Spoilage potentials and antimicrobial resistance of Pseudomonas spp. isolated from cheeses

Abstract

Pseudomonas spp. are aerobic, gram-negative bacteria that are recognized as major food spoilage microorganisms. A total of 32 (22.9%) Pseudomonas spp. from 140 homemade white cheese samples collected from the open-air public bazaar were isolated and characterized. The aim of the present study was to investigate the biochemical characteristics, the production of extracellular enzymes, slime and β-lactamase, and antimicrobial susceptibility of Pseudomonas spp. isolated from cheeses. The identified isolates including Pseudomonas pseudoalcaligenes, Pseudomonas alcaligenes, Pseudomonas aeruginosa, Pseudomonas fluorescens biovar V, and P. pseudoalcaligenes ssp. citrulli were found to produce extracellular enzymes, respectively: protease and lecithinase production (100%), and lipase activity (85.7, 42.9, 100, and 100%, and nonlipolytic, respectively). The isolates did not produce slime and had no detectable β-lactamase activity. The antimicrobial susceptibility of the isolates was tested using the disk diffusion method. Pseudomonas spp. had the highest resistance to penicillin G (100%), then sulphamethoxazole/trimethoprim (28.1%). However, all Pseudomonas spp. isolates were 100% susceptible to ceftazidime, ciprofloxacin, amikacin, gentamicin, and imipenem. Multidrug-resistance patterns were not observed among these isolates. In this study, Pseudomonas spp., exhibiting spoilage features, were isolated mainly from cheeses. Isolation of this organism from processed milk highlights the need to improve the hygienic practices. All of the stages in the milk processing chain during manufacturing have to be under control to achieve the quality and safety of dairy products.

Inhibition of food-related bacteria by antibacterial substances produced by Pseudomonas sp. strains isolated from pasteurized milk

“…isolated from bulk tank milk samples were the dominant non-coliform Gram-negative bacteria and showed the highest levels of resistance to antibiotics. In a recent study, Arslan et al (2011) verified that all 32 Pseudomonas strains isolated from cheese were susceptible to ciprofloxacin, gentamicin and imipenem. Antibiotic-resistant Pseudomonas spp.…”

“…Some differences in the diameters of the inhibition zones were observed in this experiment, but all the staphylococcal strains were inhibited by these Pseudomonas strains. Strain 8.2 was not included in these tests, since its spectrum of action and the plasmidial DNA profile (data not shown) suggested that this strain was identical to strain 8.1. literature, resistance to antibiotics has become common amongst strains isolated from milk and dairy products (ARSLAN et al, 2011;BEENA et al, 2011). Straley et al (2006) verified that Pseudomonas spp.…”

Evaluation of the Extracts of Piper guineense for Antibacterial Activity Against Spoilage Bacteria of Rivers State ‘Native’ Soup.

“…The result of the potentials of individual and a mixed bacteria culture to cause spoilage of 'native' soup is presented in Table 2. The average spoilage time for all seeded soups ranged from 15.15 to 16.00 h compared to the control samples' average spoilage time of 18.15 h. There is a dearth of information on the spoilage microorganisms of Nigeria soups, however, a number of Authors have reported P. aeruginosa, Alcaligenes faecalis and Proteus vulgaris as major food spoilage bacteria of 'ugba' (African oil bean seeds), meat and dairy [27,28,29,30,31,32,33].…”

Decontamination Strategies for a Few Potentially Pathogenic and Food Spoilage Causing, Antibiotic-Resistant Bacteria Isolated From Commonly Used Food Garnishes

“…Genotypic analysis was not included because the objective of the study was to aim at eliminating any potentially pathogenic or food spoilage causing bacteria that may be present on food garnishes rather than target and eliminate specific bacteria. All the isolated organisms are known to cause food poisoning and/or food spoilage (Adams, 2000;Arslan, 2011, Doran, 1999Drake, 2007;Frost, 1995;Lund, 1993;Sabota, 1998;Silbernagel, 2001;Wogu, 2014).…”