Spider Chitin: An Ultrafast Microwave-Assisted Method for Chitin Isolation from Caribena versicolor Spider Molt Cuticle

Machałowski, Tomasz; Wysokowski, Marcin; Tsurkan, Mikhail V.; Galli, Roberta; Schimpf, Christian; Rafaja, David; Brendler, Erica; Viehweger, Christine; Żółtowska-Aksamitowska, Sonia; Petrenko, Iaroslav; Czaczyk, Katarzyna; Kraft, Michael; Bertau, Martin; Bechmann, Nicole; Guan, Kaomei; Bornstein, Stefan R.; Voronkina, Alona; Fursov, Andriy; Bejger, Magdalena; Biniek-Antosiak, Katarzyna; Rypniewski, W.; Figlerowicz, Marek; Pokrovsky, Oleg S.; Jesionowski, Teofil; Ehrlich, Hermann

doi:10.3390/molecules24203736

Cited by 35 publications

(33 citation statements)

References 93 publications

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“…Chitin is an evolutionarily ancient and superior scaffolding biomaterial which offers nanofibrous framework for mechanical support and imparts to the skeletons of invertebrate species and biological systems across the globe [2,[44][45][46][47][48]. Chemically, chitin is an insoluble, nanocrystalline linear polysaccharide of β-1,4-linked N-acetylglucosamine units that occurs in three polymorphic forms, namely alfa-, beta-and gamma-chitin [49].…”

Section: Chitin Scaffoldsmentioning

confidence: 99%

Modern scaffolding strategies based on naturally pre-fabricated 3D biomaterials of poriferan origin

et al. 2020

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Modern scaffolding strategies include two key ways: to produce requested 3D constructs from corresponding precursors using technological tools, or simply use naturally already prefabricated scaffolds if they originate from renewable sources. Marine sponges inhabit oceans since the Precambrian. These ancient multicellular organisms possess a broad variety of evolutionary approved and ready to use skeletal structures, which seem to be well applicable as 3D scaffolds in diverse fields of modern bioinspired materials science, biomimetics and regenerative medicine. In this review, most attention is paid to biosilica-, chitin-, and spongin-based scaffolds of poriferan origin with respect to their potential use.

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Section: Chitin Scaffoldsmentioning

confidence: 99%

Modern scaffolding strategies based on naturally pre-fabricated 3D biomaterials of poriferan origin

et al. 2020

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“…Mar. Drugs 2020, 18, 297; doi:10.3390/md18060297 www.mdpi.com/journal/marinedrugs This most abundant aminopolysaccharide has been isolated and identified in skeletal structures of diverse species of fungi, algae and invertebrates (i.e., sponges, hydrozoans, mollusks, worms, insects, spiders and crustaceans) [2][3][4][5][6][7][8][9][10][11][12]. Here, chitin is present in the form of biocomposites, being chemically bound to proteins, pigments and other polysaccharides, as well as mineral phases [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…Regardless, in order to increase efficiency and reduce the environmental impact of chemical the process, novel and modified methods have been developed [22][23][24]. The recently reported assisted methods are mostly focused on the use of microwave irradiation as the accelerating factor [9]. Indeed, the application of this approach leads to a significant reduction of treatment time (from days to a few hours) [15].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia)

et al. 2020

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The development of novel and effective methods for the isolation of chitin, which remains one of the fundamental aminopolysaccharides within skeletal structures of diverse marine invertebrates, is still relevant. In contrast to numerous studies on chitin extraction from crustaceans, mollusks and sponges, there are only a few reports concerning its isolation from corals, and especially black corals (Antipatharia). In this work, we report the stepwise isolation and identification of chitin from Cirrhipathes sp. (Antipatharia, Antipathidae) for the first time. The proposed method, aiming at the extraction of the chitinous scaffold from the skeleton of black coral species, combined a well-known chemical treatment with in situ electrolysis, using a concentrated Na2SO4 aqueous solution as the electrolyte. This novel method allows the isolation of α-chitin in the form of a microporous membrane-like material. Moreover, the extracted chitinous scaffold, with a well-preserved, unique pore distribution, has been extracted in an astoundingly short time (12 h) compared to the earlier reported attempts at chitin isolation from Antipatharia corals.

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“…Therefore, this method is often modified in order to reduce treatment time and the amount of chemicals that are used. Recently reported methods of chitin extraction are mostly focused on the use of microwave irradiation as accelerating factor [43,44].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

et al. 2020

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Three-dimensional (3D) biopolymer-based scaffolds including chitinous matrices have been widely used for tissue engineering, regenerative medicine and other modern interdisciplinary fields including extreme biomimetics. In this study, we introduce a novel, electrochemically assisted method for 3D chitin scaffolds isolation from the cultivated marine demosponge Aplysina aerophoba which consists of three main steps: (1) decellularization, (2) decalcification and (3) main deproteinization along with desilicification and depigmentation. For the first time, the obtained electrochemically isolated 3D chitinous scaffolds have been further biomineralized ex vivo using hemolymph of Cornu aspersum edible snail aimed to generate calcium carbonates-based layered biomimetic scaffolds. The analysis of prior to, during and post-electrochemical isolation samples as well as samples treated with molluscan hemolymph was conducted employing analytical techniques such as SEM, XRD, ATR-FTIR and Raman spectroscopy. Finally, the use of described method for chitin isolation combined with biomineralization ex vivo resulted in the formation of crystalline (calcite) calcium carbonate-based deposits on the surface of chitinous scaffolds, which could serve as promising biomaterials for the wide range of biomedical, environmental and biomimetic applications.

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Spider Chitin: An Ultrafast Microwave-Assisted Method for Chitin Isolation from Caribena versicolor Spider Molt Cuticle

Cited by 35 publications

References 93 publications

Modern scaffolding strategies based on naturally pre-fabricated 3D biomaterials of poriferan origin

Modern scaffolding strategies based on naturally pre-fabricated 3D biomaterials of poriferan origin

Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia)

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

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