS. Attention was paid on the de-convolution and roughness analysis of the SPM data in order to obtain more quantitative information about the immobilized proteins. Fluorescence from the Eu-labeled SA was finally studied as a function SA-gold substrate distance.

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Section: Follow-up Of the Label Amount By Tof-simssupporting

confidence: 67%

Analysis of Supramolecular Protein Assemblies on Binary Lipid Monolayers by Adsorption, Topographical, and Fluorescence Studies

Ihalainen

Laitala

Hemmilä

et al. 2007

Journal of Dispersion Science and Technology

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“…Figure 7. Examples for polyzwitterions produced via step growth polymerization: (a) polyurethanes [376]; (b) polycarbonates [380]; (c) polysiloxanes [381] Other reported examples of step growth polymerizations toward polyzwitterions (Figure 8) seem rather exotic at the first view, such as radical polyadditions of isolated dienes with bisthiols via thiol-ene reaction [382], or the oxidative coupling of bisthiols [383][384][385][386][387]. Yet, for the reasons discussed in Section 3.1.1, such methods seem particularly attractive from the chemical point of view, as they are not affected by ionic compounds.…”

Section: Synthesis By Step Growth Polymerizationsmentioning

confidence: 99%

Structures and Synthesis of Zwitterionic Polymers

2014

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Abstract:The structures and synthesis of polyzwitterions ("polybetaines") are reviewed, emphasizing the literature of the past decade. Particular attention is given to the general challenges faced, and to successful strategies to obtain polymers with a true balance of permanent cationic and anionic groups, thus resulting in an overall zero charge. Also, the progress due to applying new methodologies from general polymer synthesis, such as controlled polymerization methods or the use of "click" chemical reactions is presented. Furthermore, the emerging topic of responsive ("smart") polyzwitterions is addressed. The considerations and critical discussions are illustrated by typical examples.

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“…Examples for zwitterionic and ionic long-chain alkyl thiols used for preparing zwitterionic and pseudozwitterionic SAMs on gold surfaces. ,− …”

Section: Preparing Zwitterionic Model Surfacesmentioning

confidence: 99%

Molecular Design of Zwitterionic Polymer Interfaces: Searching for the Difference

2018

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The widespread occurrence of zwitterionic compounds in nature has incited their frequent use in designing biomimetic materials. Therefore, zwitterionic polymers are a thriving field. A particular interest for this particular polymer class has currently focused on their use in establishing neutral, low-fouling surfaces. After highlighting strategies to prepare model zwitterionic surfaces as well as those that are more suitable for practical purposes relying strongly on radical polymerization methods, we present recent efforts to diversify the structure of the hitherto quite limited variety of zwitterionic monomers and of the derived polymers. We identify key structural variables, consider their influence on essential properties such as overall hydrophilicity and long-term stability, and discuss promising targets for the synthesis of new variants.

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Spontaneous-assembly of phospholipid monolayers via adsorption onto gold

Cited by 26 publications

References 0 publications

Analysis of Supramolecular Protein Assemblies on Binary Lipid Monolayers by Adsorption, Topographical, and Fluorescence Studies

Analysis of Supramolecular Protein Assemblies on Binary Lipid Monolayers by Adsorption, Topographical, and Fluorescence Studies

Structures and Synthesis of Zwitterionic Polymers

Molecular Design of Zwitterionic Polymer Interfaces: Searching for the Difference

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