Spider webs capture environmental DNA from terrestrial vertebrates

Newton, Joshua P.; Nevill, Paul; Bateman, Philip W.; Campbell, Matthew A.; Allentoft, Morten E.

doi:10.1016/j.isci.2024.108904

Cited by 3 publications

(1 citation statement)

References 61 publications

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“…The existing eDNA studies of wild bird species include determining the distribution of the North American marshland bird, the black rail ( Laterallus jamaicensis ) (Feist et al., 2022 ; Neice & McRae, 2021 ) and the Ridgway's rail ( Rallus obsoletus ) (Guan et al., 2023 ), detecting an endangered land bird, the Gouldian finch ( Chloebia gouldiae ), from drinking sites (Day et al., 2019 ), as well as detecting the kākāpō ( Strigops habroptilus ) from soil samples (Urban et al., 2023 ). Furthermore, studies have focused on detecting various avian species in diverse habitats, including urban waterbodies (Zhang et al., 2023 ), natural wetlands (Coleman et al., 2023 ), leaf swaps (Lynggaard et al., 2023 ), airborne dust (Johnson et al., 2023 ), air (Lynggaard et al., 2024 ), and spider webs (Newton et al., 2024 ). Additionally, several bird pollinators and an insectivorous bird were identified from eDNA collected from flowers (Jønsson et al., 2023 ; Newton et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Optimised PCR assays for detecting elusive waterfowl from environmental DNA

Honka,

Kvist,

Olli

et al. 2024

Ecology and Evolution

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For many aquatic and semiaquatic mammal, amphibian and fish species, environmental DNA (eDNA) methods are employed to detect species distribution and to monitor their presence, but eDNA is much less employed for avian species. Here, we developed primers for the detection of true geese and swan species using eDNA and optimised a PCR protocol for eDNA. We selected taiga bean goose (Anser fabalis fabalis) as our focal (sub)species and sampled water from lakes, from which the presence of taiga bean goose was visually confirmed. To test, if taiga bean goose DNA could be detected among DNA of other goose species, we similarly sampled eDNA from a zoo pond housing several Anatidae species. We were able to detect taiga bean goose DNA in all but one of the tested lakes, including the zoo pond. The primers developed are not species‐specific, but rather specific to the genus Anser, due to the close relatedness of Anser species, which prevented the development of species‐specific primers and the use of, for example, quantitative PCR. We also developed eDNA primers for Branta species and Cygnus species and tested these primers using the same samples. Canada goose (B. canadensis) and barnacle goose (B. leucopsis) DNA were only detected in the zoo pond (in which they were present), as the sampled natural lakes fall outside the range of these species. We detected whooper swan (C. cygnus) DNA in three lakes and the zoo pond (in which the species was present). The eDNA method presented here provides a potential means to monitor elusive goose species and to study the co‐occurrence of large waterfowl.

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Section: Introductionmentioning

confidence: 99%

Optimised PCR assays for detecting elusive waterfowl from environmental DNA

Honka,

Kvist,

Olli

et al. 2024

Ecology and Evolution

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Continuous daily sampling of airborne eDNA detects all vertebrate species identified by camera traps

Polling,

Buij,

Laros

et al. 2024

Environmental DNA

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Ongoing pressures on global biodiversity require conservation action that is not possible without effective biomonitoring. Terrestrial vertebrate surveys are commonly performed using camera traps, a time‐intensive method known to miss many small or arboreal species and birds. Recent advances have shown airborne eDNA to be a potentially suitable technique to more effectively monitor vertebrate communities in a time‐ and cost‐effective manner. Here, we test whether commercially available air samplers that collect air particles 24/7 during a 1‐week period can be used to detect the presence of vertebrates through airborne eDNA. The results are compared to camera trap records at three locations with differing habitats in the Netherlands. Simultaneous sampling with three different air samplers for 3 weeks resulted in detection of 154 vertebrate taxa, of which the majority were birds or mammals (113 and 33 species, respectively), along with four fish and four amphibian species. All species observed using camera traps were also retrieved via airborne eDNA, although not on every day of sampling. The Burkard spore trap, used routinely for pollen monitoring, showed the highest number of vertebrate species, and only in three samples when a mammal species was detected using a camera trap it remained undetected via eDNA. We also detected unique species at the three locations using airborne eDNA, indicative of the habitat in which they were living. However, we also detected species that we could not account for. The multitude of species found using airborne eDNA compared to camera traps indicate the sensitivity of the method; however, subsequent studies should prioritize validation of these findings through alternative biomonitoring approaches.

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Identification of orb-web spider species and their food source through environmental DNA analysis

Kim,

Kim

2024

J Ecol Environ

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Spider webs capture environmental DNA from terrestrial vertebrates

Cited by 3 publications

References 61 publications

Optimised PCR assays for detecting elusive waterfowl from environmental DNA

Optimised PCR assays for detecting elusive waterfowl from environmental DNA

Continuous daily sampling of airborne eDNA detects all vertebrate species identified by camera traps

Identification of orb-web spider species and their food source through environmental DNA analysis

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