Spoltud-1 is a chromatoid body component required for planarian long-term stem cell self-renewal

Solana, Jordi; Lasko, Paul; Romero, Rafael

doi:10.1016/j.ydbio.2009.01.043

Cited by 83 publications

(101 citation statements)

References 70 publications

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“…Planarian homologues of piwi and pumilio genes were found specifically expressed in a neoblasts (Rossi et al, 2007) and the expression pattern of Piwi protein in planaria Schmidtea mediterranea coincides with the neoblasts (Reddien et al, 2005). The Tudor protein is a component of chromatoid bodies in germ cells and neoblasts in the planaria Schmidtea polychroa (Solana et al, 2009). Co-localization and co-expression of Piwi-and Tudor-related proteins also was detected in planarian neoblasts (see Shibata et al, 2010).…”

Section: Molecular Signature In Pluripotent Gametogenic Stem Cellsmentioning

confidence: 93%

“…Planarian (Plathyhelminthes) neoblasts can differentiate into germ and somatic cells and express vasa, piwi, nanos, pumilio, bruno, tudor homologues (Agata et al, 2006;Pfister et al, 2008;Reddien et al, 2005;Rossi et al, 2007;Shibata et al, 1999Shibata et al, , 2010Solana et al, 2009). In Dugesia japonica two vasa homologues are expressed in the germ cells of the adult gonads.…”

Section: Molecular Signature In Pluripotent Gametogenic Stem Cellsmentioning

confidence: 99%

“…During embryonic development of the planaria Schmidtea polychroa, Tudor-related protein is expressed in differentiating cells rather than neoblasts (Solana et al, 2009). …”

Section: Transient Expression Of Germline Marker Genes During Developmentioning

confidence: 99%

“…Some proteins classically related to germ line development have been recently found to be involved in neuronal function and development. In the planaria Schmidtea polychroa, Tudorrelated protein is expressed, beyond germline cells and neoblasts, in the central nervous system (Solana et al, 2009). pumilio and bruno planarian homologues are expressed similarly in neoblasts and in the central neural system, in perinuclear particles surrounding the nuclei of neurons (Salvetti et al, 2005;Guo et al, 2006).…”

Section: Germline Marker Gene Expression In Somatic Stem Pools and Nementioning

confidence: 99%

“…pumilio and bruno planarian homologues are expressed similarly in neoblasts and in the central neural system, in perinuclear particles surrounding the nuclei of neurons (Salvetti et al, 2005;Guo et al, 2006). nanos and pumilio are involved in neuronal excitability, dendrite morphogenesis, and long-term memory in D. melanogaster (see Muraro et al, 2008;Solana et al, 2009). When mouse ESCs were cultured in serum-and feederdeprived conditions colony-forming primitive neural stem cell populatiuons could be obtained (Stice et al, 2006).…”

Section: Germline Marker Gene Expression In Somatic Stem Pools and Nementioning

confidence: 99%

See 4 more Smart Citations

Molecular and Sub-Cellular Gametogenic Machinery of Stem and Germline Cells Across Metazoa

Shukalyuk¹,

Isaeva²

2012

Current Frontiers and Perspectives in Cell Biology

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Section: Molecular Signature In Pluripotent Gametogenic Stem Cellsmentioning

confidence: 93%

Section: Molecular Signature In Pluripotent Gametogenic Stem Cellsmentioning

confidence: 99%

“…During embryonic development of the planaria Schmidtea polychroa, Tudor-related protein is expressed in differentiating cells rather than neoblasts (Solana et al, 2009). …”

Section: Transient Expression Of Germline Marker Genes During Developmentioning

confidence: 99%

Section: Germline Marker Gene Expression In Somatic Stem Pools and Nementioning

confidence: 99%

Section: Germline Marker Gene Expression In Somatic Stem Pools and Nementioning

confidence: 99%

See 3 more Smart Citations

Molecular and Sub-Cellular Gametogenic Machinery of Stem and Germline Cells Across Metazoa

Shukalyuk¹,

Isaeva²

2012

Current Frontiers and Perspectives in Cell Biology

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An Introduction to Planarians and Their Stem Cells

Aboukhatwa

Aboobaker

2015

Encyclopedia of Life Sciences

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Of all animals, it is perhaps planarians that have the greatest ability to regenerate. These once classical classroom organisms have now become the subject of advanced molecular genetic studies that have the potential to inform us about our own biology. Regeneration relies upon a population of pluripotent adult stem cells, called neoblasts. These collectively pluripotent stem cells underpin the regenerative capacity of planarians. The underlying molecular mechanisms controlling their proliferation, self‐renewal and differentiation are now being described. In particular, progress has been made in describing the expression profile of these cells and identifying some of the molecular mechanisms that regulate their behaviour leading to a better understanding of how these cells power regeneration. Key Concepts Planarians are bilaterians and share many cell and tissue types with other animals including vertebrates. Planarians are able to mount a profound regenerative response to wounding allowing even small fragments to regenerate whole new worms. Planarian regeneration requires the proliferation and differentiation of a population of pluripotent stem cell population called neoblasts. Expression profiling of the neoblast population shows they are a heterogeneous population and express gene regulatory networks in common with both somatic and germline stem cells Many cell biological and molecular genetic approaches can be used to study gene function in planarians allowing novel biological insights to be made, relevant to our own biology. Current research has uncovered many important regulatory processes that regulate stem cells, including those regulated by transcription factors, chromatin modification and RNA binding proteins. Future work will uncover the detailed mechanisms that control stem cell proliferation, self‐renewal and differentiation as well as how missing structures are accurately restored.

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Ultrastructural and cytochemical aspects of the germarium and the vitellarium in Syndesmis patagonica (Platyhelminthes, Rhabdocoela, Umagillidae)

Falleni

Lucchesi

Ghezzani

et al. 2014

Journal of Morphology

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The cytoarchitecture of the female gonad of the endosymbiont umagillid Syndesmis patagonica has been investigated using electron microscopy and cytochemical techniques. The female gonad consists of paired germaria and vitellaria located behind the pharynx in the mid-posterior region of the body. Both the germaria and the vitellaria are enveloped by an outer extracellular lamina and an inner sheath of accessory cells which contribute to the extracellular lamina. Oocyte maturation occurs completely during the prophase of the first meiotic division. Oocyte differentiation is characterized by the appearance of chromatoid bodies and the development of endoplasmic reticulum and Golgi complexes. These organelles appear to be involved in the production of round granules, about 2-2.5 μm in diameter, with a homogeneous electron-dense core surrounded by a granular component and a translucent halo delimited by a membrane. These egg granules migrate to the periphery of mature oocytes, are positive to the cytochemical test for polyphenol detection, are unaffected by protease and have been interpreted as eggshell granules. The mature oocytes also contain a small number of yolk granules, lipid droplets, and glycogen particles scattered throughout the ooplasm. The vitellaria are branched organs composed of vitelline follicles with vitellocytes at different stages of maturation. Developing vitellocytes contain well-developed rough endoplasmic reticulum and small Golgi complexes involved in the production of eggshell and yolk globules. Eggshell globules are round, measure 4-5 μm in diameter, and have a mosaic-like patterned content which contains polyphenols. The yolk globules, 2-3 μm in diameter, show a homogeneous protein content of medium electron density, devoid of polyphenols, and completely digested by protease. The mature vitellocytes also contain glycogen as further reserve material. The presence of polyphenolic eggshell granules in the oocytes and of polyphenolic eggshell globules with a mosaic-like pattern in the vitellocytes have been considered apomorphic features of the Rhabdocoela + Prolecithophora.

show abstract

Spoltud-1 is a chromatoid body component required for planarian long-term stem cell self-renewal

Cited by 83 publications

References 70 publications

Molecular and Sub-Cellular Gametogenic Machinery of Stem and Germline Cells Across Metazoa

Molecular and Sub-Cellular Gametogenic Machinery of Stem and Germline Cells Across Metazoa

An Introduction to Planarians and Their Stem Cells

Ultrastructural and cytochemical aspects of the germarium and the vitellarium in Syndesmis patagonica (Platyhelminthes, Rhabdocoela, Umagillidae)

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