Spire localization<i>via</i>zinc finger—containing domain is crucial for the asymmetric division of mouse oocyte

Jo, Yu-Jin; Lee, Inwon; Jung, Seung-Min; Kwon, Jeongwoo; Kim, Nam‐Hyung; Namgoong, Suk

doi:10.1096/fj.201801905r

Cited by 11 publications

(6 citation statements)

References 60 publications

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“…The knockdown of AIP1 induced an increase in the amount of large actin‐positive patches around the spindle region. Similar phenotypes were observed in the depletion of actin nucleators, such as Formin2, 38 Spire 46 or treatment with CytoD, a widely used inhibitor of actin dynamics 38,40 . Moreover, in AIP1 knockdown cells, F‐actin accumulation colocalized with enriched total‐Cofilin in the shortened protrusions.…”

Section: Discussionsupporting

confidence: 61%

AIP1 and Cofilin control the actin dynamics to modulate the asymmetric division and cytokinesis in mouse oocytes

et al. 2020

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Actin-interacting protein 1 (AIP1), also known as WD repeat-containing protein 1 (WDR1), is ubiquitous in eukaryotic organisms, and it plays critical roles in the dynamic reorganization of the actin cytoskeleton. However, the biological function and mechanism of AIP1 in mammalian oocyte maturation is still largely unclear. In this study, we demonstrated that AIP1 boosts ADF/Cofilin activity in mouse oocytes. AIP1 is primarily distributed around the spindle region during oocyte maturation, and its depletion impairs meiotic spindle migration and asymmetric division. The knockdown of AIP1 resulted in the gathering of a large number of actin-positive patches around the spindle region. This effect was reduced by human AIP1 (hAIP1) or Cofilin (S3A) expression. AIP1 knockdown also reduced the phosphorylation of Cofilin near the spindle, indicating that AIP1 interacts with ADF/Cofilin-decorated actin filaments and enhances filament disassembly. Moreover, the deletion of AIP1 disrupts Cofilin localization in metaphase I (MI) and induces cytokinesis defects in metaphase II (MII). Taken together, our results provide evidence that AIP1 promotes actin dynamics and cytokinesis via Cofilin in the gametes of female mice.

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Section: Discussionsupporting

confidence: 61%

AIP1 and Cofilin control the actin dynamics to modulate the asymmetric division and cytokinesis in mouse oocytes

et al. 2020

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“…We found some reproduction-related genes in the GC group. For example, lhx9 encodes a key transcription factor in gonadal development ( Balasubramanian et al, 2014 ), and spir localization is critical for mouse oocyte asymmetric division ( Jo et al, 2019 ; Figure 1C , Figure 1—figure supplement 2E ). Hence, we annotated this group of cells as GCs.…”

Section: Resultsmentioning

confidence: 99%

Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes

Peng

Chen

Huang

et al. 2022

eLife

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Despite the importance of innate immunity in invertebrates, the diversity and function of innate immune cells in invertebrates are largely unknown. Using single-cell RNA-seq, we identified prohemocytes, monocytic hemocytes, and granulocytes as the three major cell-types in the white shrimp hemolymph. Our results identified a novel macrophage-like subset called monocytic hemocytes 2 (MH2) defined by the expression of certain marker genes, including Nlrp3 and Casp1. This subtype of shrimp hemocytes is phagocytic and expresses markers that indicate some conservation with mammalian macrophages. Combined, our work resolves the heterogenicity of hemocytes in a very economically important aquatic species and identifies a novel innate immune cell subset that is likely a critical player in the immune responses of shrimp to threatening infectious diseases affecting this industry.

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“…For example, lhx9 has been shown as a key transcription factor in gonadal development(Balasubramanian, Bui, Xie, Deng, & Gan, 2014). spir localization is critical for mouse oocyte asymmetric division(Jo et al, 2019)( Figure 1C; figure supplement 2E ). Hence, we annotated this group of cells as germ-like cells.…”

Section: Resultsmentioning

confidence: 99%

Myeloid cell evolution uncovered by shrimp immune cell analysis at single-cell resolution

Peng

Chen

Huang

et al. 2022

Preprint

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How myeloid cells evolved from invertebrate to vertebrate is still a mystery. Here we collected circulating hemocytes from a marine invertebrate-Penaeus vannamei via gradient centrifugation and identified prohemocyte, monocytic hemocyte and granulocyte as three major types of cells in shrimp hemolymph by single-cell mRNA sequencing. Additional pseudotime trajectory analysis revealed that shrimp monocytic hemocytes and granulocytes were differentiated from a common progenitor which was similar with that of human myeloid cells. More interestingly, we identified that MH2, a terminal differentiated monocytic hemocyte, was a macrophage-like phagocytic cell which could engulf fluorescein labelled Vibrio parahaemolyticus and shared nine marker genes including inflammasome components Nlrp3 and Casp1 with human macrophage. After that, we compared our classification with traditional shrimp hemocytes classification and found that hyalinocyte included both prohemocyte and monocytic hemocyte while semi-granulocyte included both monocytic hemocyte and granulocyte. In general, our results redefine shrimp hemocyte classification based on functional marker genes and unveil evolutionary trace of myeloid cells in marine invertebrate.

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Spire localizationviazinc finger—containing domain is crucial for the asymmetric division of mouse oocyte

Cited by 11 publications

References 60 publications

AIP1 and Cofilin control the actin dynamics to modulate the asymmetric division and cytokinesis in mouse oocytes

AIP1 and Cofilin control the actin dynamics to modulate the asymmetric division and cytokinesis in mouse oocytes

Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes

Myeloid cell evolution uncovered by shrimp immune cell analysis at single-cell resolution

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