Spindle imaging: a marker for embryo development and implantation

Madaschi, Camila; Bonetti, T.C.; Braga, Daniela Paes de Almeida Ferreira; Pasqualotto, Fábio Firmbach; Iaconelli, Assumpto; Borges, Edson

doi:10.1016/j.fertnstert.2007.05.071

Cited by 66 publications

(60 citation statements)

References 30 publications

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“…So far, it has been reported that the presence of a birefringent meiotic spindle in human oocytes observed using the PolScope is associated with higher fertilization and pregnancy rates [14,[24][25][26]. The location of the meiotic spindle close to the first polar body was correlated with fertilization and cleavage rates [25] and embryo quality [27].…”

Section: Discussionmentioning

confidence: 96%

Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection

Tomari

Honjou²,

Nagata³

et al. 2011

J Assist Reprod Genet

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Purpose To investigate the relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection (ICSI). Methods Zygotes that had cleaved to two-cell stage by 27 h post-ICSI were classified as early cleaving and the remainder as late cleaving. Meiotic spindle parameters previously imaged using the PolScope were compared between the two groups. Results Of 384 embryos, 163 were classed as early cleaving and 221 as late cleaving. The rate of blastocyst formation or pregnancy by Day 2 embryo transfer was significantly higher following early cleavage than after late cleavage (52.4% vs. 24.4% or 32.6% vs. 11.4%). Spindle areas (108.0 vs. 89.8 μm 2 ), lengths (14.7 vs. 13.4 μm) and PolScope retardance were also significantly greater in the early cleaving group. Conclusions Meiotic spindle parameters determine the timing of the first zygotic cleavage and are strong indicators of human embryo developmental potential.

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Section: Discussionmentioning

confidence: 96%

Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection

Tomari

Honjou²,

Nagata³

et al. 2011

J Assist Reprod Genet

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“…The percentage of absent or invisible spindles found by different authors varies from 9 to 22% [19,26,29,32], though some authors found higher percentages [24]. Correlations have been described between the presence and absence of the meiotic spindle and fertilization rates [19,[21][22][23][24]26]. Most authors [19,23,24] report that oocytes without a meiotic spindle have poor reproductive outcomes.…”

Section: Spindle Presence/absencementioning

confidence: 99%

“…Correlations have been described between the presence and absence of the meiotic spindle and fertilization rates [19,[21][22][23][24]26]. Most authors [19,23,24] report that oocytes without a meiotic spindle have poor reproductive outcomes.…”

Section: Spindle Presence/absencementioning

confidence: 99%

“…Numerous groups have attempted to demonstrate the utility of polarized light equipment in daily clinical practice, doing so by evaluating the presence and others features of MS and relating it to further development: fertilization rates [19,[21][22][23][24][25][26][27]; cleavage, embryo quality, blastocyst formation [19, 22, 24-26, 28, 29], and pregnancy and implantation rates [27,28,30]. The results of these studies are contradictory, thus calling into question the usefulness of polarized light microscopy in IVF laboratories.…”

Section: Introductionmentioning

confidence: 99%

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Predictive value of spindle retardance in embryo implantation rate

García-Oro¹,

Rey²,

Rodriguez³

et al. 2017

J Assist Reprod Genet

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Purpose We evaluated the relationship between meiotic spindle characteristics and in vitro fertilization cycle outcome. Methods Five hundred sixty-nine oocytes from 86 in vitro fertilization cycles were analyzed for fertilization and subsequent implantation rates. Oocytes were assessed for maturation status. The oocytes and embryos were cultured in sequential and nonsequential media (G Series, Vitrolife, Sweden) and incubated in 6% CO 2 , 5% O 2 at 37°C.Two hours following oocyte decumulation (38-39 h posthCG/GnRH administration) and prior to microinjection, the structure of the meiotic spindle was assessed using the Oosight Imaging System (CRI, UK). Results Four hundred fifty-six oocytes (80.5%) had a visible meiotic spindle, 82 (14.7%) had no meiotic spindle, and 31 (5.5%) were in telophase I. Oocytes exhibiting a meiotic spindle had a significantly higher fertilization rate and a lower rate of abnormal fertilization. Implantation data were obtained for 195 of the embryos transferred. The implantation rate for embryos derived from oocytes with a meiotic spindle was 32.9%, while in embryos originating from oocytes without a meiotic spindle and oocytes in telophase, this value dropped significantly (8.8 and 0%, respectively). To determine the correlation between retardance values and implantation rate for each oocyte, we established four groups, finding a range of retardance values with significantly higher implantation rates (27.5, 21, 29.3, and 53.8%, respectively). Conclusion Meiotic spindle imaging may be a valuable tool for prediction of oocyte quality, and retardance values of meiotic spindles, together with classical morphological classification, can be useful to select embryos with a higher implantation potential.

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“…As far as oocyte morphological criteria, several have been claimed to correlate with outcome, including polar body morphology [8]; cytoplasm appearance [9], and more recently zona pellucida thickness, appearance and birefringence [10][11][12] and the position or shape of the spindle [13]. Also in this case biochemical markers helping in identifying oocytes completing in vitro maturation would be very interesting in IVF approaches.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Proling Reveals Barcode-Like Toxicogenomic Responses in the Zebrash Embryo

2011

Current Research in Embryology

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Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48-72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection.

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Spindle imaging: a marker for embryo development and implantation

Cited by 66 publications

References 30 publications

Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection

Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection

Predictive value of spindle retardance in embryo implantation rate

Transcriptional Proling Reveals Barcode-Like Toxicogenomic Responses in the Zebrash Embryo

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