Spirofungins A (I) and B (II): A Reassignment of Kiyota′s Spiroketals.

Oliveira, Luciana G. de; Dias, Luiz C.; Sakauchi, Hiroyuki; Kiyota, Hiromasa

doi:10.1002/chin.200630214

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Effect Of Denaturing Agents On Dpt Oxidation1

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2017

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“…As denaturing agent, urea affects the secondary and tertiary structure without affecting the primary structure, the surfactant SDS dissolves hydrophobic proteins, and the reducing agent DTT disrupts disulphide bonds, which are essential for qualitative and quantitative analysis in proteomic mechanism investigation [31]. Urea and SDS are not electroactive, unlike DTT, which is electroactive [32].…”

Section: Effect Of Denaturing Agents On Dpt Oxidationmentioning

confidence: 99%

Cyclic Lipopeptide Antibiotic Daptomycin Electrochemical Oxidation at a Glassy Carbon Electrode

Machini

Oliveira-Brett

2017

Electroanalysis

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Daptomycin was the first approved drug from a new class of antimicrobials, the cyclic lipopeptides, and presents a broad spectrum of activity against a wide range of gram‐positive bacteria. The daptomycin redox behaviour, by cyclic, differential pulse and square wave voltammetry, in a wide pH range, at a glassy carbon electrode, was investigated. The daptomycin oxidation was a two‐step irreversible diffusion‐controlled process and the diffusion coefficient DDPT = 2.32 × 10−5 cm2 s−1, was calculated. A detection limit LOD = 0.32 μM, was obtained. For the first time daptomycin, in fetal bovine serum biological fluid, using DP voltammetry, was determined.

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Section: Effect Of Denaturing Agents On Dpt Oxidationmentioning

confidence: 99%