Splicing Factor ChIP and ChRIP: Detection of Splicing and Splicing Factors at Genes by Chromatin Immunoprecipitation

Sapra, Aparna K.; Oesterreich, Fernando Carrillo; Pabiś, Marta; Listerman, Imke; Bieberstein, Nicole; Neugebauer, Karla M.

doi:10.1002/9783527636778.ch39

Cited by 2 publications

(1 citation statement)

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“…Some splicing factors are involved in DNA repair and replication of the telomeric regions, as well as maintenance of the genome stability through regulation of appearance and resolution of R-loops formed between nascent transcripts and complementary DNA strand during transcription [29][30][31][32]. Spliceosome proteins combine the functions of genome stability regulators and splicing participants, which is made possible by their interaction with both chromatin [23,33] and transcribed mRNAs. Seemingly, this allows for efficient coordination and rapid switching between the functions depending on the immediate needs of the cell.…”

Section: Non-canonical Functions Of Spliceosome Componentsmentioning

confidence: 99%

Non-canonical functions of spliceosome components in cancer progression

et al. 2023

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Dysregulation of pre-mRNA splicing is a common hallmark of cancer cells and it is associated with altered expression, localization, and mutations of the components of the splicing machinery. In the last few years, it has been elucidated that spliceosome components can also influence cellular processes in a splicing-independent manner. Here, we analyze open source data to understand the effect of the knockdown of splicing factors in human cells on the expression and splicing of genes relevant to cell proliferation, migration, cell cycle regulation, DNA repair, and cell death. We supplement this information with a comprehensive literature review of non-canonical functions of splicing factors linked to cancer progression. We also specifically discuss the involvement of splicing factors in intercellular communication and known autoregulatory mechanisms in restoring their levels in cells. Finally, we discuss strategies to target components of the spliceosome machinery that are promising for anticancer therapy. Altogether, this review greatly expands understanding of the role of spliceosome proteins in cancer progression.

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