Spinal Cord Neuronal Network Formation in a 3D Printed Reinforced Matrix—A Model System to Study Disease Mechanisms (Adv. Healthcare Mater. 19/2021)

Fischhaber, Natalie; Faber, Jessica; Bakırcı, Ezgi; Dalton, Paul D.; Budday, Silvia; Villmann, Carmen; Schaefer, Natascha

doi:10.1002/adhm.202170090

Cited by 2 publications

(2 citation statements)

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“…The study demonstrates that the 3D tissue model of spinal cord neuronal networks in the ultrasoft matrix reinforced by MEW fibers exhibit mature inhibitory synapse formation, mechanical properties resembling native spinal cord tissue, and intensive neuronal activity after a certain period of culture. [18] Włodarczyk-Biegun et al utilized MEW to create porous scaffolds that mimic the matrix structure of the in vitro model of human trabecular meshwork (HTM). Primary HTM cells attached to the scaffolds, proliferated, and formed a confluent layer, showing high cell viability and morphology similar to native tissue.…”

Section: Bone Tissuementioning

confidence: 99%

“…These kinds of scaffolds help to guide the cell growth in the direction of gradient immobilized over the scaffold. This will lead to the fabrication of in vitro model of different tissue as previously discussed in the literature for bone, [8][9][10][11][12] muscle, [14,15] nerve, [18,19] cartilage, [20,21] vascular, [13] acetabular labrum, [16] and meniscus. [17] This technique opens up a new avenue to utilize the architectural design variable in the favor of biological outcome by controlling the amount of attached EGF into the scaffolds and can be used for fabrication of in vitro model of tissue and tested in vivo in future.…”

Section: Postprocess Approachmentioning

confidence: 99%

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Gradient Biomolecular Immobilization of 3D Structured Poly‐ɛ‐caprolactone Biomaterials toward Functional Engineered Tissue

Zaeri,

Zgeib,

Zhang

et al. 2023

Macro Materials & Eng

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Additive manufacturing (AM) enables the tailored production of precision fibrous scaffolds toward various engineered tissue models. Moreover, by functionalizing scaffolds in either a uniform or gradient pattern of biomolecules, different target tissues can be fabricated in vitro to capture key characteristics of in vivo cellular microenvironments. However, current engineered tissue models lack the appropriate cellular cues that are needed to deterministically direct cell behavior. Specifically, tunable and reproducible scaffold‐guided stimuli are identified herein as the missing link between biomaterial structure and cellular behavior. Therefore, the bottleneck of precision control is addressed here over the immobilization of patterned biomolecular stimuli with either uniform or gradient distribution over the AM‐enabled 3D biomaterial model as a function of different growth factors exposure variables, protocols, and various scaffold architectural design parameters. The produced study outcomes herein will improve the directing and guiding of biological cell attachment and growth direction in the context of scaffold‐guided stimuli techniques. Therefore, unprecedented control is presented here over 3D structured biomaterial gradient functionalization and immobilization of biomolecules toward biomimetic tissue architectures.

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Section: Bone Tissuementioning

confidence: 99%

Section: Postprocess Approachmentioning

confidence: 99%

Gradient Biomolecular Immobilization of 3D Structured Poly‐ɛ‐caprolactone Biomaterials toward Functional Engineered Tissue

Zaeri,

Zgeib,

Zhang

et al. 2023

Macro Materials & Eng

View full text Add to dashboard Cite

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Application of three-dimensional printing technology to the customized design of spinal implants

Yang,

Zhang,

Liu

et al. 2024

Applied Mathematics and Nonlinear Sciences

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In recent years, the field of 3D printing technology has experienced rapid advancements, notably expanding its application within the medical sector. This study focuses on the custom design of 3D-printed spinal implants, specifically examining porous interbody fusion products. It integrates considerations of mechanical strength and bone ingrowth to establish a finite element model of porous interbody fusion, subsequently conducting topology optimization to design three distinct types of spinal interbody fusion implants. Analytical investigations were carried out on the stress and displacement responses of these three implant types under compressive loading. Furthermore, a detailed stress analysis was conducted on implants varying in porosity, length, and screw angle of the bone graft to assess the performance characteristics of the porous interbody fusion devices. Results indicated that the Type C implant exhibited superior performance, demonstrating a stress reduction to 89.21 MPa and a displacement change of 0.006 mm, optimally at a 60% porosity level. Adjustments in the lengths and screw clamp angles of the splint ensured that the maximal stress experienced by each vertebra remained below the yield limits of both cortical and cancellous bone, thus preventing vertebral damage. This paper presents a comparative analysis of three types of porous interbody fusion devices, providing substantial data support and a theoretical framework that can inform the future development of fusion products.

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Spinal Cord Neuronal Network Formation in a 3D Printed Reinforced Matrix—A Model System to Study Disease Mechanisms (Adv. Healthcare Mater. 19/2021)

Cited by 2 publications

References 0 publications

Gradient Biomolecular Immobilization of 3D Structured Poly‐ɛ‐caprolactone Biomaterials toward Functional Engineered Tissue

Gradient Biomolecular Immobilization of 3D Structured Poly‐ɛ‐caprolactone Biomaterials toward Functional Engineered Tissue

Application of three-dimensional printing technology to the customized design of spinal implants

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