Spider mite egg extract modifies Arabidopsis response to future infestations

Ojeda-Martinez, Dairon; Martínez, Manuel; Dı́az, Isabel; Santamaría, M. Estrella

doi:10.1038/s41598-021-97245-z

Cited by 8 publications

(10 citation statements)

References 108 publications

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“…In the case of the lepidopteran, the pre-exposition to eggs or egg extract has had mixed results on the feeding pest stage (Gouhier-Darimont et al, 2013;Lortzing et al, 2019). Although the plant pre-exposition to egg extract in the case of the spider mite has stimulated the feeding behavior of the adults (Ojeda-Martinez et al, 2021). The aforementioned evidence could indicate a priming effect of the oviposition stress, which either warns the plant or tricks its defenses to enhance the success of the feeding pest.…”

Section: Timeline Of Functional Processesmentioning

confidence: 99%

“…The application of PCs on plants triggered SA accumulation and immune responses similar to natural oviposition. Unlike its counterpart, T. urticae egg extract involved JA cascades, among other processes (Ojeda-Martinez et al, 2021). This might lead to think that species-specific elicitors could be interacting with the plant, of which P. brassicae's are undoubtedly inducing a more robust response.…”

Section: Gene Co-expression Network Analysismentioning

confidence: 99%

“…Nonetheless, the stresses by both species share their negative impact on the processes involving future generations. Pieris oviposition activates defenses detrimental for feeding larvae (Lortzing et al, 2019), and Tetranychus egg extract induces defense mechanisms that reduce female fertility (Ojeda-Martinez et al, 2021).…”

Section: Gene Co-expression Network Analysismentioning

confidence: 99%

“…All relevant supporting datasets are included in the article and Supplementary Material. The original studies can be found on the following repositories: National Center for Biotechnology Information (NCBI) BioProject database; T. urticae RNAseq (Ojeda-Martinez et al, 2021) under the accession code GSE168993; P. bassicae 72 h Ov RNA-seq (Nallu et al, 2018) under the accession code SRP134094; P. bassicae 120 h Ov and Ex RNA-seq (Stahl et al, 2020) under the accession code SRP244078; P. bassicae 120 h Ov Microarray (Firtzlaff et al, 2016) under the accession code GSE69623; and P. bassicae 144 h Ov Microarray (Lortzing et al, 2019) under the accession code GSE114041. Link to PPI networks and steps followed to assemble them: https: //www.cbgp.upm.es/files/Network_Analysis.php.…”

Section: Data Availability Statementmentioning

confidence: 99%

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Transcriptomic Landscape of Herbivore Oviposition in Arabidopsis: A Systematic Review

2022

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Herbivore oviposition produces all sorts of responses in plants, involving wide and complex genetic rearrangements. Many transcriptomic studies have been performed to understand this interaction, producing a bulk of transcriptomic data. However, the use of many transcriptomic techniques across the years, the lack of comparable transcriptomic context at the time of publication, and the use of outdated databases are limitations to understand this biological process. The current analysis intends to retrieve oviposition studies and process them with up-to-date techniques and updated databases. To reduce heterogeneities, the same processing techniques were applied, and Arabidopsis was selected to avoid divergencies on plant taxa stress response strategies. By doing so, we intended to understand the major mechanisms and regulatory processes linked to oviposition response. Differentially expressed gene (DEG) identification and co-expression network-based analyses were the main tools to achieve this goal. Two microarray studies and three RNA-seq analyses passed the screening criteria. The collected data pertained to the lepidopteran Pieris brassicae and the mite Tetranychus urticae, and covered a timeline from 3 to 144 h. Among the 18, 221 DEGs found, 15, 406 were exclusive of P. brassicae (72 h) and 801 were exclusive for the rest of the experiments. Excluding P. brassicae (72 h), shared genes on the rest of the experiments were twice the unique genes, indicating common response mechanisms were predominant. Enrichment analyses indicated that shared processes were circumscribed to earlier time points, and after 24 h, the divergences escalated. The response was characterized by patterns of time-dependent waves of unique processes. P. brassicae oviposition induced a rich response that shared functions across time points, while T. urticae eggs triggered less but more diverse time-dependent functions. The main processes altered were associated with hormonal cascades [e.g., salicilic acid (SA) and jasmonic acid (JA)], defense [reactive oxygen species (ROS) and glucosinolates], cell wall rearrangements, abiotic stress responses, and energy metabolism. Key gene drivers of the identified processes were also identified and presented. The current results enrich and clarify the information regarding the molecular behavior of the plant in response to oviposition by herbivores. This information is valuable for multiple stress response engineering tools, among other applications.

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Section: Timeline Of Functional Processesmentioning

confidence: 99%

Section: Gene Co-expression Network Analysismentioning

confidence: 99%

Section: Gene Co-expression Network Analysismentioning

confidence: 99%

Section: Data Availability Statementmentioning

confidence: 99%

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Transcriptomic Landscape of Herbivore Oviposition in Arabidopsis: A Systematic Review

2022

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“…Moreover, a portion of the transcriptional response to eggs appears to be conserved between different plant-insect egg interactions (Lortzing et al 2020). Components of the plant immune response to eggs seem to be shared across unrelated plant species as, for example, the accumulation of ROS (de Puysseleyr et al 2011;Kim et al 2012;Gouhier-Darimont et al 2013;Bittner et al 2017;Geuss et al 2017;Das et al 2021;Oates et al 2021;Ojeda-Martinez et al 2021). However, different phytohormonal pathways may be specifically induced by different interactions.…”

Section: Plant Responses To Insect Eggsmentioning

confidence: 99%

E(gg)xit strategy of plant defense : Evolution and genetics of a butterfly egg-triggered cell death

Bassetti

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General introduction17 General introduction 17 in B. nigra a cell death and PR1 gene expression, a plant immunity marker. Finally, few Brassicaceae species were selected to study the effect of cell death severity on eggs survival.Chapter 3 describes different plant immunity responses associated with HR-like cell death in a crop (B. rapa) and a wild relative (B. nigra). First, I investigated types and timing of the immune responses developed by the two Brassica species. Then, I used these responses to characterize the difference between eggs of P. brassicae, a brassicaceaous specialist, with eggs of the generalist moth Mamestra brassicae. B. nigra accessions with contrasting HR-like phenotypes were tested for their ability to express a canonical HR against pathogens. Further, gene expression of SA-and JA-related defence markers was explored in these B. nigra accessions to link variation in egg-induced cell death to different regulation of defense-related phytohormones.Chapter 4 investigates the strength of HR-like cell death within the crop species B. rapa. A subset of 56 B. rapa accessions derived from a core collection was screened to explore the diversity and variation in cell death. I developed an image-based phenotyping protocol to quantify cell death size in an accurate and portable manner. This phenotyping protocol was used to identify two accessions with contrasting cell death phenotype and to screen a RIL population to perform QTL mapping. Finally, syntenic relationships between the QTLs that I identified and A. thaliana were analysed.Chapter 5 studies the genetic basis of HR-like cell death in B. nigra accessions by crossing plants from a local wild population. First, investigated the inheritance of the trait using different types of crosses. As I expected a simple genetic architecture, I performed genetic mapping using bulk-segregant analysis (BSA) coupled with whole-genome sequencing (BSA-seq). The locus identified with BSA-seq was validated with molecular markers and fine-mapped through recombinant analysis. Next, differential gene expression at the locus region between the two parents was assessed through RNA sequencing. Further, I explored the genetic variation at the locus between the parental accessions and among the available B. nigra genomes. Finally, I discussed implications for further fine-mapping of the locus.Chapter 6 provides a general discussion that places my thesis into a broader context by comparing my results with the literature available. In this final chapter, I speculate how my results could provide a mechanism for perception of Pieris eggs by the plant immune system, I cover the implication for plant-insect coevolution and, finally, I discuss perspectives for future research.

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