Spirulina phycocyanin induces differential protein expression and apoptosis in SKOV-3 cells

Pan, Ruowang; Lu, Rongmao; Zhang, Ying; Zhu, Mei; Zhu, Wen; Yang, Rongrong; Zhang, Enyong; Ying, Jun; Xu, Teng; Yi, Huiguang; Li, Jinsong; Zhou, Li; Xu, Zuyuan; Li, Peizhen; Bao, Qiyu

doi:10.1016/j.ijbiomac.2015.09.039

Cited by 34 publications

(25 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study was consistent and extended previous findings on the effects of phycocyanin as an antiapoptosis. Phycocyanin apoptosis modulation was occurs through mitochondrial pathway [16][17][18][19]. To decrease the expression of IL-17 is found only in the dosage of phycocyanin doses of 40 and 80 ng.…”

Section: Discussionmentioning

confidence: 99%

Induction of interleukin-6 trigger an apoptosis through IL-17 and stat3 pathway that alleviated by phycocyanin treatment

Haryanti¹,

Gondo²

2018

J.Ilm.Kedokt. Wijaya Kusuma

View full text Add to dashboard Cite

AbstrakPenelitian ini bertujuan untuk menganalisis dosis optimal induksi IL-6 pada tikus hamil yang dapat memicu peningkatan tekanan arteri dan protein sebagai dua gejala preeklampsia. Sebanyak 25 tikus hamil dibagi menjadi 5 kelompok, yaitu tikus hamil kelompok kontrol (tanpa induksi IL-6), 1 kelompok tikus hamil diberi induksi IL-6 dalam dosis 1,25 ng / hari, 1 kelompok tikus hamil diberi induksi IL-6 dosis 2,5 ng/ hari, 1 kelompok tikus hamil diberi induksi IL-6 dosis 5 ng / hari, dan kelompok tikus hamil diberi induksi IL-6 dosis 10 ng / hari. Induksi IL-6 dilakukan pada hari kesepuluh kehamilan selama 5 hari. Ekspresi caspase-3, IL-17, dan STAT3 dianalisis dengan mikroskop confocal. Ekspresi caspase-3, IL-17 dan STAT3 secara signifikan lebih tinggi pada kelompok preeclampsia dibandingkan kelompok kontrol (p <0,05). Ekspresi caspase-3 menurun secara signifikan pada semua kelompok yang diobati dengan phycocyanin dibandingkan kelompok preeklampsia (p <0,05), namun belum dapat mencapai ekspresi yang sebanding dengan kelompok kontrol (p <0,05). Ekspresi IL-17 menurun secara signifikan pada kelompok tersebut dengan pemberian dua dosis tertinggi phycocyanin dibandingkan dengan kelompok preeklamsia (p <0,05). Ekspresi STAT3 menurun secara signifikan pada semua kelompok yang diberi phycocyanin dibandingkan kelompok preeklampsia (p <0,05), mencapai ekspresi yang sebanding dengan kelompok kontrol pada kelompok yang menerima phycocyanin pada dosis 10 dan 20 ng (p> 0,05). Kesimpulannya, IL-6 pada tikus hamil mampu meningkatkan apoptosis melalui jalur IL-17 dan STAT. Penghambatan apoptosis akibat pengobatan phycoyanin tidak hanya melibatkan pembentukan IL-17, data ini ditemukan dalam dosis phycocyanin 10 dan 20 ng. AbstractThis study was to analyze the optimum dose of IL-6 induction in pregnant rats that could trigger an increase in mean arterial pressure and protein as two symptoms of preeclampsia. A total of 25 pregnant rats was divided into 5 groups, including pregnant rats the control group (without induction of IL-6), a group of pregnant rats were given an induction in IL-6 doses of 1.25 ng/day, a group of pregnant rats given doses of IL-6 induction 2.5 ng/day, a group of pregnant rats were given an induction of IL-6 doses of 5 ng/day, and groups of pregnant rats were given an induction in IL-6 doses of 10 ng/day. Induction of IL-6 was performed on the tenth day of gestation for 5 days. The expression of caspase-3, IL-17, and STAT3 were analyzed by confocal laser scanning microscopy. The expression of caspase-3, IL-17 and STAT3 was significantly higher in preeclampsia group than the control group (p<0.05). The expression of caspase-3 decreased significantly in all groups were treated by phycocyanin compared to the preeclamptic group (p<0.05), but has not been able to reach expression comparable to the control group (p<0.05). Expression of IL-17 decreased significantly in the group given the two highest doses of phycocyanin compared to the preeclampsia group (p<0.05). STAT3 expression decreased significantly in ISSN 1...

show abstract

Section: Discussionmentioning

confidence: 99%

Induction of interleukin-6 trigger an apoptosis through IL-17 and stat3 pathway that alleviated by phycocyanin treatment

Haryanti¹,

Gondo²

2018

J.Ilm.Kedokt. Wijaya Kusuma

View full text Add to dashboard Cite

show abstract

“…Molecular mechanisms of C-PC-induced apoptosis have not been completely elucidated and possible mechanisms such as inhibiting COX-2 activity, reducing the expression of Bcl-2, enhancing the expression of CD59, and activating proteins from the cysteine protease family have been suggested [52]. Recently, Pan et al [53] reported that C-PC may regulate the expressions of many kinds of proteins in SKOV-3 ovarian carcinoma cells to affect the process of apoptosis, oxidative stress, lipid, and ion transportation and finally inhibit the growth of tumour cells. Although the concentrations used may have been too low to show an effect after administration alone, it is not excluded that this was still enough to add to the effect of betaine by influencing one of the above mentioned pathways.…”

Section: Discussionmentioning

confidence: 99%

Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 CellsIn VitroandIn Vivo

Bingula

Dupuis

Pichon

et al. 2016

Journal of Oncology

View full text Add to dashboard Cite

We investigated the effects of betaine, C-phycocyanin (C-PC), and their combined use on the growth of A549 lung cancer both in vitro and in vivo. When cells were coincubated with betaine and C-PC, an up to 60% decrease in viability was observed which is significant compared to betaine (50%) or C-PC treatment alone (no decrease). Combined treatment reduced the stimulation of NF-κB expression by TNF-α and increased the amount of the proapoptotic p38 MAPK. Interestingly, combined treatment induced a cell cycle arrest in G2/M phase for ~60% of cells. In vivo studies were performed in pathogen-free male nude rats injected with A549 cells in their right flank. Their daily food was supplemented with either betaine, C-PC, both, or neither. Compared to the control group, tumour weights and volumes were significantly reduced in either betaine- or C-PC-treated groups and no additional decrease was obtained with the combined treatment. This data indicates that C-PC and betaine alone may efficiently inhibit tumour growth in rats. The synergistic activity of betaine and C-PC on A549 cells growth observed in vitro remains to be further confirmed in vivo. The reason behind the nature of their interaction is yet to be sought.

show abstract

“…Pan et al ( 2015 ) showed the inhibitory effect of phycocyanin on the proliferation of the ovarian cancer SKOV-3 cell line. Phycocyanin also induced the apoptosis by the mitochondrial pathway.…”

Section: Arthrospira Human Health and Medicine—the Multipmentioning

confidence: 99%

Edible Cyanobacterial Genus Arthrospira: Actual State of the Art in Cultivation Methods, Genetics, and Application in Medicine

et al. 2017

View full text Add to dashboard Cite

The cyanobacterial genus Arthrospira appears very conserved and has been divided into five main genetic clusters on the basis of molecular taxonomy markers. Genetic studies of seven Arthrospira strains, including genome sequencing, have enabled a better understanding of those photosynthetic prokaryotes. Even though genetic manipulations have not yet been performed with success, many genomic and proteomic features such as stress adaptation, nitrogen fixation, or biofuel production have been characterized. Many of above-mentioned studies aimed to optimize the cultivation conditions. Factors like the light intensity and quality, the nitrogen source, or different modes of growth (auto-, hetero-, or mixotrophic) have been studied in detail. The scaling-up of the biomass production using photobioreactors, either closed or open, was also investigated to increase the production of useful compounds. The richness of nutrients contained in the genus Arthrospira can be used for promising applications in the biomedical domain. Ingredients such as the calcium spirulan, immulina, C-phycocyanin, and γ-linolenic acid (GLA) show a strong biological activity. Recently, its use in the fight against cancer cells was documented in many publications. The health-promoting action of “Spirulina” has been demonstrated in the case of cardiovascular diseases and age-related conditions. Some compounds also have potent immunomodulatory properties, promoting the growth of beneficial gut microflora, acting as antimicrobial and antiviral. Products derived from Arthrospira were shown to successfully replace biomaterial scaffolds in regenerative medicine. Supplementation with the cyanobacterium also improves the health of livestock and quality of the products of animal origin. They were also used in cosmetic preparations.

show abstract

Spirulina phycocyanin induces differential protein expression and apoptosis in SKOV-3 cells

Cited by 34 publications

References 32 publications

Induction of interleukin-6 trigger an apoptosis through IL-17 and stat3 pathway that alleviated by phycocyanin treatment

Induction of interleukin-6 trigger an apoptosis through IL-17 and stat3 pathway that alleviated by phycocyanin treatment

Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 CellsIn VitroandIn Vivo

Edible Cyanobacterial Genus Arthrospira: Actual State of the Art in Cultivation Methods, Genetics, and Application in Medicine

Contact Info

Product

Resources

About