Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system

Abstract

A split G-quadruplex-programmed label-free Cas12a sensing platform was fabricated.

Split G-quadruplex based PfAgo sensing platform for nucleotide mutation discrimination and human genotyping

“…Apart from the common intra-stranded G4, inter-stranded G4, designated as split G4, could also refold into a G4 structure with the help of external links. 12 In split G4, external conformational reconfiguration stimuli are independent from the conserved G4 sequence, and hence exhibit superior flexibility in structural design and application potential in constructing a functional system. 13…”

“…To achieve the best split G4 performance, four nucleotides were employed here as spacers. 12 Firstly, the assembly of split G4 was characterized with native PAGE. As shown in Fig.…”

Simultaneous and multiplexed phenotyping of circulating exosomes with the orthogonal CRISPR–Cas platform

“…The clustered regularly interspaced short palindromic repeat (CRISPR) technology has paved an innovative way for nucleic acid detection. 13 Owing to their intrinsic specificity and sensitivity, platforms based on Cas9, Cas12a, [14][15][16] Cas13, 17,18 and Cas14 19 have all been established and implemented for biomedical analysis. Recently, CRISPR/Cas-based multiplex biosensing has been attracting more and more research interests.…”