Spindle disturbances in mammalian cells II. Induction of viable aneuploid/polyploid cells and multiple chromatid exchanges after treatment of V79 Chinese hamster cells with carbaryl modifying effects of glutathione and S9

Önfelt, Agneta; Klášterská, I.

doi:10.1016/0165-7992(83)90180-x

Cited by 35 publications

(10 citation statements)

References 9 publications

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“…Within these same fibroblasts, Onfelt and Klasterska observed that direct exposure to carbaryl induced sister chromatid exchanges and increased the frequency of chromosomal breaking and fragmentation; however, the effects were reduced with the addition of an S9 mix 11. They also observed that carbaryl, due to its ability to interfere with spindle formation in mammalian cells, increased the frequency of viable aneuploid and polyploid cells 12. However, this effect was greatly reduced by simultaneous addition of rat liver microsomes 12…”

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confidence: 97%

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Carbaryl exposure and incident cancer in the Agricultural Health Study

Mahajan

Blair

Coble

et al. 2007

Intl Journal of Cancer

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Carbaryl is a carbamate insecticide with a broad spectrum of uses in agricultural, commercial and household settings. It has previously been linked with non-Hodgkin lymphoma (NHL) but studies of cancer risk in humans are limited. We examined occupational carbaryl use and risk of all cancers in the Agricultural Health Study, a prospective study of a cohort of pesticide applicators in North Carolina and Iowa. This analysis included 21,416 subjects (1,291 cases) enrolled from 1993-1997 and followed for cancer incidence through 2003. Pesticide exposure and other data were collected using self-administered questionnaires. Poisson regression was used to calculate rate ratios (RRs) and 95% confidence intervals (CIs) while controlling for potential confounders. Carbaryl was not associated with cancer risk overall. Relative to subjects who never used carbaryl, melanoma risk was elevated with >175 lifetime exposure-days (RR 5 4.11; 95%CI, 1.33-12.75; p-trend 5 0.07), >10 years of use (RR 5 3.19; 95%CI, 1.28-7.92; p-trend 5 0.04), or 10 days of use per year (RR 5 5.50; 95%CI, 2.19-13.84; p-trend < 0.001). Risk remained after adjusting for sunlight exposure. Although not significant, there appeared to be a trend of decreasing prostate cancer risk with increasing level of exposure. A small increase in NHL risk was observed using some, but not all, exposure measures. No associations were observed with other examined cancer sites. Because the observed results were not hypothesized a priori and because of limited study of their biological plausibility, they should be interpreted with caution. ' 2007 Wiley-Liss, Inc.

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confidence: 97%

“…They also observed that carbaryl, due to its ability to interfere with spindle formation in mammalian cells, increased the frequency of viable aneuploid and polyploid cells 12. However, this effect was greatly reduced by simultaneous addition of rat liver microsomes 12…”

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confidence: 99%

Carbaryl exposure and incident cancer in the Agricultural Health Study

Mahajan

Blair

Coble

et al. 2007

Intl Journal of Cancer

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“…In previous reports, they were found to be nonmutagenic to bacteria (1,2) and mammalian cells (3)(4)(5)(6). Positive results, however, were shown in the induction of sister chromatid exchange (SCE*) (4,7,8), chromosome aberration (6,(8)(9)(10) and aneuploidy (10,11). Propoxur does not induce DNA strand breaks in mammalian cells (3), but is weakly clastogenic (5) and induces SCE and micronuclei in human lymphocytes in vitro (8).…”

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confidence: 99%

Genotoxicity of propoxur and its N-nitroso derivative in mammalian cells

Wang

Chiou²,

Hu³

1998

Carcinogenesis

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N-Nitroso propoxur (NP) can be synthesized from a widely used N-methylcarbamate insecticide, propoxur, in vitro in the laboratory. Because of the extensive use of aerosol propoxur, the adverse effect on cells of respiratory origin is worth elucidating. In this report, two mammalian cell cultures from respiratory tissues [a hamster lung fibroblast, V79, and a primary rat tracheal epithelial cell (RTE)], were used to investigate the genotoxicity of propoxur and NP. NP was more cytotoxic than propoxur, with LC 50 s (20 and six times smaller, respectively in V79 and RTE cells. NP significantly induced sister chromatid exchange (ജ0.01 µg/ml), chromosome aberration (ജ2.5 µg/ml) and hprt gene mutation (ജ0.5 µg/ml) in V79 cells, and cell transformation (ജ0.2 µg/ml) in RTE cells. Results of chromosome aberration and hprt gene mutation indicated that the major pre-mutagenic lesion induced by NP must be the O 6 -methylguanine adduct, which frequently mispairs with thymine and thus gives rise to a GC→AT transition. Propoxur was not mutagenic to either type of cells. However, it inhibited gap-junctional intercellular communication in V79 cells, which indicates that propoxur could act through some epigenetic mechanisms, such as tumor promotion or cell proliferation, in the multiple process of chemical carcinogenesis.

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“…Several other pesticides have also been reported to induce such abnormalities by af fecting mitotic spindles (Grant 1978, Badr 1983, Panda and Sahu 1985, Chauhan et al 1986). Mitotic abnormalities are generally considered insignificant from the mutation point of view unless polyploid or aneuploid cells are induced (Onfelt and Klasterska 1983). Isoproturon frequently induced micronucleated and binucleated cells which may lead to aneuploid and polyploid cells in subsequent mitotic divisions.…”

Section: Discussionmentioning

confidence: 99%