Spiribacter roseus sp. nov., a moderately halophilic species of the genus Spiribacter from salterns

León, María José; Vera-Gargallo, Blanca; Sánchez‐Porro, Cristina; Ventosa, António

doi:10.1099/ijsem.0.001338

Cited by 19 publications

(14 citation statements)

References 26 publications

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“…In summary, our data suggest that ectoine biosynthesis and glycine betaine/arsenobetaine uptake are key features for the physiological adjustment process of S. salinus M19-40 to sustained but moderately high saline habitats. Nevertheless, the growth profile of S. salinus M19-40 suggests that it is optimally adapted to a rather narrow range of salinities ( Figure 1 ), an observation that is consistent with cultivation experiments and metagenomic data of the habitats from which different members of this genus were isolated ( León et al, 2013 , 2014 , 2015 , 2016 , 2017 ; Lopez-Perez et al, 2013 ). However, its ability to scavenge compatible solutes, in particular glycine betaine and arsenobetaine, from environmental sources will allow S. salinus M19-40 to proliferate under otherwise growth-restricting salinities.…”

Section: Discussionsupporting

confidence: 80%

“…Members of the genus Spiribacter are abundant but elusive inhabitants of hypersaline ecosystems. The currently known species of the genus Spiribacter, Spiribacter salinus M19-40 ( León et al, 2014 ), Spiribacter curvatus UAH-SP71 ( León et al, 2015 ), Spiribacter roseus SSL50 ( León et al, 2016 ), and Spiribacter aquaticus SP30 ( León et al, 2017 ), have all been described as moderate halophiles. Depending on the particular species of these Gram-negative bacteria, members of the genus Spiribacter can grow in laboratory media with salinities ranging between 7.5 and 25% (w/v) and are worldwide distributed, as inferred from metagenomic studies of saltern ponds from Santa Pola (on the East coast of Spain), Isla Cristina (Southwest of Spain), and San Diego (CA, United States) ( Lopez-Perez et al, 2013 ; Fernandez et al, 2014b ; León et al, 2014 , 2015 ; Ventosa et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…Members of the genus Spiribacter belong to the family Ectothiorhodospiraceae within the Gammaproteobacteria . They represent the first ecologically defined moderate halophiles and are environmentally abundant inhabitants in various hypersaline ecosystems ( Lopez-Perez et al, 2013 ; Fernandez et al, 2014b ; León et al, 2014 , 2015 , 2016 , 2017 ). Therefore, the type species of this genus, S. salinus M19-40 ( León et al, 2014 ), can be considered as an interesting model organism for studies aiming at understanding how moderate halophiles cope with increased salinity, a trait critical for growth in and lasting colonization of the ecosystem they populate.…”

Section: Introductionmentioning

confidence: 99%

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Compatible Solute Synthesis and Import by the Moderate Halophile Spiribacter salinus: Physiology and Genomics

et al. 2018

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Members of the genus Spiribacter are found worldwide and are abundant in ecosystems possessing intermediate salinities between seawater and saturated salt concentrations. Spiribacter salinus M19-40 is the type species of this genus and its first cultivated representative. In the habitats of S. salinus M19-40, high salinity is a key determinant for growth and we therefore focused on the cellular adjustment strategy to this persistent environmental challenge. We coupled these experimental studies to the in silico mining of the genome sequence of this moderate halophile with respect to systems allowing this bacterium to control its potassium and sodium pools, and its ability to import and synthesize compatible solutes. S. salinus M19-40 produces enhanced levels of the compatible solute ectoine, both under optimal and growth-challenging salt concentrations, but the genes encoding the corresponding biosynthetic enzymes are not organized in a canonical ectABC operon. Instead, they are scrambled (ectAC; ectB) and are physically separated from each other on the S. salinus M19-40 genome. Genomes of many phylogenetically related bacteria also exhibit a non-canonical organization of the ect genes. S. salinus M19-40 also synthesizes trehalose, but this compatible solute seems to make only a minor contribution to the cytoplasmic solute pool under osmotic stress conditions. However, its cellular levels increase substantially in stationary phase cells grown under optimal salt concentrations. In silico genome mining revealed that S. salinus M19-40 possesses different types of uptake systems for compatible solutes. Among the set of compatible solutes tested in an osmostress protection growth assay, glycine betaine and arsenobetaine were the most effective. Transport studies with radiolabeled glycine betaine showed that S. salinus M19-40 increases the pool size of this osmolyte in a fashion that is sensitively tied to the prevalent salinity of the growth medium. It was amassed in salt-stressed cells in unmodified form and suppressed the synthesis of ectoine. In conclusion, the data presented here allow us to derive a genome-scale picture of the cellular adjustment strategy of a species that represents an environmentally abundant group of ecophysiologically important halophilic microorganisms.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Compatible Solute Synthesis and Import by the Moderate Halophile Spiribacter salinus: Physiology and Genomics

et al. 2018

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“…DNA–DNA hybridization studies were carried out following a competition procedure in a nitrocelulose membrane (Johnson, 1994) as described elsewhere (Arahal et al, 2001a,b; León et al, 2016). The range of hybridization temperature used was between 51.8 and 55.0°C, which is within the limit of validity for the filter method used in this study (De Ley and Tijtgat, 1970).…”

Section: Methodsmentioning

confidence: 99%

Assessment of MultiLocus Sequence Analysis As a Valuable Tool for the Classification of the Genus Salinivibrio

et al. 2017

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The genus Salinivibrio includes obligatory halophilic bacteria and is commonly isolated from hypersaline habitats and salted food products. They grow optimally between 7.5 and 10% salts and are facultative anaerobes. Currently, this genus comprises four species, one of them, S. costicola, with three subspecies. In this study we isolated and characterized an additional 70 strains from solar salterns located in different locations. Comparative 16S rRNA gene sequence analysis identified these strains as belonging to the genus Salinivibrio but could not differentiate strains into species-like groups. To achieve finer phylogenetic resolution, we carried out a MultiLocus Sequence Analysis (MLSA) of the new isolates and the type strains of the species of Salinivibrio based on the individual as well as concatenated sequences of four housekeeping genes: gyrB, recA, rpoA, and rpoD. The strains formed four clearly differentiated species-like clusters called phylogroups. All of the known type and subspecies strains were associated with one of these clusters except S. sharmensis. One phylogroup had no previously described species coupled to it. Further DNA–DNA hybridization (DDH) experiments with selected representative strains from these phylogroups permitted us to validate the MLSA study, correlating the species level defined by the DDH (70%) with a 97% cut-off for the concatenated MLSA gene sequences. Based on these criteria, the novel strains forming phylogroup 1 could constitute a new species while strains constructing the other three phylogroups are members of previously recognized Salinivibrio species. S. costicola subsp. vallismortis co-occurs with S. proteolyticus in phylogroup 4, and separately from other S. costicola strains, indicating its need for reclassification. On the other hand, genome fingerprinting analysis showed that the environmental strains do not form clonal populations and did not cluster according to their site of cultivation. In future studies regarding the classification and identification of new Salinivibrio strains we recommend the following strategy: (i) initial partial sequencing of the 16S rRNA gene for genus-level identification; (ii) sequencing and concatenation of the four before mentioned housekeeping genes for species-level discrimination; (iii) DDH experiments, only required when the concatenated MLSA similarity values among a new isolate and other Salinivibrio strains are above the 97% cut-off.

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“…Moreover this analysis also brought to light that a large number of haloarchaeal members have not been isolated until date [7,8]. On the other hand, several culture-dependent studies performed in these salterns have permitted the isolation and characterization of new groups of halophilic bacteria, such as members of the genera Spiribacter [9,10], Idiomarina [11], Marinobacter [12], or Salinivibrio [13], as well as of the archaeal genera Halonotius [14,15] and Halorientalis [16]. In 2016, as part of a new study of the halophilic archaeal diversity of Isla Cristina marine saltern, we isolated a halophilic archaeal strain, designated F16-60 T , closely related to members of the order Halobacteriales.…”

Section: Introductionmentioning

confidence: 98%

Haloglomus irregulare gen. nov., sp. nov., a New Halophilic Archaeon Isolated from a Marine Saltern

2020

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A halophilic archaeal strain, designated F16-60T, was isolated from Isla Cristina marine saltern in Huelva, Spain. Cells were pleomorphic, irregular, non-motile, and Gram-stain-negative. It produced red-pigmented colonies on agar plates. Strain F16-60T was extremely halophilic (optimum at 30% (w/v) NaCl) and neutrophilic (optimum pH 7.5). Phylogenetic tree reconstructions based on 16S rRNA and rpoB´ gene sequences revealed that strain F16-60T was distinct from species of the related genera Natronomonas, Halomarina, and Halomicrobium, of the order Halobacteriales. The polar lipids are phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS), and one glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The DNA G+C content is 68.0 mol%. The taxonomic study, based on a combination of phylogenetic, genomic, chemotaxonomic, and phenotypic analyses, suggest that strain F16-60T (= CECT 9635T = JCM 33318T), represents a novel species of a new genus within the family Haloarculaceae and the order Halobacteriales, for which the name Haloglomus irregulare gen. nov., sp. nov. is proposed. Metagenomic fragment recruitment analysis revealed the worldwide distribution of members of this genus and suggested the existence of other closely related species to be isolated.

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Spiribacter roseus sp. nov., a moderately halophilic species of the genus Spiribacter from salterns

Cited by 19 publications

References 26 publications

Compatible Solute Synthesis and Import by the Moderate Halophile Spiribacter salinus: Physiology and Genomics

Compatible Solute Synthesis and Import by the Moderate Halophile Spiribacter salinus: Physiology and Genomics

Assessment of MultiLocus Sequence Analysis As a Valuable Tool for the Classification of the Genus Salinivibrio

Haloglomus irregulare gen. nov., sp. nov., a New Halophilic Archaeon Isolated from a Marine Saltern

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