Spleen Histopathological Study of Mice (Mus musculus) Infected with Plasmodium berghei After Treatment with Malacca Leaves extract (Phyllantus emblica)

Abstract

This study aimed to evaluate the effect of ethanolic extract of malacca leaves (Phyllantus emblica) on histopathology of mice (Mus musculus) spleen infected with Plasmodium berghei (P. berghei). This study used 15 male Balb/c strain mice aged 2 months, allotted into 5 treatment groups. The mice in group 1 (P0) were infected with P. berghei without administration of Malacca leaves extract, while the mice in group 2 (P1) were infected with P. berghei and treated with 5 mg/kg BW of chloroquine. The mice in group 3 (P2), 4 (P3), and 5 (P4) were infected with P. berghei and treated with Malacca leaves extract at doses of 100, 300, and 600 mg/kg BW, respectively. At day 7 th , all mice were sacrificed, then the spleen were collected for histopathological examination. The observation was carried out on the hemosiderin accumulation, lymphocyte depletion, hemorrhage, and megakaryocyte cells. Data were analyzed using one way analysis of variance (ANOVA). The results showed that the amount of hemosiderin, lymphocyte depletion, and hemorrhage decreased in mice given Malacca leaves extract compared to those in the negative control. In conclusion, the administration of ethanolic extract of malacca leaves reduce the damage of mice spleen due to P. berghei infection