Spliced human endogenous retroviral HERV-H env transcripts in T-cell leukaemia cell lines and normal leukocytes: alternative splicing pattern of HERV-H transcripts.

Lindeskog, Mats; Blomberg, Jonas

doi:10.1099/0022-1317-78-10-2575

Cited by 30 publications

(26 citation statements)

References 40 publications

(38 reference statements)

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“…Additionally, we observed upregulation of nonfunctional transcripts derived from mariner 2 transposon (HSMAR2) remnants and human endogenous retrovirus H (HERV-H) sequences that have acquired premature termination codons (PTCs) during evolution. Previous studies [15][16][17] and our analyses of expressed-sequence tags showed that at least some HSMAR2 and HERV-H transcripts undergo splicing. Accordingly, we observed B1.0-kb transcripts corresponding to the size of the spliced HERV-H message (Fig.…”

Section: E T T E R Smentioning

confidence: 62%

Nonsense surveillance regulates expression of diverse classes of mammalian transcripts and mutes genomic noise

et al. 2004

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Section: E T T E R Smentioning

confidence: 62%

Nonsense surveillance regulates expression of diverse classes of mammalian transcripts and mutes genomic noise

et al. 2004

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“…Further, polymorphism and epigenetic events such as imprinting could lead to both inter-and intraindividual variation in HERV expression. An interindividual variation in RNA expression has been shown for several HERVs (7,42,48,85).…”

Section: Discussionmentioning

confidence: 99%

ERV3 and Related Sequences in Humans: Structure and RNA Expression

Andersson¹,

Yun²,

Sperber

et al. 2005

J Virol

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The ERV3 locus at chromosome 7q11 is a much studied human endogenous retroviral (HERV) sequence, owing to an env open reading frame (ORF) and placental RNA and protein expression. An analysis of the human genome demonstrated that ERV3 is one of a group of 41 highly related elements (ERV3-like HERVs) which use proline, isoleucine, or arginine tRNA in their primer binding sites. In addition to elements closely related to ERV3, the group included the previously known retinoic acid-inducible element, RRHERVI, also referred to as HERV15, but was separate from the related HERV-E elements. The ERV3-like elements are defective. The only element with an ORF among gag, pro, pol, and env genes was the env ORF of the original ERV3 locus. A search in dbEST revealed ERV3 RNA expression in placenta, skin, carcinoid tumor, and adrenal glands. Expression was also studied with newly developed real-time quantitative PCRs (QPCR) of ERV3 and HERV-E(4-1) env sequences. Results from a novel histone 3.3 RNA QPCR result served as the expression control. QPCR results for ERV3 were compatible with previously published results, with a stronger expression in adrenal gland and placenta than in 15 other human tissues. The expression of the envelope (env) of ERV3 at chromosome 7q11 was also studied by using stringent in situ hybridization. Expression was found in corpus luteum, testis, adrenal gland, Hassal's bodies in thymus, brown fat, pituitary gland, and epithelium of the lung. We conclude that ERV3 env is most strongly expressed in adrenal and sebaceous glands as well as in placenta.

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“…The annotated genes are distributed among the SDs on chromosomes 2, 3, 7, 10, 11, and 13. The described functions of the non-OR genes vary widely and include a hypothetical zinc finger gene, an oxytocin receptor, a HERV-H protein, and an A-kinase anchoring protein (AKAP)-binding sperm roporin (Kimura et al 1992;Lindeskog and Blomberg 1997;Carr et al 2001). PC3-96, an autophagy-like protein, is the only non-OR gene that lies within 50 kb of a 7E gene (∼9 kb 5Ј of the 7E gene at 3.125919193).…”

Section: Org Downloaded Frommentioning

confidence: 99%

Complex Evolution of 7E Olfactory Receptor Genes in Segmental Duplications

Newman

Trask²

2003

Genome Res.

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Large segmental duplications (SDs) constitute at least 3.6% of the human genome and have increased its size, complexity, and diversity. SDs can mediate ectopic sequence exchange resulting in gross chromosomal rearrangements that could contribute to speciation and disease. We have identified and evaluated a subset of human SDs that harbor an 88-member subfamily of olfactory receptor (OR)-like genes called the 7Es. At least 92% of these genes appear to be pseudogenes when compared to other OR genes. The 7E-containing SDs (7E SDs) have duplicated to at least 35 regions of the genome via intra-and interchromosomal duplication events. In contrast to many human SDs, the 7E SDs are not biased towards pericentromeric or subtelomeric regions. We find evidence for gene conversion among 7E genes and larger sequence exchange between 7E SDs, supporting the hypothesis that long, highly similar stretches of DNA facilitate ectopic interactions. The complex structure and history of the 7E SDs necessitates extension of the current model of large-scale DNA duplication. Despite their appearance as pseudogenes, some 7E genes exhibit a signature of purifying selection, and at least one 7E gene is expressed.

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Spliced human endogenous retroviral HERV-H env transcripts in T-cell leukaemia cell lines and normal leukocytes: alternative splicing pattern of HERV-H transcripts.

Cited by 30 publications

References 40 publications

Nonsense surveillance regulates expression of diverse classes of mammalian transcripts and mutes genomic noise

Nonsense surveillance regulates expression of diverse classes of mammalian transcripts and mutes genomic noise

ERV3 and Related Sequences in Humans: Structure and RNA Expression

Complex Evolution of 7E Olfactory Receptor Genes in Segmental Duplications

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