RSC Adv.
 2023
DOI: 10.1039/d3ra04591a
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Spontaneous and site-specific immobilization of PNGase F via spy chemistry

Liang Zhang,
Wenhui Wang,
Yueqin Yang
et al.

Abstract: Recombinant PNGase F was spontaneously, site-specifically, and covalently immobilized onto magnetic nanoparticles based on Spy chemistry. The immobilized PNGasae F exhibited excellent deglycosylation activity, thermostability, and reusability.

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