SPINK1, macrophages et cellules progénitrices du foie

Lanthier, Nicolas

doi:10.1051/medsci/20163202005

Cited by 1 publication

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“…Finally, we explored SPINK1 mRNA expression on liver sections, this also being identified to be at higher levels in improvers in the baseline study [10, 16]. SPINK1 mRNA by in situ hybridization was present at week 4 in SCT patients in the liver parenchyma adjacent to macrophage recruitment and liver cell proliferation areas (Additional file 2).…”

Section: Resultsmentioning

confidence: 99%

Autologous bone marrow-derived cell transplantation in decompensated alcoholic liver disease: what is the impact on liver histology and gene expression patterns?

et al. 2017

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BackgroundLiver stem cell therapy (SCT) has been suggested as a promising means to improve liver regeneration in advanced liver disease. However, data from trials are heterogeneous, with no systematic histological evaluation. The aim of this study is to specifically analyze the effect of autologous SCT on liver regeneration and on gene expression changes.MethodsIndividuals in the randomized controlled trial of SCT in alcoholic hepatitis with paired liver biopsies were included (n = 58). Immunohistochemistry (Ki67, K7, and CD68), in situ hybridization (SPINK1), and global gene expression analysis were performed on liver biopsies (30 control patients and 28 patients with transarterial administration of bone marrow-derived stem cells) both at baseline and after 4 weeks of follow-up.ResultsNo difference between the two groups could be observed regarding the proliferative hepatocyte number, proliferative K7-positive cells, or total K7-positive cells at the 4-week follow-up liver biopsy. However, patients who received SCT showed a more important liver macrophagic expansion as compared to standard treatment. Transcriptome data revealed changes in genes linked with inflammation (CD68 and SAA), regeneration (SPINK1 and HGF), fibrosis (COL1A1), and stem cells (CD45). No changes in gene pathways involved in liver growth and cell cycle proteins were evident. SPINK1 mRNA was present by in situ hybridization at week 4 in SCT patients in the liver parenchyma areas adjacent to macrophage recruitment and liver cell proliferation.ConclusionsThe analysis of liver tissue after SCT demonstrated an expansion of macrophages concurrent with an upregulated expression of genes involved in inflammatory and regenerative pathways. With the negative results from the clinical trial, the impact of the SCT has to be interpreted as weak, and it is not able to modify the clinical course of this severe liver disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0541-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%