Sponge holobionts shift their prokaryotic communities and antimicrobial activity from shallow to lower mesophotic depths

Indraningrat, Anak Agung Gede; Steinert, Georg; Becking, Leontine E.; Mueller, Benjamin; Goeij, Jasper M. de; Smidt, Hauke; Sipkema, Detmer

doi:10.1007/s10482-022-01770-4

Cited by 7 publications

(6 citation statements)

References 112 publications

(134 reference statements)

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“…These differences were mainly attributed to the relative abundance of the bacterial orders rather than their identity, which was similar at all depths (Figure 3A). These findings are in line with previous studies that compared microbial assemblages in relation to depth, and revealed similar patterns (Morrow et al, 2016;Indraningrat et al, 2022). Along the shallow to mesophotic depths the physical, biochemical, and biotic factors undergo change (Lesser et al, 2018).…”

Section: Mesophotic and Shallow Microbial Communities: General Descri...supporting

confidence: 92%

“…Many bacterial members have been found to change in abundance between the wild-type shallow and mesophotic sponges. For example, ASV members of the order Caldilineales (Chloroflexi) were enriched in the wild-shallow samples, as found for the sponges X. muta and Agelas sventres (Indraningrat et al, 2022). In the mesophotic sponges, a higher abundance of Nitrospirota bacteria was found (Figure 3A).…”

Section: Mesophotic and Shallow Microbial Communities: General Descri...mentioning

confidence: 71%

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A comparison of mesophotic and shallow sponge holobionts resilience to predicted future temperature elevation

et al. 2023

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Climate change is predicted to have detrimental impacts on sessile invertebrates, including sponges. Mesophotic ecosystems have been suggested to play a major role as refugia for coral reef sponge species, however knowledge regarding the ability of mesophotic sponges to cope with thermal stress is scarce. In this study we compared the response of the sponge Diacarnus erythraeanus, a widespread Red Sea sponge, from the shallow and mesophotic reef, to moderate and acute temperature elevation (2°C and 6°C, respectively) for short and long term periods (two and 35 days, respectively) by measuring physiological parameters (respiration, oxygen removal, pumping rates, and photosynthetic efficiency), and the microbiome composition change. The results indicated that mesophotic and shallow populations of D. erythraeanus are highly tolerant to both moderate and acute heat stress, demonstrating a high survival rate (100%) across the experimental treatments, with no visible signs of bleaching or necrosis. Exposure to heat stress resulted in significant alterations in the physiological parameters of sponges, including higher respiration rate and lower photosynthetic efficiency. These alterations were accompanied by correspondingly significant microbial adjustments, thus emphasizing the essential role of the microbiome in the host’s ability to persist when facing essential environmental stress. Moreover, while shallow and mesophotic sponges showed similar physiological tolerance to heat stress, their microbial response differed: while the microbiome diversity of the mesophotic sponges remained stable throughout the experiment, the shallow one significantly changed. This result suggests that their underlying coping mechanisms might differ between mesophotic and shallow populations. Since the associated-microbiome is largely regulated by the sponge-host genetics, difference in microbial adjustments to stress between populations, could indicate genetic variability between hosts. Therefore, while the results of this study support the hypothesis that mesophotic coral reefs could serve as thermal refugia for some sponge species, it raises the question regarding the validity of MCEs as a refuge for shallow populations. Finally, it emphasizes the crucial need to elucidate the underlying mechanisms governing the sponge-microbiome interactions, specifically in the context of the anticipated climate change scenarios.

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Section: Mesophotic and Shallow Microbial Communities: General Descri...supporting

confidence: 92%

Section: Mesophotic and Shallow Microbial Communities: General Descri...mentioning

confidence: 71%

A comparison of mesophotic and shallow sponge holobionts resilience to predicted future temperature elevation

et al. 2023

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“…In addition, the SVA0996 marine group has also been detected in the mucus, tissue, and skeleton of the reef coral Porites lutea (Kuang et al 2015), the Mediterranean coral, Astroides Fig. 8 Non-metric multidimensional scaling (nMDS) plots for the A Agulhas National Park (ANP) samples and the B Springfield Farm (Areas 1-3) samples, showing the actinobacterial community similarities and the key environmental drivers for these communities calycularis (Biagi et al 2020), found in association with marine sponges (Slaby et al 2017;Turon et al 2019;Verhoeven 2019;Indraningrat et al 2022), found in association with the brown alga, Laminaria digitata (Ihua et al 2020), the macro-alga, Taonia atomaria (Paix et al 2019), and ascidians (Tunicata) (Steinert et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Although the role of the Sva0996 marine group in sponges is unknown, Orsi et al (2016) showed that this group is involved in dissolved protein cycling in the ocean, while Indraningrat et al (2022) reported that this taxon may be present where high nitrate concentrations are found, as well as in high productivity areas. A study by Biagi et al (2020) confirmed the potential of this marine group to utilise organic nitrogen, where it has been shown that stressed reef-building corals produce mucus with a higher protein content.…”

Section: Discussionmentioning

confidence: 99%

Actinobacteria diversity associated with marine sediments and a wetland system, Agulhas-South Africa

Prins,

Dyani,

Vreulink

et al. 2024

Ann Microbiol

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Background South Africa is known for its great biodiversity. The Agulhas Plain represents one such unique environment where low-gradient topography has resulted in extensive wetland formation. It is fed by two major river systems, bringing in brackish, alkaline water. It has been exposed to major marine transgression and regression events, and harbours great Fynbos diversity as well as a Mediterranean-type climate, thereby creating unique ecosystems. It is therefore surprising that little is known about the bacterial diversity associated with the Agulhas Plain and associated marine ecosystems. Methods In this study, we focused on the actinobacterial diversity (Phylum Actinomycetota) associated with an emerging peatland on the Agulhas Plain (SF; Areas 1–3) and a marine site (ANP; Ocean, Rocky, Dry) located 10 km away from SF. A combined metataxanomics and isolation approach was taken to evaluate the actinobacterial diversity of the sampling sites and to determine the effect of environmental physicochemical parameters on these populations. Various genome analyses were performed on an Sva0096 marine bin to gain insight into its ecological role. Results Metataxanomics showed that the two sites shared defined major taxa, including Blastococcus, Geodermatophilus, Microbacterium, Mycobacterium, Nocardioides, Streptomyces, and the Sva0996 marine group. Analysis of the biosynthetic potential of an Sva0996 marine bin134 (obtained from GenBank) provided insights into the potential ecological role of this group of bacteria in both the marine and terrestrial environments. Higher actinobacterial diversity (Shannon index > 5) was observed for Areas 2 and 3 (SF), as well as the ANP Dry samples. The actinobacterial population composition was found to be driven by salinity, pH, Mn, and Ca, with certain areas of SF exhibiting similar (and even higher) salinity (SF: 70–100 Ω vs. ANP: 100–160 Ω) and lower pH levels (SF: 6.3-8.0 vs. ANP: 8.6–8.9) to that of the marine environment. Conclusion This snapshot study has provided some insights into the actinobacterial diversity of the two sites studied. Analysis of an Sva0096 marine bin134 provided further insights into the potential ability of the Sva0096 marine group to survive in a unique terrestrial environment that is periodically exposed to environmental pressures that mimic the marine environment.

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“…The extraction of sponge samples was conducted following a previously published protocol, with adjustments made to the quantity of starting materials (Tsukamoto et al, 2010;Rohde et al, 2015;Indraningrat et al, 2022).…”

Section: Sponge Extractionmentioning

confidence: 99%

Identification of The Secondary Metabolite Derived from Aaptos spp. and its Antibacterial Activity

Utami,

Paramita,

Agustina

et al. 2024

Indonesian J Pharm

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Many novel bioactive compounds have been found to be most abundantly produced by sponges. Over the years, Indonesian sponges have played an important role in expanding our understanding of chemical diversity and biological activity. Among those sponges, the sponge genus Aaptos has been shown to have antimicrobial activity. Antimicrobial resistance and the spread of infections in the immunocompromised population have all contributed to an increase in the appearance and severity of these infections in recent decades. Finding an alternative antimicrobial drug from marine secondary metabolites will be beneficial. The current study looks into the profile of bioactive compounds derived from the sponge Aaptos. The sponge was collected from Raja Ampat Island, Indonesia. This sample was extracted with ethanol and an ethyl acetate-methanol solvent. Furthermore, Thin Layer Chromatography (TLC), High-performance liquid chromatography with diode-array detection (HPLC-DAD) and Principal Component Analysis (PCA) were used to map the secondary metabolite related to antimicrobial activity in both extracts. TLC profiling revealed potential alkaloid and terpenoid compounds from both ethanol extract and ethyl acetate-methanol. The HPLC-DAD and PCA results revealed that one of the ethanol crude extracts had a similar profile to Ciprofloxacin, Chloramphenicol, and Ampicillin. These antibacterial profiles open up new research avenues, with the ultimate goal of contributing to the development of new and effective antimicrobial drugs. This research also helps with the bioprospecting of marine sponges from Indonesian sea water.

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Sponge holobionts shift their prokaryotic communities and antimicrobial activity from shallow to lower mesophotic depths

Cited by 7 publications

References 112 publications

A comparison of mesophotic and shallow sponge holobionts resilience to predicted future temperature elevation

A comparison of mesophotic and shallow sponge holobionts resilience to predicted future temperature elevation

Actinobacteria diversity associated with marine sediments and a wetland system, Agulhas-South Africa

Identification of The Secondary Metabolite Derived from Aaptos spp. and its Antibacterial Activity

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