Sphingosylphosphorylcholine-induced interleukin-6 production is mediated by protein kinase C and p42/44 extracellular signal-regulated kinase in human dermal fibroblasts

Kwon, Yoo Bin; Lee, Youngsook; Sohn, Kyung-Ah; Piao, Yongjun; Back, Seung Ju; Seo, Young‐Joon; Suhr, Ki-Beom; Park, Jang-Kyu; Kim, Chang Deok; Lee, Jeung‐Hoon

doi:10.1016/j.jdermsci.2007.01.007

Cited by 14 publications

(9 citation statements)

References 37 publications

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“…In our system, the physical forces applied to the cells did not influence this enzyme. In many systems, PKC-alpha activates the gene expression of IL-6 [36], [37], which is a multifunctional cytokine expressed by human thyrocytes [38], [39]. We observed that IL6 expression was modified, while PKC-alpha gene expression remained unaffected.…”

Section: Discussionmentioning

confidence: 78%

Interleukin-6 Expression under Gravitational Stress Due to Vibration and Hypergravity in Follicular Thyroid Cancer Cells

Wehland

Aleshcheva

et al. 2013

PLoS ONE

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It is known that exposing cell lines in vitro to parabolic flights changes their gene expression and protein production patterns. Parabolic flights and spaceflight in general are accompanied by transient hypergravity and vibration, which may impact the cells and therefore, have to be considered too. To estimate the possible impact of transient hypergravity and vibration, we investigated the effects of these forces separately using dedicated ground-based facilities. We placed follicular thyroid ML-1 and CGTH W-1 cancer cells in a specific centrifuge (MuSIC Multi Sample Incubator Centrifuge; SAHC Short Arm Human Centrifuge) simulating the hypergravity phases that occur during one (P1) and 31 parabolas (P31) of parabolic flights, respectively. On the Vibraplex device, the same cell lines were treated with vibration waves corresponding to those that occur during a whole parabolic flight lasting for two hours. After the various treatments, cells were harvested and analyzed by quantitative real-time PCR, focusing on the genes involved in forming (ACTB, MYO9, TUBB, VIM, TLN1, and ITGB1) and modulating (EZR, RDX, and MSN) the cytoskeleton, as well as those encoding growth factors (EGF, CTGF, IL6, and IL8) or protein kinases (PRKAA1 and PRKCA). The analysis revealed alterations in several genes in both cell lines; however, fewer genes were affected in ML-1 than CGTH W-1 cells. Interestingly, IL6 was the only gene whose expression was changed in both cell lines by each treatment, while PKCA transcription remained unaffected in all experiments. We conclude that a PKCa-independent mechanism of IL6 gene activation is very sensitive to physical forces in thyroid cells cultured in vitro as monolayers.

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Section: Discussionmentioning

confidence: 78%

Interleukin-6 Expression under Gravitational Stress Due to Vibration and Hypergravity in Follicular Thyroid Cancer Cells

Wehland

Aleshcheva

et al. 2013

PLoS ONE

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“…For example, sphingosine-1-phosphate, produced by platelets at the haemostasis phase of wound healing, has been shown to promote keratinocyte migration and wound healing [ 112 – 114 ]. Sphingosylphosphorylcholine increases proliferation of human keratinocytes, and induces the production of wound healing factors by human fibroblasts, e.g., connective growth tissue factor, IL-6 and plasminogen activator inhibitor-1 [ 115 – 118 ].…”

Section: Factors Regulating the Transition From Inflammation To Prolimentioning

confidence: 99%

Transition from inflammation to proliferation: a critical step during wound healing

Landén

Ståhle

2016

Cell. Mol. Life Sci.

1,205

982

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The ability to rapidly restore the integrity of a broken skin barrier is critical and is the ultimate goal of therapies for hard-to-heal-ulcers. Unfortunately effective treatments to enhance healing and reduce scarring are still lacking. A deeper understanding of the physiology of normal repair and of the pathology of delayed healing is a prerequisite for the development of more effective therapeutic interventions. Transition from the inflammatory to the proliferative phase is a key step during healing and accumulating evidence associates a compromised transition with wound healing disorders. Thus, targeting factors that impact this phase transition may offer a rationale for therapeutic development. This review summarizes mechanisms regulating the inflammation–proliferation transition at cellular and molecular levels. We propose that identification of such mechanisms will reveal promising targets for development of more effective therapies.

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“…20 Diabetic wounds are often susceptible to hypoxia. For hypoxic cultures, 6 Â 10 5 cells were grown in complete MEM without FBS for 24 h. [21][22][23][24][25] Cultures were then incubated in an anaerobic jar with an anaerobic gas pack (Davies Diagnostics, Randburg, South Africa; Mitsubishi Gas Chemical Co., 4371051) for 4 h 26 to induce a hypoxic insult. Incubation with the gas pack results in 0% O 2 and 20% CO 2 within 2 h. 27…”

Section: Cell Culturementioning

confidence: 99%

Irradiation at 636 nm Positively Affects Diabetic Wounded and Hypoxic Cellsin Vitro

Sekhejane

Houreld²,

Abrahamse³

2011

Photomedicine and Laser Surgery

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Sphingosylphosphorylcholine-induced interleukin-6 production is mediated by protein kinase C and p42/44 extracellular signal-regulated kinase in human dermal fibroblasts

Cited by 14 publications

References 37 publications

Interleukin-6 Expression under Gravitational Stress Due to Vibration and Hypergravity in Follicular Thyroid Cancer Cells

Interleukin-6 Expression under Gravitational Stress Due to Vibration and Hypergravity in Follicular Thyroid Cancer Cells

Transition from inflammation to proliferation: a critical step during wound healing

Irradiation at 636 nm Positively Affects Diabetic Wounded and Hypoxic Cellsin Vitro

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