Splicing of a circular yeast pre-mRNA<i>in vitro</i>

Schindewolf, Catherine; Domdey, Horst

doi:10.1093/nar/23.7.1133

Cited by 10 publications

(7 citation statements)

References 40 publications

(33 reference statements)

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“…6). In line with this, it is worth noting that synthetic circular pre-mRNAs have been shown to be spliced correctly in vitro (19). Furthermore, the demonstration that higher eukaryotic cells have the potential for trans-splicing both in vivo and in vitro (11)(12)(13) is consistent with the possibility of branched intermediates being capable to undergo additional splicing events.…”

Section: Discussionsupporting

confidence: 56%

Circular RNAs from transcripts of the rat cytochrome P450 2C24 gene: correlation with exon skipping.

Zaphiropoulos

1996

Proc. Natl. Acad. Sci. U.S.A.

218

190

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Section: Discussionsupporting

confidence: 56%

Circular RNAs from transcripts of the rat cytochrome P450 2C24 gene: correlation with exon skipping.

Zaphiropoulos

1996

Proc. Natl. Acad. Sci. U.S.A.

218

190

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“…1A, marker). In addition, circular RNAs have been demonstrated to be more stable to nucleolytic degradation than their linear counterparts, and the functional properties of many RNAs are not significantly altered as a result of the ligation (15)(16)(17).…”

Section: Resultsmentioning

confidence: 99%

A Cis-acting A-U Sequence Element Induces Kinetoplastid U-insertions

Brown

Burbach

McKenzie

et al. 1999

Journal of Biological Chemistry

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A 34-nucleotide A-U sequence located immediately upstream of the editing sites of the Leishmania tarentolae cytochrome b mRNA induces a mitochondrial extract to insert U nucleotides independent of guide RNA. Insertions are localized to positions immediately 5 and 3 of the A-U sequence. When placed within an unedited mammalian transcript, the A-U sequence is sufficient to induce U-insertions. The sequence has a high degree of similarity with the templating nucleotides of a cytochrome b guide RNA and with a sequence adjacent to the editing sites in ND7 mRNA, the other characterized kinetoplastid mRNA supporting guide RNA-independent U-insertions. At least one protein specifically interacts with the A-U sequence. The reaction is consistent with a mechanism proposed for guide RNA-directed editing.

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“…However, circular, single-exon-containing RNA molecules have also been observed during the expression of the ETS-1 gene (1). In addition, it has been shown that circular RNA molecules can be readily generated in vitro from both linear or circular yeast pre-mRNAs (18,19).…”

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confidence: 99%

Exon Skipping and Circular RNA Formation in Transcripts of the Human Cytochrome P-450 2C18 Gene in Epidermis and of the Rat Androgen Binding Protein Gene in Testis

Zaphiropoulos

1997

Molecular and Cellular Biology

159

139

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The cytochrome P-450 2C18 gene was found by reverse transcription-PCR to represent the most abundantly expressed gene of the P-450 2C subfamily in human epidermis. However, in addition to the canonical mRNA of nine exons, transcripts that have skipped exon 4 or 5, exons 4, 5, and 6, or exons 4, 5, 6, and 7 were also identified in this tissue. Remarkably, circular RNA transcripts synthesized by the joining of the donor and acceptor splice sites of the same exon were detected in human epidermis for exons 4 and 5. Moreover, molecules composed of exons 4, 5, and 6 with the donor splice site of exon 6 joined to the acceptor splice site of exon 4 or composed of exons 4, 5, 6, and 7 with the donor splice site of exon 7 joined to the acceptor splice site of exon 4 were also found to be present in this tissue. In rat testis, a similar analysis allowed the detection of a circular RNA molecule composed of exons 6 and 7 of the androgen binding protein (ABP) gene, with the donor splice site of exon 7 joined to the acceptor splice site of exon 6, and of an ABP mRNA which had skipped exons 6 and 7. These results apparently substantiate the hypothesis that alternative pre-mRNA splicing has the potential to generate not only mRNAs that lack one or more exons but also circular RNA molecules that are composed of the exons that are skipped. However, additional 2C18 circular species containing various combinations of exons were also detected in human epidermis, and an exon 6-skipped ABP mRNA molecule was identified in rat testis. This observation is interpreted as indicative that at low frequency, numerous circular RNA formation and exon skipping events may occur, allowing the joining of a variety of different combinations of exons. Moreover, the relative stability of these molecules is apparently the key factor that determines the relative ease of their detection.

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Splicing of a circular yeast pre-mRNAin vitro

Cited by 10 publications

References 40 publications

Circular RNAs from transcripts of the rat cytochrome P450 2C24 gene: correlation with exon skipping.

Circular RNAs from transcripts of the rat cytochrome P450 2C24 gene: correlation with exon skipping.

A Cis-acting A-U Sequence Element Induces Kinetoplastid U-insertions

Exon Skipping and Circular RNA Formation in Transcripts of the Human Cytochrome P-450 2C18 Gene in Epidermis and of the Rat Androgen Binding Protein Gene in Testis

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