Splenocyte Plaque Assay for the Detection of Murine Leukemia Virus

Melief, C. J. M.; Datta, Somnath; Louie, Stephen; Johnson, Stephen M.; Melief, Mary; Schwartz, Robert S.

doi:10.3181/00379727-149-38946

Cited by 27 publications

(13 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The UV-XC procedure described by Rowe and Pincus (12) was modified for testing spleen cell suspensions as an infectious center assay. This technique has been described in detail previously (9). NIH 3T3 cells and BALB/c mouse embryo fibroblasts were used to detect Nand B-tropic viruses, respectively.…”

mentioning

confidence: 99%

Genetic studies of autoimmunity and retrovirus expression in crosses of New Zealand black mice I. Xenotropic virus.

Datta

Manny

Andrzejewski

et al. 1978

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Type C RNA viruses (retroviruses) have been implicated in the autoimmune disease of New Zealand Black (NZB) 1 mice (1, 2). However, the evidence for this is only circumstantial because the retroviruses isolated from NZB mice are xenotropic in host range; this prohibits a direct test of their pathogenicity in mice by, for example, inoculation of filtrates (3, 4). Nevertheless, the relationship between autoimmunization and xenotropic viruses c&n be ascertained by genetic tests because the genes of these agents are an integral part of cellular chromosomes (5) and their expression in NZB mice is governed by two autosomal dominant genes (6, 7). In this paper we describe results of genetic experiments designed to test the hypothesis that expression of xenotropic virus is required for the development of autoimmune disease. The following paper (8) deals with the relationship between autoimmune disease in NZB mice and expression of the major envelope glycoprotein of the virus, gp70. Materials and MethodsMice. NZB, SWR, C57BL/6, B10.A, and AKR mice were obtained from The Jackson Laboratory, Bar Harbor, Maine. Reciprocal crosses were made to produce FI and backcross progeny mice. Because the maternal direction of the cross did not affect the results, all data for a given cross were pooled and presented by a single nomenclature: F~ for (SWR × NZB)F~; (F~ × SWR) for backcross to SWR, and (F~ x NZB) for backcross to NZB. The mice were 11-24 mo old when tested.Retrovirus Assays XI~.NOTROPIC VIRUS. Spleen cell suspensions were prepared and serially diluted as described previously (6, 7, 9). They were assayed by cocultivation on monolayers of the feline 81 cell line

show abstract

mentioning

confidence: 99%

Genetic studies of autoimmunity and retrovirus expression in crosses of New Zealand black mice I. Xenotropic virus.

Datta

Manny

Andrzejewski

et al. 1978

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…Cells from different populations of the four cell types were treated with mitomycin C (Kawashima et al, 1976) and inoculated at different dilutions on to DEAE-dextran-treated mink or mouse indicator fibroblast cultures (Vogt, 1967) for detection of virus activity (Melief et al, 1975). The assay was linear with cell dilution.…”

Section: Methodsmentioning

confidence: 99%

“…NIH, SC-1 and Balb/c mouse embryo cells were used to detect ecotropic virus (Rowe et al, 1970) or virus-producing cells as infectious eentres (Melief et al, 1975).…”

Section: Methodsmentioning

confidence: 99%

Biological Characteristics of Type C Viruses Isolated from Different Friend Erythroleukaemic Cells

Vinton

Woytowicz

Levy

1982

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYWe have examined the expression of type C RNA viruses in different Friend erythroleukaemic cell types, distinguished on the basis of increasingly malignant characteristics, which arise during the ageing of mice inoculated with the polycythaemic Friend virus complex. Most early appearing Friend cells (type I) expressed ecotropic virus, but cells of later malignant types showed decreased and variable expression. In general, the more malignant cells released less ecotropic virus. Xenotropic virus was detected in low numbers from type I, II, and IV cells. Two viruses were cloned from type II tumour cells: a xenotropic virus (II clone l) and an N-tropic ecotropic virus (II clone 2). No pathogenic activity was found when II clone 1 was inoculated into newborn and adult DBA/2J and NIH/Swiss mice observed for up to 20 months, whereas II clone 2 caused a rapid anaemic erythroleukaemia in both N-and B-type newborn mouse strains. It caused a similar form of leukaemia in susceptible N-type adult mice, but at a lower frequency and with a longer latency (usually >5 months). This finding demonstrated a lack of NB restriction in newborn mice. The virus was much less active in DBA/2J mice from which it had been originally cloned; it also appeared to cause lymphoma or to shorten the latency of spontaneous lymphoma in DBA/2J mice.

show abstract

“…After two passages, the latter were tested for the presence of infectious virus using the XC test (Rowe et aL, 1970). This procedure allowed the detection of low virusyielder cells from individual granulocyte-macrophage colonies that would not score as infectious centres in the assay described by Melief et al (1975).…”

mentioning

confidence: 99%

“…Singlecell suspensions were prepared from the liver of individual embryos or from pooled spleens of embryos of the same litter, and virus production was studied using two methods. The cells were plated onto mouse NB-type indicator cells (3T3 F1 clone 5D) (Gisselbrecht et aL, 1974) and the number of virus-producing cells was determined using the infectious centre assay developed by Melief et al (1975). In a parallel assay, granulocyte-macrophage progenitor ceils (or colonyforming cells: GM-CFC) were stimulated to differentiate in vitro by plating the cells in semisolid medium containing horse serum and granulocyte-macrophage colony-stimulating factor (GM-CSF) from mouse lung-conditioned medium (Sheridan & Metcalf, 1973).…”

mentioning

confidence: 99%

Expression of Ecotropic Murine Leukaemia Virus in Haemopoietic Cells of AKR Mice during the Embryonic and Neonatal Period

Godard¹,

Augery²,

Jasmin³

1983

Journal of General Virology

View full text Add to dashboard Cite

Splenocyte Plaque Assay for the Detection of Murine Leukemia Virus

Cited by 27 publications

References 0 publications

Genetic studies of autoimmunity and retrovirus expression in crosses of New Zealand black mice I. Xenotropic virus.

Genetic studies of autoimmunity and retrovirus expression in crosses of New Zealand black mice I. Xenotropic virus.

Biological Characteristics of Type C Viruses Isolated from Different Friend Erythroleukaemic Cells

Expression of Ecotropic Murine Leukaemia Virus in Haemopoietic Cells of AKR Mice during the Embryonic and Neonatal Period

Contact Info

Product

Resources

About