Abstract:<p><strong>Objective</strong>: Quantitative analysis of spindle dynamics in mitosis through fluorescence microscopy requires tracking spindles elongation in noisy image sequences. Deterministic methods, which use typical microtubule detection and tracking methods, perform poorly in the case of the sophisticated background of spindles. <strong>Methods</strong>: In this paper, we present SpindlesTracker, a fully automatic and extensible workflow that can efficiently analyze time-lap… Show more
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