Sphingosylphosphorylcholine induces stress fiber formation via activation of Fyn-RhoA-ROCK signaling pathway in fibroblasts

Xu, Dan; Kishi, Hiroko; Kawamichi, Hozumi; Kajiya, Katsuko; Takada, Yuichi; Kobayashi, Sei

doi:10.1016/j.cellsig.2011.09.013

Cited by 18 publications

(29 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considerable evidence indicates that RhoA-ROCK pathway signals the reorganization of the actin cytoskeleton, which induces the formation of stress fibers [17,18]. To address the possibility that the stress fiber alteration of AAPE treated HK is involved in RhoA-ROCK signaling, we checked the level of RhoA-GDP/GTP exchange activity with HK lysates.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Secretory Factors of Adipose-Derived Stem Cells on Human Keratinocytes

Moon

Park²,

Lee

et al. 2012

IJMS

107

View full text Add to dashboard Cite

Abstract:The beneficial effects of adipose-derived stem cell conditioned medium (ADSC-CM) on skin regeneration have been reported. Although the mechanism of how ADSC-CM promotes skin regeneration is unclear, ADSC-CM contained various growth factors and it is an excellent raw material for skin treatment. ADSC-CM produced in a hypoxia condition of ADSC-in other words, Advanced Adipose-Derived Stem cell Protein Extract (AAPE)-has great merits for skin regeneration. In this study, human primary keratinocytes (HKs), which play fundamental roles in skin tissue, was used to examine how AAPE affects HK. HK proliferation was significantly higher in the experimental group (1.22 μg/mL) than in the control group. DNA gene chip demonstrated that AAPE in keratinocytes (p < 0.05) notably affected expression of 290 identified transcripts, which were associated with cell proliferation, cycle and migration. More keratinocyte wound healing and migration was shown in the experimental group (1.22 μg/mL). AAPE treatment significantly stimulated stress fiber formation, which was linked to the OPEN ACCESS Int. J. Mol. Sci. 2012, 13 1240RhoA-ROCK pathway. We identified 48 protein spots in 2-D gel analysis and selected proteins were divided into 64% collagen components and 30% non-collagen components as shown by the MALDI-TOF analysis. Antibody array results contained growth factor/cytokine such as HGF, FGF-1, G-CSF, GM-CSF, IL-6, VEGF, and TGF-β3 differing from that shown by 2-D analysis. Conclusion: AAPE activates HK proliferation and migration. These results highlight the potential of the topical application of AAPE in the treatment of skin regeneration.

show abstract

Section: Resultsmentioning

confidence: 99%

The Effect of Secretory Factors of Adipose-Derived Stem Cells on Human Keratinocytes

Moon

Park²,

Lee

et al. 2012

IJMS

107

View full text Add to dashboard Cite

show abstract

“…It was reported that miR‐125a‐3p directly targeted RhoA mRNA in the A549 lung cancer cell line; a decrease in miR‐125a‐3p was associated with an increase in RhoA protein and with cell migration (35). However, the activity state of RhoA in fibroblasts was found to be Fyn dependent (36). Hence, though we cannot rule out the involvement of RhoA in miR‐125a‐3p‐mediated inhibition of granulosa cell migration, this pathway may also be Fyn dependent.…”

Section: Discussionmentioning

confidence: 99%

A novel regulatory pathway in granulosa cells, the LH/human chorionic gonadotropin-microRNA-125a-3p-Fyn pathway, is required for ovulation

et al. 2015

View full text Add to dashboard Cite

Granulosa cells support the developing oocytes and serve as transducers of the ovulatory stimulus induced by LH surge. Fyn kinase is expressed in granulosa cells, though its role in these cells has not been studied. In human embryonic kidney 293T cells, microRNA (miR)-125a-3p down-regulates Fyn expression, causing a decrease in cells' migratory ability. Our aim was to explore the role of miR-125a-3p and Fyn in granulosa cells toward ovulation, focusing on migration as a possible mechanism. We demonstrate expression of miR-125a-3p and Fyn in mouse mural granulosa cells of preovulatory follicles and miR125a-3p-induced down-regulation of Fyn expression in a granulosa cell line (rat). Administration of human chorionic gonadotropin (hCG; LH analog) caused a 75% decrease in the in vivo miR-125a-3p:Fyn mRNA ratio, followed by a 2-fold increased migratory ability of mural granulosa cells. In the hCG-treated granulosa cell line, miR-125a-3p expression was decreased, followed by Fyn up-regulation and phosphorylation of focal adhesion kinase and paxillin, enabling cell migration. An in vivo interference with miR125a-3p:Fyn mRNA ratio in granulosa cells by intrabursal injections of Fyn small interfering RNA or miR-125a-3p mimic caused a 33 or 55% decrease in the number of ovulated oocytes, respectively. These observations reveal a new regulatory pathway in mural granulosa cells under the regulation of LH/hCG. Modulation of cell migration may account for the significance of the LH/hCG-miR-125a-3p-Fyn pathway to ovulation.-Grossman, H., Chuderland, D., Ninio-Many, L., Hasky, N., Kaplan-Kraicer, R., Shalgi, R. A novel regulatory pathway in granulosa cells, the LH/human chorionic gonadotropin-microRNA-125a-3p-Fyn pathway, is required for ovulation. FASEB J. 29, 3206-3216 (2015). www.fasebj.org

show abstract

“…TrioGEFD2 is known as upstream of RhoA and RhoA targeted signaling [24], [25]. Activation of RhoA and RhoA targeted signaling promotes actin stress fiber formation [26], [27], leading to phosphorylation of MLC and activation of ROCK [26], [27]. These data suggest that Echo30 infection induces actin stress fiber formation through the activation of TrioGEFD2-RhoA signaling and finally results in neuronal cell death.…”

Section: Resultsmentioning

confidence: 92%

“…We presumed GEFD2-mediated activation of RhoA and RhoA targeted signaling, such as Rho-associated protein kinase (ROCK) and myosin-light chain (MLC) which are involved in Echo30 induced neuronal cell death. RhoA signaling is known to promote actin stress fiber formation [26], [27]. We predicted GEFD2 leading to actin stress fiber formation via activation of RhoA signaling.…”

Section: Introductionmentioning

confidence: 88%

Echovirus 30 Induced Neuronal Cell Death through TRIO-RhoA Signaling Activation

et al. 2012

View full text Add to dashboard Cite

BackgroundEchovirus 30 (Echo30) is one of the most frequently identified human enteroviruses (EVs) causing aseptic meningitis and encephalitis. However the mechanism underlying the pathogenesis of Echo30 infection with significant clinical outcomes is not completely understood. The aim of this investigation is to illustrate molecular pathologic alteration in neuronal cells induced by Echo30 infection using clinical isolate from young patient with neurologic involvement.Methodology/Principal FindingsTo characterize the neuronal cellular response to Echo30 infection, we performed a proteomic analysis based on two-dimensional gel electrophoresis (2-DE) and MALDI-TOF/TOF Mass Spectrophotometric (MS) analysis. We identified significant alteration of several protein expression levels in Echo30-infected SK-N-SH cells. Among these proteins, we focused on an outstanding up-regulation of Triple functional domain (TRIO) in Echo30-infected SK-N-SH cells. Generally, TRIO acts as a key component in the regulation of axon guidance and cell migration. In this study, we determined that TRIO plays a role in the novel pathways in Echo30 induced neuronal cell death.Conclusions/SignificanceOur finding shows that TRIO plays a critical role in neuronal cell death by Echo30 infection. Echo30 infection activates TRIO-guanine nucleotide exchange factor (GEF) domains (GEFD2) and RhoA signaling in turn. These results suggest that Echo30 infection induced neuronal cell death by activation of the TRIO-RhoA signaling. We expect the regulation of TRIO-RhoA signaling may represent a new therapeutic approach in treating aseptic meningitis and encephalitis induced by Echo30.

show abstract

Sphingosylphosphorylcholine induces stress fiber formation via activation of Fyn-RhoA-ROCK signaling pathway in fibroblasts

Cited by 18 publications

References 35 publications

The Effect of Secretory Factors of Adipose-Derived Stem Cells on Human Keratinocytes

The Effect of Secretory Factors of Adipose-Derived Stem Cells on Human Keratinocytes

A novel regulatory pathway in granulosa cells, the LH/human chorionic gonadotropin-microRNA-125a-3p-Fyn pathway, is required for ovulation

Echovirus 30 Induced Neuronal Cell Death through TRIO-RhoA Signaling Activation

Contact Info

Product

Resources

About