SPINT1-AS1 Drives Cervical Cancer Progression via Repressing miR-214 Biogenesis

Song, Hongjuan; Liu, Yuan; Liang, Hui; Jin, Xin; Liu, Liping

doi:10.3389/fcell.2021.691140

Cited by 12 publications

(6 citation statements)

References 43 publications

(59 reference statements)

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“…Song et al showed that SPINT1-AS1 promoted cervical cancer cell metastasis in vivo and in vitro by suppressing miR-214 expression. thereby inactivating the Wnt/β-catenin signaling pathway (14). Zhen et al showed that lncRNA SPINT1-AS1 was involved in regulating sensitivity to lapatinib.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of lncRNA SPINT1-AS1 by miR-200c/ZEB1 axis inhibits metastasis of breast cancer cells

Liu

et al. 2022

Preprint

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Background Breast cancer is the most frequent and lethal malignancy among females worldwide. Metastasis crucially affects breast cancer-related deaths. Long noncoding RNAs (lncRNAs) are known to perform a crucial function in the regulation of biological processes in a variety of malignancies. The aim of this study was to evaluate the unidentified function played by the lncRNA SPINT1-AS1 and the relevant mechanism in the modulation of breast cancer-related metastasis. Methods Techniques such as immunohistochemistry (IHC) staining, western blotting, quantitative real-time polymerase chain reaction (qRT-PCR), and immunofluorescence (IF) were applied to evaluate RNA and protein expression profiles. In vitro Transwell and wound healing tests, as well as in vivo lung metastasis experiments, were conducted to validate the cell's ability to invade and migrate. To identify the transcription factors that are involved in lncRNA SPINT1-AS1 interactions, the Chromatin Immunoprecipitation(ChIP) and dual-luciferase reporter assays were carried out. Finally, the role of lncRNA SPINT1-AS1 was evaluated in patients with breast cancer. Results lncRNA SPINT1-AS1 and miR-200c showed low levels of expression in breast cancer cell lines. Furthermore, the overexpression of lncRNA SPINT1-AS1 or miR-200c in the cell lines decreased the tumor cell invasion. Mechanistically, miR-200c induced the overexpression of the lncRNA SPINT1-AS1 via the mechanism of causing zinc finger E-box-binding homeobox members 1 (ZEB1) to bind directly to the promoter DNA of the lncRNA SPINT1-AS1. The miR-200c mediated upregulation of the lncRNA SPINT1-AS1 suppressed breast cancer cell metastasis via the STAT3 signaling pathway. Conclusion lncRNA SPINT1-AS1, which was up-regulated by the miR-200c/ZEB1 axis, performs a crucial function in inhibiting metastasis of breast cancer cells via the Phosphorylation-signal transducer and activator of transcription 3(p-STAT3) pathway and can be a novel prognostic and treatment target for the disease.

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Section: Discussionmentioning

confidence: 99%

Upregulation of lncRNA SPINT1-AS1 by miR-200c/ZEB1 axis inhibits metastasis of breast cancer cells

Liu

et al. 2022

Preprint

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“…For example, in cervical cancer, several lncRNAs, including RP11-480112.5 , ASB16-AS1 , HOTAIR , CASC11 , CALML3-AS1 , and DANCER , are involved in modulating Wnt Signaling pathways [ 35 ]. More recently, several additional lncRNAs, including SNHG6 [ 37 ], EGFR-AS1 [ 38 ], SPINT1-AS1 [ 39 ], HNRNPU-AS1 [ 40 ], and LINC00665 [ 41 ] have been identified that contribute to the proliferation, migration, invasion, and EMT in cervical cancer via the Wnt signaling pathway ( Table 1 , Figure 1 ).…”

Section: Lncrnas-regulated Pathways In Cervical Cancermentioning

confidence: 99%

The Regulatory Functions and the Mechanisms of Long Non-Coding RNAs in Cervical Cancer

Yang

Al-Hendy

2022

Cells

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Cervical cancer is one of the leading causes of death in gynecology cancer worldwide. High-risk human papillomaviruses (HPVs) are the major etiological agents for cervical cancer. Still, other factors also contribute to cervical cancer development because these cancers commonly arise decades after initial exposure to HPV. So far, the molecular mechanisms underlying the pathogenesis of cervical cancer are still quite limited, and a knowledge gap needs to be filled to help develop novel strategies that will ultimately facilitate the development of therapies and improve cervical cancer patient outcomes. Long non-coding RNAs (lncRNAs) have been increasingly shown to be involved in gene regulation, and the relevant role of lncRNAs in cervical cancer has recently been investigated. In this review, we summarize the recent progress of ascertaining the biological functions of lncRNAs in cervical cancer, from the perspective of cervical cancer proliferation, invasion, and metastasis. In addition, we provide the current state of knowledge by discussing the molecular mechanisms underlying the regulation and emerging role of lncRNAs in the pathogenesis of cervical cancer. Comprehensive and deeper insights into lncRNA-mediated alterations and interactions in cellular events will help develop novel strategies to treat patients with cervical cancer.

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“…Moreover, we also observed that several of those miRNAs were common to several types of cancer ( Figure 2 ). Interestingly, of the 15 cancer types that we selected, miR-214 was reported as deregulated in all of them [ 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 ]. As previously stated, this miRNA targets TMPRSS2, which is crucial for SARS-CoV-2 entry into the cells.…”

Section: Sars-cov-2-related Mirnas Deregulation Impact On Cancer Pati...mentioning

confidence: 99%

“…Therefore, a downregulation of this miRNA can lead to an increased expression of TMPRSS2 and an increased susceptibility to SARS-CoV-2 infection. In addition to this, miR-214 is known for is tumorigenic and suppressive roles in cancer, and it is also being considered as a potential biomarker and therapeutic agent [ 73 ], apart from in brain and pancreatic cancer, and melanoma were it as an oncogenic role ( Figure 2 ) [ 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 ]. Moreover, miR-98, another miRNA that targets TMPRSS2, was reported as deregulated in 14 of the 15 cancer types that are displayed in Figure 2 (it was not described in kidney cancer).…”

Section: Sars-cov-2-related Mirnas Deregulation Impact On Cancer Pati...mentioning

confidence: 99%

MicroRNAs as Potential Tools for Predicting Cancer Patients’ Susceptibility to SARS-CoV-2 Infection and Vaccination Response

Dias

Teixeira

et al. 2022

Cells

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Coronavirus disease (COVID-19) is an infectious disease that is caused by a highly contagious and severe acute respiratory syndrome—coronavirus 2 (SARS-CoV-2). This infection started to spread across the world in 2019 and rapidly turned into a global pandemic, causing an urgent necessity for treatment strategies development. The mRNA vaccines against SARS-CoV-2 can trigger an immune response, providing genetic information that allows the production of spike glycoproteins. MiRNAs play a crucial role in diverse key cellular processes, including antiviral defense. Several miRNAs are described as key factors in SARS-CoV-2 human infection through the regulation of ACE2 levels and by the inhibition of SARS-CoV-2 replication and spike expression. Consequently, these molecules have been considered as highly promising biomarkers. In numerous human malignancies, it has been recognized that miRNAs expression is dysregulated. Since miRNAs can target SARS-CoV-2-associated mRNAs, in cancer patients, the deregulation of these molecules can impair the immune response to the vaccines. Therefore, in this review, we propose a miRNA profile of seven SARS-CoV-2-related miRNAs, namely miR-214, miR-98-5p, miR-7-5p, miR-24-3p, miR-145-5p, miR-223-3p and miR-15b-5p, that are deregulated in a high number of cancers and have the potential to be used as prognostic biomarkers to stratify cancer patients.

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SPINT1-AS1 Drives Cervical Cancer Progression via Repressing miR-214 Biogenesis

Cited by 12 publications

References 43 publications

Upregulation of lncRNA SPINT1-AS1 by miR-200c/ZEB1 axis inhibits metastasis of breast cancer cells

Upregulation of lncRNA SPINT1-AS1 by miR-200c/ZEB1 axis inhibits metastasis of breast cancer cells

The Regulatory Functions and the Mechanisms of Long Non-Coding RNAs in Cervical Cancer

MicroRNAs as Potential Tools for Predicting Cancer Patients’ Susceptibility to SARS-CoV-2 Infection and Vaccination Response

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