Spin trapping of free radicals generated by suspensions of microcarrier bead-adherent endothelial cell monolayers. B.E.

In Vitro Systems for Studying Respiratory System Toxicology

“…Cell harvesting and isolation techniques permit scientists to evaluate many of the approximately 40 different respiratory tract cell types in relatively homogenous primary culture (Britigan et al 1992;Dobbs 1990;Leikauf and Driscoll 1993;Murphy et al 1995;Netteshein et al 1990;Patel et al 1990;Renier et al 1992;Speirs and Cutz 1993). On the other hand, cell dissociation, coculture, and explant techniques facilitate in vitro studies with systems containing multiple cell types (Ballard et al 1991;Cohn and Adler 1991;Haley et al 2008;Halldorsson et al 2007;Mangum et al 1990;Siminski et al 1992;Tosi et al 1994;Whitcutt et al 1988).…”

Oxygen‐derived radicals stimulate renin release of isolated juxtaglomerular cells

“…There is now general agreement that reactive oxygen metabolites such as 02 and H202 are continuously produced in vivo, e.g. by endothelial cells [3,4] or by activated macrophages [5]. However, formation of reactive oxygen metabolites is enhanced in inflammatory kidney diseases like glomerulonephritis or interstitial nephritis, or in post-ischemic acute renal failure [6][7][8].…”