Sponge Bcl-2 homologous protein (BHP2-GC) confers distinct stress resistance to human HEK-293 cells

Wiens, Matthias; Diehl‐Seifert, Bärbel; Müller, Wernér E.G.

doi:10.1038/sj.cdd.4400906

Cited by 52 publications

(37 citation statements)

References 66 publications

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“…70 GCBHP2 expression is upregulated in sponge cells exposed to low Apical and initiator caspase homologs, pro-and antiapoptotic Bcl-2 family members. Caspase-dependent cell death observed in allograft rejection [67][68][69][70][71][72] (but not high) levels of tributyltin or heat shock, and GCBHP2 appears to have an antiapoptotic role. Mammalian cells transfected with GCBHP2 were somewhat resistant to serum starvation and tributylin-induced apoptosis.…”

Section: Out Of the Mainstream Into The Oceanmentioning

confidence: 99%

“…Mammalian cells transfected with GCBHP2 were somewhat resistant to serum starvation and tributylin-induced apoptosis. 71 In addition, a homolog of BAK (LBBAK2l) with homology in the BH1, BH3, and transmembrane domains and a homolog of Bcl-2 (LBBCL-2a) with homology in all four BH domains have been cloned. Expression of caspases and Bcl-2 family proteins has been analyzed in the developing sponge and it appears that the antiapoptotic proteins are expressed in the proliferative zone, whereas the proapoptotic proteins are expressed in the non-proliferative zone.…”

Section: Out Of the Mainstream Into The Oceanmentioning

confidence: 99%

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Living with death: the evolution of the mitochondrial pathway of apoptosis in animals

2008

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The mitochondrial pathway of cell death, in which apoptosis proceeds following mitochondrial outer membrane permeabilization, release of cytochrome c, and APAF-1 apoptosome-mediated caspase activation, represents the major pathway of physiological apoptosis in vertebrates. However, the well-characterized apoptotic pathways of the invertebrates C. elegans and D. melanogaster indicate that this apoptotic pathway is not universally conserved among animals. This review will compare the role of the mitochondria in the apoptotic programs of mammals, nematodes, and flies, and will survey our knowledge of the apoptotic pathways of other, less familiar model organisms in an effort to explore the evolutionary origins of the mitochondrial pathway of apoptosis.

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Section: Out Of the Mainstream Into The Oceanmentioning

confidence: 99%

Section: Out Of the Mainstream Into The Oceanmentioning

confidence: 99%

Living with death: the evolution of the mitochondrial pathway of apoptosis in animals

2008

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“…31 Sponges possess homologs of genes involved in apoptotic pathways in human cells. 32,33 However, molecular mechanism of apoptosis in sponges is yet unknown and Btk may play important role in deciding the fate of the cell depending on the biological response specific for the cell type.…”

Section: Protein Btksd From Marine Sponge S Domunculamentioning

confidence: 99%

Characterization of Bruton’s Tyrosine Kinase Gene and Protein from Marine Sponge Suberites domuncula

Perina¹,

Mikoč²,

Harcet³

et al. 2012

Croat. Chem. Acta

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Abstract. Bruton's tyrosine kinase (Btk) is a member of Tec family of protein tyrosine kinases. It is expressed in B cells and myeloid cells in humans and it is crucial for maturation of B-lineage lymphoid cells. Mutations in the gene encoding human Btk result in hereditary immunodeficiency X-linked agammaglobulinemia. Sponge proteins can probably reflect characteristics of the ancestral metazoan homologues before their diversifications and specializations in complex animals.Gene coding for sponge Btk-like protein was fully characterized and its structure was compared with homologous genes from other organisms. Kinase activity of sponge protein was compared to human Btk activity.We showed that sponge Btk-like gene is probably the most ancient member of Tec family while other members arose from the ancestral gene present in chordate ancestor. The ancestral-type protein was structurally and functionally similar to the highly sophisticated enzyme it is today, whose functions are usually associated with higher Metazoa. (doi: 10.5562/cca1862)

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“…This strategy had already been successfully adopted to assess the antiapoptotic potential of deterin and poriferan B-cell lymphoma (Bcl)-2. 31,32 Moreover, survivin-EGFP fusions have been widely used to study both the bifunctional role of survivin and its dynamic intracellular localization and seem fully functional. [33][34][35] In contrast to sponge tissue that show a very low steadystate expression level, proliferating cells of primmorphs contained a considerable amount of SDSURVL, indicative of its involvement in cell division.…”

Section: Discussionmentioning

confidence: 99%

Poriferan survivin exhibits a conserved regulatory role in the interconnected pathways of cell cycle and apoptosis

Luthringer¹,

Isbert²,

Müller³

et al. 2010

Cell Death Differ

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Survivin orchestrates intracellular pathways during cell division and apoptosis. Its central function as mitotic regulator and inhibitor of cell death has major implications for tumor cell proliferation. Analyses in early-branching Metazoa so far propose an exclusive role of survivin as a chromosomal passenger protein, whereas only later during evolution a complementary antiapoptotic function might have arisen, concurrent with increased organismal complexity. To lift the veil on the ancestral function(s) of this key regulator, a survivin-like protein (SURVL) of one of the earliest-branching metazoan taxa was identified and functionally characterized. SURVL of the sponge Suberites domuncula shares considerable similarities with its metazoan homologs, ranging from conserved exon/intron structure to presence of protein-interaction domains. Whereas sponge tissue shows a low steady-state level, SURVL expression was significantly upregulated in rapidly proliferating primmorph cells. In addition, challenge of tissue and primmorphs with heavy metal or lipopeptide stimulated SURVL expression, concurrent with the expression of a newly discovered caspase. Complementary functional analyses in transfected HEK-293 cells revealed that heterologous expression of a SURVL-EFGP fusion not only promotes proliferation but also enhances resistance to cadmiuminduced cell death. Taken together, these results suggest both a deep evolutionary conserved dual role of survivin and an equally conserved central position in the interconnected pathways of cell cycle and apoptosis. Apoptosis has a crucial role not only in countless physiological processes (e.g., elimination of redundant cells during vertebrate embryonic development) but also during pathogenesis. As apoptosis relies on the clear-cut function of numerous proand antiapoptotic factors, any malfunction on their part or disruption of their respective interplay inevitably leads to dysfunctional cellular metabolism. The ensuing delayed or premature cell death often manifests in severe pathologies, including carcinogenesis and neurodegenerative diseases.The groundbreaking discovery of the Caenorhabditis elegans proteins cell death abnormality (Ced)-9, homolog of human antiapoptotic Bcl-2, and Ced-3, homolog of human caspase-1, has shown the molecular conservation of the apoptotic cell death program along a broad range of Metazoa, from nematodes to vertebrates.1 Since then, the fundamental role of apoptosis has been increasingly recognized in various physiological processes. In addition, several invertebrate model organisms revealed a significant potential for analyses of apoptotic mechanisms, not only under an evolutionary point of view. Thus, the identification of the human crumbs homolog 1 and its causative role in the manifestation of retinitis pigmentosa was based on the discovery of mutational variants of the Drosophila melanogaster protein crumbs.2 In addition, loss-of-function mutations of the C. elegans dystrophin-1 gene confirmed the role of human dystrophin in the manifestation...

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Sponge Bcl-2 homologous protein (BHP2-GC) confers distinct stress resistance to human HEK-293 cells

Cited by 52 publications

References 66 publications

Living with death: the evolution of the mitochondrial pathway of apoptosis in animals

Living with death: the evolution of the mitochondrial pathway of apoptosis in animals

Characterization of Bruton’s Tyrosine Kinase Gene and Protein from Marine Sponge Suberites domuncula

Poriferan survivin exhibits a conserved regulatory role in the interconnected pathways of cell cycle and apoptosis

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