Split-sciatic nerve surgery: A new microsurgical model in experimental nerve repair

Schiraldi, Luigi; Sottaz, Lima; Madduri, Srinivas; Campisi, C.; Oranges, Carlo M.; Raffoul, Wassim; Kalbermatten, Daniel F.; Summa, Pietro G. di

doi:10.1016/j.bjps.2017.11.007

Cited by 4 publications

(7 citation statements)

References 26 publications

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“…Two types of conduits were used in the experiments; those made of Reperen (Icon Lab, Russia) were supplied as ready-made sterile tubes; the fibrin glue conduits were prepared in this lab using a Tissucol Kit (Baxter, Austria) according to the well-known method [ 23 , 27 , 28 ].…”

Section: Methodsmentioning

confidence: 99%

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Reconstruction of the Rat Sciatic Nerve by Using Biodegradable and Non-Biodegradable Conduits

Velichanskaya¹,

Абросимов²,

Bugrova³

et al. 2020

Sovrem Tehnol Med

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The aim of the study was to compare two types of conduits made of either non-resorbable Reperen or resorbable Tissucol for their effects on the regeneration of the rat sciatic nerve under conditions of stump diastasis. Materials and Methods. The experiments were carried out using outbred white male rats of the reproductive age (n=14). The animals were divided into three groups: group 1, intact (n=5), used for studying the morphology of the sciatic nerve; group 2 (n=4) — nerve plastic surgery was performed using a conduit made of non-resorbable Reperen; group 3 (n=5) — surgery was performed using a conduit made of resorbable Tissucol. The animals were anesthetized with isoflurane. After a complete transection of the sciatic nerve in the middle third of the thigh, its stumps were inserted into a conduit of an internal diameter of 2 mm and a length of 10 mm, filled with saline. Diastasis of 5 mm in length was created by spreading the nerve ends and securing the epineurium to the tube edges with 8/0 polypropylene sutures. A total count of myelinated nerve fibers was performed in the area of repair (tubulation) and the distal part of the nerve; the formation of connective tissue sheaths was assessed 14 weeks after the operation. Results. According to the morphological assessment, both types of conduits (resorbable and non-resorbable) caused the similar number of fibers to restore in the distal part of the repaired nerve; clinical characteristics of the animals in both groups were close to each other and to the norm. Conclusion. The results allow us to consider the conduit made of non-resorbable Reperen as a device promising for neuroplasty along with the resorbable conduit made of Tissucol.

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Section: Methodsmentioning

confidence: 99%

“…There are reports on using unfilled biodegradable and non-biodegradable tubes to study the effect of various fillers on nerve regeneration [ 5 , 23 , 24 , 27 – 32 ]. Good results were obtained with biodegradable conduits, including those made of Tissucol fibrin glue [ 27 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of the Rat Sciatic Nerve by Using Biodegradable and Non-Biodegradable Conduits

Velichanskaya¹,

Абросимов²,

Bugrova³

et al. 2020

Sovrem Tehnol Med

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“…These cells showed greater expression of specific markers for angiogenesis, axonal fiber and myelin sheath, in addition to better muscle activity, compared to the group treated only with collagen membrane and fibrin glue. Corroborating these data, the regenerative potential of MSCs differentiated into neuronal cells has been reported in several studies with animal models [ 13 , 14 , 15 , 16 ]. Therefore, there is evidence in the literature that therapy with MSCs has the potential to favor nerve regeneration, regardless of the stage of cell differentiation [ 60 ].…”

Section: Discussionmentioning

confidence: 73%

“…Thus, nerve regeneration process is marked by overexpression of genes related to the inflammatory response, angiogenesis, synthesis of neurotrophic factors and expression of proteins involved in axonal growth and nerve fiber myelination [ 4 ]. Considering these issues, Schwann cells constitutes one of the most promising strategies to favor the regeneration of nervous tissue [ 5 , 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Potential of Fibrin Glue and Mesenchymal Stem Cells (MSCs) to Regenerate Nerve Injuries: A Systematic Review

Ortiz

Pomini

Bellini

et al. 2022

Cells

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Cell-based therapy is a promising treatment to favor tissue healing through less invasive strategies. Mesenchymal stem cells (MSCs) highlighted as potential candidates due to their angiogenic, anti-apoptotic and immunomodulatory properties, in addition to their ability to differentiate into several specialized cell lines. Cells can be carried through a biological delivery system, such as fibrin glue, which acts as a temporary matrix that favors cell-matrix interactions and allows local and paracrine functions of MSCs. Thus, the aim of this systematic review was to evaluate the potential of fibrin glue combined with MSCs in nerve regeneration. The bibliographic search was performed in the PubMed/MEDLINE, Web of Science and Embase databases, using the descriptors (“fibrin sealant” OR “fibrin glue”) AND “stem cells” AND “nerve regeneration”, considering articles published until 2021. To compose this review, 13 in vivo studies were selected, according to the eligibility criteria. MSCs favored axonal regeneration, remyelination of nerve fibers, as well as promoted an increase in the number of myelinated fibers, myelin sheath thickness, number of axons and expression of growth factors, with significant improvement in motor function recovery. This systematic review showed clear evidence that fibrin glue combined with MSCs has the potential to regenerate nervous system lesions.

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“…Right after sacrificing the rats, gastrocnemius muscles were carefully dissected and harvested from both intact (non-operative) and injured (operative) sides and weighed while they were still wet. In order to determine the weight ratios (in percentages), the weights of muscle mass from the injured sides were divided by those from the normal sides (Schiraldi et al, 2018[39]).…”

Section: Methodsmentioning

confidence: 99%

Comparison of melatonin and curcumin effect at the light and dark periods on regeneration of sciatic nerve crush injury in rats

Kasmaie

Jahromi

Gazor

et al. 2019

EXCLI Journal; 18:Doc653; ISSN 1611-2156

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Split-sciatic nerve surgery: A new microsurgical model in experimental nerve repair

Cited by 4 publications

References 26 publications

Reconstruction of the Rat Sciatic Nerve by Using Biodegradable and Non-Biodegradable Conduits

Reconstruction of the Rat Sciatic Nerve by Using Biodegradable and Non-Biodegradable Conduits

Potential of Fibrin Glue and Mesenchymal Stem Cells (MSCs) to Regenerate Nerve Injuries: A Systematic Review

Comparison of melatonin and curcumin effect at the light and dark periods on regeneration of sciatic nerve crush injury in rats

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