Spin coating mediated morphology modulation in self assembly of peptides

Bhandaru, Nandini; Kaur, Gagandeep; Panjla, Apurva; Verma, Sandeep

doi:10.1039/d0nr09082d

Cited by 11 publications

(12 citation statements)

References 43 publications

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“…However, based on a rather counter-intuitive approach, in this article, we show that by adding thiol-capped gold NPs (AuNP) only to the top PS layer, we could completely stabilize a PS−PMMA bilayer, coated on a non-wettable substrate, above a critical AuNP concentration (C NP ≈ 10.0%). In our experiments, the thickness of both the layers is kept constant at ≈25 nm, and it was verified based on control experiments that single layers of both the polymers, as well as the particle-free bilayer, completely dewet on the hydrophobized silicon wafer substrate within 24…”

Section: ■ Introductionmentioning

confidence: 90%

“…A film exhibits spontaneous instability when A E > 0, and from the above relation, it can be seen that λ S scales as h F 2 . While such instability is undesirable from the standpoint of a coating, dewetting-mediated pattern formation is considered as a viable nonlithographic surface patterning technique, particularly in conjugation with suitable templating strategies, and has found a direct application in the enhancement of photoluminescence, water harvesting, − fabrication of water-stable self-powered ultraviolet (UV) detectors, self-assembly of peptides, and so on.…”

Section: Introductionmentioning

confidence: 99%

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Nanoparticle-Mediated Stabilization of a Thin Polymer Bilayer

et al. 2022

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We show that an unstable thin polymer bilayer comprising a polystyrene (PS) top and polymethyl methacrylate (PMMA) bottom layer remains completely stable on a non-wettable, hydrophobized silicon wafer substrate, even after 600 h of thermal annealing at a temperature that is higher than the glass transition temperature (T G) of both the polymers, when thiol-capped gold nanoparticles (AuNPs) are added only to the PS top layer at a concentration (C NP) ≈ 10.0% (wt. of NP/wt. of polymer). We also show that in films with C NP as low as 2.5%, the bottom PMMA layer becomes completely stabilized while the top PS layer undergoes partial dewetting, the extent of which progressively reduces with an increase in C NP. X-ray reflectivity (XRR) studies reveal that the AuNPs added to the top PS layer migrates toward the film–substrate interface within a few minutes of thermal annealing, imparting stability to the bottom PMMA layer. On the other hand, in films with a higher C NP ≈ 10.0%, particles are also seen to accumulate in the vicinity of the free surface, in addition to the film–substrate interface, resulting in complete stabilization of the bilayer. The extracted electron density profile from XRR shows that the AuNPs accumulate both at the free surface as well as at the film–substrate interfaces. Grazing incidence small-angle X-ray scattering (GISAXS) suggests the formation of an in-plane ordering of Au–polymer–Au clusters at the polymer–air and polymer–substrate interfaces with thermal annealing, which plays a pivotal role in arresting the dewetting of the bilayer by strengthening the interfaces. The novelty of the technique reported here lies in complete stabilization of an unstable bilayer by adding particles only to the top layer. This technique is likely to be helpful in obtaining stable multicomponent ultrathin films even on non-wettable surfaces.

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Section: ■ Introductionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Nanoparticle-Mediated Stabilization of a Thin Polymer Bilayer

et al. 2022

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“…For better imaging of GO flakes, spin coating (Apex Instruments) was done to avoid agglomeration and the coffee stain effect as seen in the drop cast samples. 35 Scanning Electron Microscopy (FESEM, Model: Apreo LoVac), Atomic Force Microscopy (AFM, Agilent Technologies, USA), and Optical Microscopy (OM, Metavis) was used for imaging. Spin coating was done on a cleaned silicon wafer using a 0.25% dilute dispersion of GO in tetrahydrofuran (THF) at 1000 rpm.…”

Section: Methodsmentioning

confidence: 99%

Durability studies of underwater superoleophobic graphene oxide coated wire mesh

2023

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“…Nonetheless, these molecules, also classed as nucleopeptides, are able to form supramolecular networks, cross cellular membranes and exhibit useful bioactivities [3][4][5][6][7]. Since the overall nucleopeptide properties arise from both the amino acid and nucleobase moieties, it is worth underlining that short peptides are able to selfassemble into different nanostructures [8][9][10][11], while nucleobases in synthetic molecules and especially in modified nucleosides [12][13][14][15][16] may lead to several biological properties. For example, when single nucleobases were inserted into polyamino acid chains, certain protein and peptide structures were stabilised, improving their function [17,18], while nucleobase-conjugated diphenylalanines were found to self-assemble into nanostructures useful in various biomedical applications [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Self-assembly of thyminyl l-tryptophanamide (TrpT) building blocks for the potential development of drug delivery nanosystems

et al. 2023

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The design, synthesis and characterization of a novel nucleoamino acid derivative based on an l-tryptophanamide functionalised with a thymine nucleobase (named TrpT) is here described. The novel construct’s tendency to self-assemble into supramolecular networks in aqueous solution was demonstrated by dynamic light scattering (DLS), circular dichroism (CD), fluorescence and UV spectroscopic measurements. TrpT nanoaggregates showed good stability (up to 5 h) at 140 µM and proved to comprise species of mean hydrodynamic diameter 330 nm and a homogeneous size distribution; scanning electron microscopy (SEM) analysis further revealed these to be spherical-shaped assemblies. The ability of TrpT nanoaggregates to bind curcumin, selected as a model anticancer drug, was also evaluated and its release was monitored over time by confocal microscopy. Molecular docking studies were performed on both TrpT self-assembly and curcumin-loaded nanoaggregates suggesting that the phytomolecule can be accommodated in the interior of the supramolecular network via hydrophobic (π−π and π-alkyl) interactions; the formation of TrpT-curcumin adducts may improve the polarity of the highly-hydrophobic curcumin with a resulting logP closer to the optimal values expected for a good drug bioavailability, as estimated by the ADMETlab software. Finally, the high stability of TrpT nanoassembly in human serum, and the absence of significant toxic effects on human model cells in a cell viability assay, were also demonstrated. Despite its thymine-based scaffold, TrpT was shown not to bind adenine-bearing nucleic acids, suggesting that this interaction is hindered by its intrinsic propensity to self-assemble in preference to forming A-T base pairings. Instead, TrpT was able to interact with a serum protein such as bovine serum albumin (BSA), known to improve the bloodstream transportation and bioavailability of its cargo. Collectively, our findings support the potential use of TrpT for the development of new drug delivery systems. Graphical abstract

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Spin coating mediated morphology modulation in self assembly of peptides

Abstract: A novel strategy for modulating the self-assembled morphology of diphenylalanine peptides based on centrifugal force mediated spin coating and spin dewetting triggered by disjoining pressure in ultra-thin films during rotation.

Cited by 11 publications

References 43 publications

Nanoparticle-Mediated Stabilization of a Thin Polymer Bilayer

Nanoparticle-Mediated Stabilization of a Thin Polymer Bilayer

Durability studies of underwater superoleophobic graphene oxide coated wire mesh

Self-assembly of thyminyl l-tryptophanamide (TrpT) building blocks for the potential development of drug delivery nanosystems

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