We experimentally study spin dynamics in a sodium antiferromagnetic spinor condensate with off-resonant microwave pulses. In contrast to a magnetic field, a microwave dressing field enables us to explore rich spin dynamics under the influence of a negative net quadratic Zeeman shift qnet. We find an experimental signature to determine the sign of qnet, and observe harmonic spin population oscillations at every qnet except near each separatrix in phase space where spin oscillation period diverges. In the negative and positive qnet regions, we also observe a remarkably different relationship between each separatrix and the magnetization. Our data confirms an important prediction derived from the mean-field theory: spin-mixing dynamics in spin-1 condensates substantially depends on the sign of the ratio of qnet and the spin-dependent interaction energy. This work may thus be the first to use only one atomic species to reveal mean-field spin dynamics, especially the separatrix, which are predicted to appear differently in spin-1 antiferromagnetic and ferromagnetic spinor condensates. [1,9,11,12], and quantum spin-nematic squeezing [14]. Such systems have been successfully described with a classical two-dimensional phase space [1, 2, 15-17], a rotor model [18], or a quantum model [13,17].In this paper, we experimentally study spin-mixing dynamics in a F =1 sodium spinor condensate starting from a nonequilibrium initial state, as a result of antiferromagnetic spin-dependent interactions and the quadratic Zeeman energy q M induced by an off-resonant microwave pulse. In contrast to a magnetic field, a microwave dressing field enables us to explore rich spin dynamics under the influence of a negative net quadratic Zeeman energy shift q net . A method to characterize the microwave dressing field is also explained. In both negative and positive q net regions, we observe spin population oscillations * yingmei.liu@okstate.edu resulted from coherent collisional interconversion among two |F = 1, m F = 0 atoms, one |F = 1, m F = +1 atom, and one |F = 1, m F = −1 atom. In every spin oscillation studied in this paper, our data shows that the population of the m F = 0 state averaged over time is always larger (or smaller) than its initial value as long as q net < 0 (or q net > 0). This observation provides an experimental signature to determine the sign of q net . We also find a remarkably different relationship between the total magnetization m and a separatrix in phase space where spin oscillation period diverges: the position of the separatrix moves slightly with m in the positive q net region, while the separatrix quickly disappears when m is away from zero in the negative q net region. Our data confirms an important prediction derived by Ref. [17]: the spin-mixing dynamics in F =1 spinor condensates substantially depends on the sign of R = q net /c. This work may thus be the first to use only one atomic species to reveal mean-field spin dynamics, especially the separatrix, which are predicted to appear differently in F =1 an...