Splenectomy fails to attenuate immuno-hematologic changes after rodent vertical sleeve gastrectomy

Himel, Alexandra R; Taylor, Erin B.; Phillips, Charles L.; Welch, Bradley A.; Spann, Redin A.; Bandyopadhyay, Sibali; Grayson, Bernadette E.

doi:10.1177/1535370219857991

Cited by 5 publications

(4 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10E). Consistent with prior literature, CD45 + CD3 + CD4 + were considered Helper T cells and CD45 + CD3 + CD4 + CD25 + were considered regulatory T cells (Tregs) [77,78] (Fig. 10F).…”

Section: In Utero Injury Induces Long-term Changes In Peripheral and Central Immune Cell Populationssupporting

confidence: 80%

Sustained peripheral immune hyper-reactivity (SPIHR): an enduring biomarker of altered inflammatory responses in adult rats after perinatal brain injury

Kitase

Chin

Ramachandra

et al. 2021

J Neuroinflammation

View full text Add to dashboard Cite

Background Chorioamnionitis (CHORIO) is a principal risk factor for preterm birth and is the most common pathological abnormality found in the placentae of preterm infants. CHORIO has a multitude of effects on the maternal–placental–fetal axis including profound inflammation. Cumulatively, these changes trigger injury in the developing immune and central nervous systems, thereby increasing susceptibility to chronic sequelae later in life. Despite this and reports of neural–immune changes in children with cerebral palsy, the extent and chronicity of the peripheral immune and neuroinflammatory changes secondary to CHORIO has not been fully characterized. Methods We examined the persistence and time course of peripheral immune hyper-reactivity in an established and translational model of perinatal brain injury (PBI) secondary to CHORIO. Pregnant Sprague–Dawley rats underwent laparotomy on embryonic day 18 (E18, preterm equivalent). Uterine arteries were occluded for 60 min, followed by intra-amniotic injection of lipopolysaccharide (LPS). Serum and peripheral blood mononuclear cells (PBMCs) were collected at young adult (postnatal day P60) and middle-aged equivalents (P120). Serum and PBMCs secretome chemokines and cytokines were assayed using multiplex electrochemiluminescent immunoassay. Multiparameter flow cytometry was performed to interrogate immune cell populations. Results Serum levels of interleukin-1β (IL-1β), IL-5, IL-6, C–X–C Motif Chemokine Ligand 1 (CXCL1), tumor necrosis factor-α (TNF-α), and C–C motif chemokine ligand 2/monocyte chemoattractant protein-1 (CCL2/MCP-1) were significantly higher in CHORIO animals compared to sham controls at P60. Notably, CHORIO PBMCs were primed. Specifically, they were hyper-reactive and secreted more inflammatory mediators both at baseline and when stimulated in vitro. While serum levels of cytokines normalized by P120, PBMCs remained primed, and hyper-reactive with a robust pro-inflammatory secretome concomitant with a persistent change in multiple T cell populations in CHORIO animals. Conclusions The data indicate that an in utero inflammatory insult leads to neural–immune changes that persist through adulthood, thereby conferring vulnerability to brain and immune system injury throughout the lifespan. This unique molecular and cellular immune signature including sustained peripheral immune hyper-reactivity (SPIHR) and immune cell priming may be a viable biomarker of altered inflammatory responses following in utero insults and advances our understanding of the neuroinflammatory cascade that leads to perinatal brain injury and later neurodevelopmental disorders, including cerebral palsy.

show abstract

“…10E). Consistent with prior literature, CD45 + CD3 + CD4 + were considered Helper T cells and CD45 + CD3 + CD4 + CD25 + were considered regulatory T cells (Tregs) [77,78] (Fig. 10F).…”

Section: In Utero Injury Induces Long-term Changes In Peripheral and Central Immune Cell Populationssupporting

confidence: 80%

Sustained peripheral immune hyper-reactivity (SPIHR): an enduring biomarker of altered inflammatory responses in adult rats after perinatal brain injury

Kitase

Chin

Ramachandra

et al. 2021

J Neuroinflammation

View full text Add to dashboard Cite

show abstract

“…This polarization shift may be related to weight lossinduced decreases in the expression of molecules involved in macrophage chemotaxis and relief of the tissue hypoxia commonly found in obese patients [270]. Multiple studies have also described tissue-specific shifts in neutrophil populations, with increased neutrophils found in splenic and adipose tissue after bariatric surgery [265,[271][272][273]. Considerable work has been done to examine the effects of obesity, weight loss, and bariatric surgery on the B cell compartment to restore obesity-associated dysfunction [272][273][274][275][276][277].…”

Section: Bariatric Surgery and The Immune Systemmentioning

confidence: 98%

“…Multiple studies have also described tissue-specific shifts in neutrophil populations, with increased neutrophils found in splenic and adipose tissue after bariatric surgery [265,[271][272][273]. Considerable work has been done to examine the effects of obesity, weight loss, and bariatric surgery on the B cell compartment to restore obesity-associated dysfunction [272][273][274][275][276][277]. With regard to cancer risk, studies must understand how bariatric surgery will impact specific immune subtypes, which depots (circulating, WAT, breast/mammary gland, or other tissues) will be affected, and how long these changes will persist after surgery.…”

Section: Bariatric Surgery and The Immune Systemmentioning

confidence: 99%

The role of obesity and bariatric surgery-induced weight loss in breast cancer

Bohm

Sipe

Pye

et al. 2022

Cancer Metastasis Rev

View full text Add to dashboard Cite

Obesity is a complex metabolic condition considered a worldwide public health crisis, and a deeper mechanistic understanding of obesity-associated diseases is urgently needed. Obesity comorbidities include many associated cancers and are estimated to account for 20% of female cancer deaths in the USA. Breast cancer, in particular, is associated with obesity and is the focus of this review. The exact causal links between obesity and breast cancer remain unclear. Still, interactions have emerged between body mass index, tumor molecular subtype, genetic background, and environmental factors that strongly suggest obesity influences the risk and progression of certain breast cancers. Supportive preclinical research uses various diet-induced obesity models to demonstrate that weight loss, via dietary interventions or changes in energy expenditure, reduces the onset or progression of breast cancers. Ongoing and future studies are now aimed at elucidating the underpinning mechanisms behind weight-loss-driven observations to improve therapy and outcomes in patients with breast cancer and reduce risk. This review aims to summarize the rapidly emerging literature on obesity and weight loss strategies with a focused discussion of bariatric surgery in both clinical and preclinical studies detailing the complex interactions between metabolism, immune response, and immunotherapy in the setting of obesity and breast cancer.

show abstract

“…Further, VSG offspring exhibit profound changes to their immune systems as a result of being born to dams that have lost weight through bariatric means [ 27 ]. This is not surprising, given the profound impact of VSG on the lymphoid organs and immune system following VSG [ 22 , 28 ]. Further, as in the current paper, we previously reported metabolic and immune problems in adult offspring [ 22 , 23 , 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Altered Estrous Cyclicity and Feeding Neurocircuitry, but Not Cardiovascular Indices in Female Offspring from Dams with Previous Vertical Sleeve Gastrectomy Surgery

Johnson,

Welch,

Aravindan

et al. 2023

Brain Sciences

View full text Add to dashboard Cite

Metabolic syndrome (MetS), which includes obesity, diabetes, hypertension, hyperlipidemia, and fatty-liver disease, affects more than two-thirds of the U.S. population. Surgical weight loss has been popularized in the last several decades as a means to produce significant weight loss and improvements in the comorbidities of MetS. Women are by far the most common recipients of these surgeries (more than 85%). Women of childbearing age are very likely to pursue surgical weight loss to improve their reproductive function and fertility for childbearing purposes. Significant research using pre-clinical models from our laboratory and clinical data from around the world suggest that surgical weight loss before pregnancy may have negative consequences for offspring. The present study investigates the metabolic endpoints in female-rodent offspring born to dams who had previously received vertical sleeve gastrectomy (VSG) before pregnancy. Comparisons were made to offspring from lean and obese dams. In the adult offspring of either maternal VSG or sham surgery, no differences in body weight, body fat, or lean body mass between groups were identified. The blood pressure measured in a subset of female offspring showed no differences between the VSG and the sham groups. Estrus cyclicity measured by lavage on serial days showed altered cycles in the VSG offspring compared to the controls. For animals that had previously only been exposed to chow, rats were fasted overnight and then given a 1 g meal of either chow or a novel high-fat diet (HFD). The animals were euthanized and paraformaldehyde (PFA)-perfused to perform brain immunohistochemistry for c-Fos, an immediate–early gene activated by novel stimuli. In the VSG rats exposed to either the chow or the HFD meal, the c-Fos-activated cells were significantly blunted in the nucleus of the solitary tract (p < 0.05), the paraventricular nucleus of the hypothalamus (PVN) (p < 0.05), and the dorsal medial nucleus of the hypothalamus (DMH) (p < 0.05) in comparison to the sham controls. These data suggest that the hypothalamic wiring within the brain that controls the response to nutrients and reproductive function was significantly altered in the VSG offspring compared to the offspring of the dams that did not receive weight-loss surgery.

show abstract

Splenectomy fails to attenuate immuno-hematologic changes after rodent vertical sleeve gastrectomy

Cited by 5 publications

References 41 publications

Sustained peripheral immune hyper-reactivity (SPIHR): an enduring biomarker of altered inflammatory responses in adult rats after perinatal brain injury

Sustained peripheral immune hyper-reactivity (SPIHR): an enduring biomarker of altered inflammatory responses in adult rats after perinatal brain injury

The role of obesity and bariatric surgery-induced weight loss in breast cancer

Altered Estrous Cyclicity and Feeding Neurocircuitry, but Not Cardiovascular Indices in Female Offspring from Dams with Previous Vertical Sleeve Gastrectomy Surgery

Contact Info

Product

Resources

About