Splicing factor SNRPA associated with microvascular invasion promotes hepatocellular carcinoma metastasis through activating NOTCH1/Snail pathway and is mediated by circSEC62/miR‐625‐5p axis

Abstract

Microvascular invasion (MVI) is a crucial risk factor related to the metastasis of hepatocellular carcinoma (HCC), but the underlying mechanisms remain to be revealed. Characterizing the inherent mechanisms of MVI may aid in the development of effective treatment strategies to improve the prognosis of HCC patients with metastasis. Through the Gene Expression Omnibus (GEO) database, we identified that small nuclear ribonucleoprotein polypeptide A (SNRPA) was related to MVI in HCC. SNRPA was overexpressed in MVI‐HCC and correlated with poor patient survival. Mechanistically, SNRPA promoted the epithelial‐mesenchymal transition (EMT)‐like process for HCC cells to accelerate metastasis by activating the NOTCH1/Snail pathway in vitro and in vivo. Importantly, circSEC62 upregulated SNRPA expression in HCC cells via miR‐625‐5p sponging. Taking these results together, our study identified a novel regulatory mechanism among SNRPA, miR‐625‐5p, circSEC62 and the NOTCH1/Snail pathway in HCC, which promoted metastasis of HCC and may provide effective suggestions for improving the prognosis of HCC patients with metastasis.

Spleen tyrosine kinase facilitates the progression of papillary thyroid cancer regulated by the hsa_circ_0006417/miR‐377‐3p axis

“…Paraffin blocks of PTC and normal tissue were deparaffinized and hydrated, followed by treatment with citrate buffer as previously described 40 . Sections were treated with Anti‐SYK antibody (1:200, ab155187, Abcam, USA), anti‐p‐SYK (1:200, ab62338, Abcam, USA), and anti‐Galectin‐3 (1:150, ab2785, Abcam, USA) for 1 h at 37°C.…”

“…Paraffin blocks of PTC and normal tissue were deparaffinized and hydrated, followed by treatment with citrate buffer as previously described. 40 Sections were treated with Anti-SYK antibody (1:200, ab155187, Abcam, USA), anti-p-SYK (1:200, ab62338, Abcam, USA), and anti-Galectin-3 (1:150, ab2785, Abcam, USA) for 1 h at 37 C. Secondary antibody was added for 30 min incubation, followed by DAB staining for protein signal detection. IHC scores were computed by multiplying staining intensity (negative, 0; weak, 1; moderate, 2; or strong, 3) and percentage of positive cells (0; <25%, 1; 25%-50%, 2; 50%-75%, 3; >75%, 4;).…”

Nanoparticles Mediated circROBO1 Silencing to Inhibit Hepatocellular Carcinoma Progression by Modulating miR-130a-5p/CCNT2 Axis

“…4 For instance, circSEC62 promoted SNRPA levels by silencing miR-625-5p and affected HCC microvascular invasion. 10 Since ROBO1 was aberrant expressed in various cancers, [11][12][13] but the function of ROBO1 derived circRNAs in cancers still remained unclear. Among 40 circRNAs derived from parental gene ROBO1, only hsa_circ_0124696 (back splicing exon 5-8 of ROBO1) was identified to regulate the liver metastasis of breast cancer.…”

Hsa_circ_0008500 inhibits apoptosis of adipose‐derived stem cells under high glucose through hsa‐miR‐1273h‐5p/ELK1 axis

“…CircRNAs are derived from a non‐canonical form of variable splicing, where the splice donor spot of one exon is ligated to the splice acceptor spot of an up stream exon 11 . Diverse from linearity RNA, circRNAs produce a continuous steady loop framework in the absence of 5′cap or 3′tail, which promotes its resistant feature to exonuclease digestion 12 . The biofunction of circRNAs is versatile, changing greatly from the location, binding spots and behavior patterns of circRNAs 13 .…”

“…absence of 5 0 cap or 3 0 tail, which promotes its resistant feature to exonuclease digestion. 12 The biofunction of circRNAs is versatile, changing greatly from the location, binding spots and behavior patterns of circRNAs. 13 Andreeva 14 reported that circRNAs existed extensively in cellular tissue of plants and animals.…”