Spinal cord injury repair using mesenchymal stem cells derived from bone marrow in mice: A stereological study

Khodabandeh, Zahra; Mehrabani, Davood; Dehghani, Farzaneh; Gashmardi, Nooshin; Erfanizadeh, Mahboobeh; Zare, Shahrokh; Bozorg-Ghalati, Farzaneh

doi:10.1016/j.acthis.2021.151720

Cited by 11 publications

(4 citation statements)

References 31 publications

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“…In TSCI, BM-MSC treatment aims for optimal neuronal regeneration by limitation of the secondary injury and modulation of the local microenvironment in order to optimise healing cascades and axonal regeneration as well as by targeting differentiation of cells to restore spinal cord function [ 17 , 19 , 266 ]. MSC transplantation after TSCI has shown promising results in some preclinical studies [ 258 , 267 , 268 , 269 ]. These results were translatable to the chronic phase of the injury in mice [ 259 , 270 ].…”

Section: Tbi Tsci and Mscsmentioning

confidence: 99%

Future Perspectives in Spinal Cord Repair: Brain as Saviour? TSCI with Concurrent TBI: Pathophysiological Interaction and Impact on MSC Treatment

Köhli

Otto

Jahn

et al. 2021

Cells

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Traumatic spinal cord injury (TSCI), commonly caused by high energy trauma in young active patients, is frequently accompanied by traumatic brain injury (TBI). Although combined trauma results in inferior clinical outcomes and a higher mortality rate, the understanding of the pathophysiological interaction of co-occurring TSCI and TBI remains limited. This review provides a detailed overview of the local and systemic alterations due to TSCI and TBI, which severely affect the autonomic and sensory nervous system, immune response, the blood–brain and spinal cord barrier, local perfusion, endocrine homeostasis, posttraumatic metabolism, and circadian rhythm. Because currently developed mesenchymal stem cell (MSC)-based therapeutic strategies for TSCI provide only mild benefit, this review raises awareness of the impact of TSCI–TBI interaction on TSCI pathophysiology and MSC treatment. Therefore, we propose that unravelling the underlying pathophysiology of TSCI with concomitant TBI will reveal promising pharmacological targets and therapeutic strategies for regenerative therapies, further improving MSC therapy.

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Section: Tbi Tsci and Mscsmentioning

confidence: 99%

Future Perspectives in Spinal Cord Repair: Brain as Saviour? TSCI with Concurrent TBI: Pathophysiological Interaction and Impact on MSC Treatment

Köhli

Otto

Jahn

et al. 2021

Cells

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“…These cells possess strong bioactivity for modulating inflammation, enhancing angiogenesis, reducing tissue fibrosis, as well as immunosuppressive effects, multipotential differentiation abilities, self-renewal potential, and migratory properties, making them a candidate for clinical applications [ 2 , 3 ]. MSCs have successfully been used in regenerative medicine and isolated from various tissues including bone marrow [ 4 ], adipose [ 5 ], amniotic fluid [ 6 ], Wharton’s jelly [ 7 ], and dental pulp [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI

Jalli

Mehrabani

Zare

et al. 2023

JCM

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To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the internalization of these nanoparticles. Cell proliferation, viability, differentiation, and apoptosis were investigated. Prussian blue staining and MRI were used to trace transfected labeled cells. DPSCs were a morphologically spindle shape, adherent to culture plates, and positive for adipogenic and osteogenic inductions. They expressed CD73 and CD90 markers and lacked CD34 and CD45. Iron oxide labeling and transfection with lipofectamine in DPSCs had no toxic impact on viability, proliferation, and differentiation, and did not induce any apoptosis. In vitro and in vivo internalization of iron oxide nanoparticles within DPSCs were confirmed by Prussian blue staining and MRI tracking. Prussian blue staining and MRI tracking in the absence of any toxic effects on cell viability, proliferation, differentiation, and apoptosis were safe and accurate to track DPSCs labeled with iron oxide and transfected with lipofectamine. MRI can be a useful imaging modality when treatment outcome is targeted.

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“…The transplantation of stem cells provides a potential method to replace injured cells at lesion sites for the repair of SCI, and bone marrow mesenchymal stem cells (BMSCs) are one of the most studied cell types (14)(15)(16). BMSCs facilitate the healing of ischemic tissue-related diseases through proangiogenic secretory proteins (17).…”

Section: Introductionmentioning

confidence: 99%

Quantitative iTRAQ proteomics reveal the proteome profiles of bone marrow mesenchymal stem cells after cocultures with Schwann cells in vitro

Ding¹,

Li²,

Sun³

et al. 2022

Ann Transl Med

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Background: Bone marrow mesenchymal stem cells (BMSCs) combined with Schwann cells (SCs) represent a better therapeutic cell transplantation strategy for treating spinal cord injury (SCI) than transplantation with BMSCs or SCs alone. In previous studies, we demonstrated that BMSCs are able to differentiate in neuron-like cells when cocultured with SCs. The detailed mechanism underlying SCI repair that occurs during the combined transplantation of BMSCs and SCs has not yet been studied. In this study, we adopted an isobaric tag for relative and absolute quantitation (iTRAQ)-based protein identification/ quantification approach to examine the effects of the SC and BMSC coculture process on the BMSCs and then obtained and analyzed the differentially expressed proteins (DEPs) and their possible related pathways.Methods: This study included three groups based on the number of coculture days (i.e., 0, 3, and 7 days).Changes in BMSC protein expression levels were measured using the iTRAQ technique. A bioinformatics analysis of all the data was performed.Results: In total, 6,760 types of proteins were detected, corresponding to 5,181 data points with quantitative information. Of these, a total of 243 DEPs were identified, of which 169 proteins were upregulated and 74 proteins were downregulated. These DEPs were identified by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Intercellular adhesion molecule-1 (ICAM-1), integrin, and dioxygenase may play crucial roles in the repair of SCI. The data analysis indicates that the relevant biological processes may be regulated by lysosome function, cell adhesion molecules (CAMs), leukocyte transendothelial migration, and the phosphatidylinositol-3-kinase (PI3K) and peroxisome proliferator-activated receptor (PPAR) signaling pathways. Conclusions:The data provided in this study indicate that several molecular mechanisms and signaling pathways are involved in the BMSC and SC coculture process. This information may be useful for the further identification of specific targets and related mechanisms and guide new directions for SCI treatment.

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Spinal cord injury repair using mesenchymal stem cells derived from bone marrow in mice: A stereological study

Cited by 11 publications

References 31 publications

Future Perspectives in Spinal Cord Repair: Brain as Saviour? TSCI with Concurrent TBI: Pathophysiological Interaction and Impact on MSC Treatment

Future Perspectives in Spinal Cord Repair: Brain as Saviour? TSCI with Concurrent TBI: Pathophysiological Interaction and Impact on MSC Treatment

Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI

Quantitative iTRAQ proteomics reveal the proteome profiles of bone marrow mesenchymal stem cells after cocultures with Schwann cells in vitro

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