Spinal metastasis of medulloblastoma in adults: A case report

Živković, Nenad; Berisavac, Iva; Marković, Miroslav; Milenković, Sanja

doi:10.2298/sarh1412713z

Cited by 1 publication

(1 citation statement)

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“…Particular complexities arise in MB, because chemoattractant fields that rouse cells away from primary tumors can be secreted by healthy or transformed distant and neighboring cells (1, 3, 9, 34, 58, 76) . Further, clinical studies have illustrated that the spread of MB cells occurs predominantly to the spinal column via a combination of two-dimensional (2D) and three-dimensional (3D) movements (6, 51, 67, 77, 83) . Such spreading results from tumor cells that drop into the cerebrospinal fluid and tend to seed in parts of the spine (53, 74, 77, 79, 84) .…”

Section: Introductionmentioning

confidence: 99%

EGF as a New Therapeutic Target for Medulloblastoma Metastasis

et al. 2015

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Medulloblastoma (MB) is a malignant pediatric brain tumor known for its aggressive metastatic potential. Despite the well-documented migration of MB cells to other parts of the brain and spinal column, MB chemotaxis is poorly understood. Herein, we examined the in vitro migratory and cellular responses of MB-derived cells to external signaling of Epidermal Growth Factor (EGF), hepatocyte growth factor (HGF), platelet-derived growth factor (PDGF-BB), and the stromal cell-derived factors 1-alpha (SDF-1). Experiments utilized transwell assays and immunocytochemistry to identify receptor activation in MB migration, and used a microfluidic platform to examine directionality, trajectory, and gradient-dependence of motile cells. Data illustrates that MB-derived cells respond strongly to EGF in a dosage and gradient-dependent manner with increased EGF-R activation, and show that high EGF gradient fields cause an increased number of cells to migrate longer directed distances. Our results provide evidence that EGF and its receptor play an important role than previously documented in MB chemotactic migration than previously documented and should be considered for developing migration-target therapies against MB metastasis.

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