Spider Glue Proteins Have Distinct Architectures Compared with Traditional Spidroin Family Members

Vasanthavada, Keshav; Hu, Xiaoyi; Tuton-Blasingame, Tiffany; Hsia, Yang; Sampath, Sujatha; Pacheco, Ryan; Freeark, Jordan; Falick, Arnold M.; Tang, Simon Y.; Fong, Justine; Köhler, Kristin; Mattina-Hawkins, Coby La; Vierra, Craig

doi:10.1074/jbc.m112.399816

Cited by 40 publications

(57 citation statements)

References 37 publications

(36 reference statements)

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“…Cement collections display a matted, nanofibrillar ultrastructure1213495051, that forms in both sealed and porous attachment conditions, and is readily dissolved by polar solvents used to solubilize biomaterials rich in hydrogen bonds and β-sheet structures525354. Relative abundance of proteins in MS/MS data confirms PAGE observations, where Aa43 and polar GSrCPs occupy a significant fraction of cement.…”

Section: Discussionsupporting

confidence: 64%

Sequence basis of Barnacle Cement Nanostructure is Defined by Proteins with Silk Homology

Fears

Leary

et al. 2016

Sci Rep

194

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Barnacles adhere by producing a mixture of cement proteins (CPs) that organize into a permanently bonded layer displayed as nanoscale fibers. These cement proteins share no homology with any other marine adhesives, and a common sequence-basis that defines how nanostructures function as adhesives remains undiscovered. Here we demonstrate that a significant unidentified portion of acorn barnacle cement is comprised of low complexity proteins; they are organized into repetitive sequence blocks and found to maintain homology to silk motifs. Proteomic analysis of aggregate bands from PAGE gels reveal an abundance of Gly/Ala/Ser/Thr repeats exemplified by a prominent, previously unidentified, 43 kDa protein in the solubilized adhesive. Low complexity regions found throughout the cement proteome, as well as multiple lysyl oxidases and peroxidases, establish homology with silk-associated materials such as fibroin, silk gum sericin, and pyriform spidroins from spider silk. Distinct primary structures defined by homologous domains shed light on how barnacles use low complexity in nanofibers to enable adhesion, and serves as a starting point for unraveling the molecular architecture of a robust and unique class of adhesive nanostructures.

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Section: Discussionsupporting

confidence: 64%

Sequence basis of Barnacle Cement Nanostructure is Defined by Proteins with Silk Homology

Fears

Leary

et al. 2016

Sci Rep

194

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“…It is the glycoprotein composition that primarily determines stickiness in orb web gluey silks (Sahni et al, 2014b). Henceforth the differential expression of aggregate glycoprotein (Choresh et al, 2009;Vasanthavada et al, 2012;Collin et al, 2016) remains a plausible alternative explanation for the differences in spiral stickiness between spiders in the cricket and cockroach feeding treatments. Spread and extension of the glycoproteins under load is, nevertheless, enhanced if the glycoproteins are sufficiently hydrated (Stellwagen et al 2014;Opell et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Diet-induced covariation between architectural and physicochemical plasticity in an extended phenotype

Blamires

Hasemore

Martens

et al. 2016

Journal of Experimental Biology

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The adaptive benefits of extended phenotypic plasticity are imprecisely defined due to a paucity of experiments examining traits that are manipulable and measurable across environments. Spider webs are often used as models to explore the adaptive benefits of variations in extended phenotypes across environments. Nonetheless, our understanding of the adaptive nature of the plastic responses of spider webs is impeded when web architectures and silk physicochemical properties appear to co-vary. An opportunity to examine this co-variation is presented by modifying prey items while measuring web architectures and silk physiochemical properties. Here, we performed two experiments to assess the nature of the association between web architectures and gluey silk properties when the orb web spider Argiope keyserlingi was fed a diet that varied in either mass and energy or prey size and feeding frequency. We found web architectures and gluey silk physicochemical properties to co-vary across treatments in both experiments. Specifically, web capture area co-varied with gluey droplet morphometrics, thread stickiness and salt concentrations when prey mass and energy were manipulated, and spiral spacing co-varied with gluey silk salt concentrations when prey size and feeding frequency were manipulated. We explained our results as A. keyserlingi plastically shifting its foraging strategy as multiple prey parameters simultaneously varied. We confirmed and extended previous work by showing that spiders use a variety of prey cues to concurrently adjust web and silk traits across different feeding regimes.

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“…Biochemical studies demonstrated that the SCP-1 peptide has intrinsic metal binding properties, suggesting a role of peptide-metal ion interactions with the fiber constituents to enhance thread performance [44]. In L. hesperus the aggregate gland has also been shown to produce two different proteins, aggregate gland silk factor 1 (AgSF1) and aggregate gland silk factor 2 (AgSF2) [47]. These proteins were found to be co-localized to the connection joints of both webs and wrapping silks spun from the black widow spider.…”

Section: Spider Silk Types and Their Modulesmentioning

confidence: 99%

“…In case of AgSF2, the consensus novel repetitive sequence, NVNVN, was determined. The amino acid block repeats were found to be embedded in a glycine-rich matrix of AgSF2 [47]. Adhesive spider silk proteins (glues) are important members of the silk family that allow spiders to perform a variety of tasks, such as web construction, prey capture, and locomotion.…”

Section: Spider Silk Types and Their Modulesmentioning

confidence: 99%

Structure–function–property–design interplay in biopolymers: Spider silk

et al. 2014

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Spider silks have been a focus of research for almost two decades due to their outstanding mechanical and biophysical properties. Recent advances in genetic engineering have led to the synthesis of recombinant spider silks, thus helping to unravel a fundamental understanding of structure-function-property relationships. The relationships between molecular composition, secondary structures, and mechanical properties found in different types of spider silks are described, along with a discussion of artificial spinning of these proteins and their bioapplications, including the role of silks in biomineralization and fabrication of biomaterials with controlled properties.

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Spider Glue Proteins Have Distinct Architectures Compared with Traditional Spidroin Family Members

Cited by 40 publications

References 37 publications

Sequence basis of Barnacle Cement Nanostructure is Defined by Proteins with Silk Homology

Sequence basis of Barnacle Cement Nanostructure is Defined by Proteins with Silk Homology

Diet-induced covariation between architectural and physicochemical plasticity in an extended phenotype

Structure–function–property–design interplay in biopolymers: Spider silk

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