Sphingosine kinase AtSPHK1 functions in fumonisin B1-triggered cell death in Arabidopsis

Qin, Xiaoya; Zhang, Ruoxi; Ge, Shengchao; Zhou, Tao; Liang, You-Sheng

doi:10.1016/j.plaphy.2017.08.008

Cited by 31 publications

(24 citation statements)

References 60 publications

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“…Based on the SL profiling results after SL inhibitor treatment, GlcHCers were significantly suppressed in the PDMP-treated seedlings condition, however, DMS-treated seedlings firmly showed high GlcHCers in comparison with seedlings subjected to the mock condition. It has been reported that SPHK1 overexpression plant showed excess callose deposition at Arabidopsis rosette leaves, and it was restricted in the SPHK1-KD mutant plant upon fumonisin B1-triggered cell death condition (Qin et al, 2017). Therefore, we employed sphk1 ( sphk1-2 and SPHK1-KD ) ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The Arabidopsis thaliana ecotype Colombia-0 (Col-0) and the following mutants were used: SPHK1-KD (Sail_794_B01) (Worrall et al, 2008; Qin et al, 2017), sphk1-2 (Salk_042166), and gcs-2 (SK31705). The following constructs for the PD reporter were previously described: p35S::SP:GFP:PdBG2 (Grison et al, 2015) and (Simpson et al, 2009), p35S::SP:GFP:PDCB1 (Grison et al, 2015) and (Simpson et al, 2009), p35S::PDLP1:GFP (Caillaud et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

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Glucosylhydroxyceramides Modulate Secretion Machinery of a Subset of Plasmodesmata Proteins and a Change in the Callose Accumulation

Iswanto

Shon

et al. 2020

Preprint

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The plasma membranes encapsulated in the plasmodesmata (PDs) with symplasmic nano-channels contain abundant lipid rafts, which are enriched by sphingolipids and sterols. The attenuation of sterol compositions has demonstrated the role played by lipid raft integrity in the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins, particularly affecting in the callose enhancement. The presence of callose at PD is tightly attributed to the callose metabolic enzymes, callose synthases (CalSs) and β-1,3-glucanases (BGs) in regulating callose accumulation and callose degradation, respectively. Sphingolipids have been implicated in signaling and membrane protein trafficking, however the underlying processes linking sphingolipid compositions to the control of symplasmic apertures remain unknown. A wide variety of sphingolipids in plants prompts us to investigate which sphingolipid molecules are important in regulating symplasmic apertures. Here, we demonstrate that perturbations of sphingolipid metabolism by introducing several potential sphingolipid (SL) pathway inhibitors and genetically modifying SL contents from two independent SL pathway mutants are able to modulate callose deposition to control symplasmic connectivity. Our data from pharmacological and genetic approaches show that the alteration in glucosylhydroxyceramides (GlcHCers) particularly disturb the secretory machinery for GPI-anchored PdBG2 protein, resulting in an over accumulated callose. Moreover, our results reveal that SL-enriched lipid rafts link symplasmic channeling to PD callose homeostasis by controlling the targeting of GPI-anchored PdBG2. This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting to specific destination of GPI-anchored PD proteins incorporated with GlcHCers contents.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Glucosylhydroxyceramides Modulate Secretion Machinery of a Subset of Plasmodesmata Proteins and a Change in the Callose Accumulation

Iswanto

Shon

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…By using SPHK1 overexpressing plants, SA production was enhanced in response to FB1 treatment. Conversely SPHK1-KD plants displayed an increase in JA-related transcripts and metabolites (Qin et al, 2017). Therefore, it was suggested that the balance between LCBs and LCB-Ps modulated by the activity of SPHK1 acted as a signal upstream of the SA/JA signalling pathways during FB1-induced cell death (Qin et al, 2017).…”

Section: Interplay With Ja Signalling Pathwaymentioning

confidence: 99%

Sphingolipids: towards an integrated view of metabolism during the plant stress response

et al. 2019

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Plants exist in an environment of changing abiotic and biotic stresses. They have developed a complex set of strategies to respond to these stresses and over recent years it has become clear that sphingolipids are a key player in these responses. Sphingolipids are not universally present in all three domains of life. Many bacteria and archaea do not produce sphingolipids but they are ubiquitous in eukaryotes and have been intensively studied in yeast and mammals. During the last decade there has been a steadily increasing interest in plant sphingolipids. Plant sphingolipids exhibit structural differences when compared with their mammalian counterparts and it is now clear that they perform some unique functions. Sphingolipids are recognised as critical components of the plant plasma membrane and endomembrane system. Besides being important structural elements of plant membranes, their particular structure contributes to the fluidity and biophysical order. Sphingolipids are also involved in multiple cellular and regulatory processes including vesicle trafficking, plant development and defence. This review will focus on our current knowledge as to the function of sphingolipids during plant stress responses, not only as structural components of biological membranes, but also as signalling mediators.

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“…Evan's blue staining of the leaf was used to detect cell death according to the method suggested by Qin et al (2017). Briefly, banana leaves were stained with 0.2% (W/ V) Evans blue at 25°C for 8 h. The leaves were washed with water to remove excess stain and then bleached in warm methanol: acetic acid: glycerol (3:2:1) solution to remove the chlorophyll (Rangani et al 2016).…”

Section: Determination Of Cell Deathmentioning

confidence: 99%

Impact of sea-salt on morpho-physiological and biochemical responses in banana (Musa acuminata cv. Berangan)

Mazumdar

Lau

Singh

et al. 2019

Physiol Mol Biol Plants

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Banana is often grown in coastal-regions, and while known for its sensitivity towards seawater, little is documented on the effect of sea-salt on the growth, physiology and metal homeostasis. Here we report that banana plantlets exposed to sea-salt at extreme (average seawater concentration; 52.7 dS m-1), severe (28.5 dS m-1) or moderate (10.2 dS m-1) salinity levels had reduced root length (2.0-6.0-fold), plant height (1.2-1.6-fold), leaf number (2.0-2.3-fold) and leaf area (3.3-4.0-fold) compared to control plantlets. Degradation of pigments (total chlorophyll: 1.3-12.3-fold, chlorophyll a: 1.3-9.2-fold; chlorophyll b: 1.3-6.9-fold lower and carotenoids: 1.4-3.7fold lower) reflected vulnerability of photosystems to salt stress. Relative water content showed a maximum decrease of 1.5-fold in salt stress. MDA analysis showed sea-salt exposure triggers 2.3-3.5-fold higher lipid peroxidation. Metal content analysis showed a 73-fold higher Na value from roots exposed to extreme salinity compared to control plantlets. While phenotype was clearly affected, moderate salinity showed no significant alteration of macro (N, P, K and Ca) and micro (Fe, Mn and Cu) metal content. The antioxidant enzymes: SOD (3.2-fold), CAT (1.7-fold) and GR (6-fold) showed higher activity at moderate salinity level compared to control plantlets but lower activity at severe (SOD: 1.3-fold; CAT: 1.5-fold; GR: 2-fold lower) and extreme seawater salinity (SOD: 1.5; CAT: 1.9; GR: 1.3-fold lower). Mild changes in growth and physiology at sea-salt levels equivalent to moderate seawater flooding, indicate that banana will survive such flooding, while extreme seawater inundation will be lethal. This data provides a reference for future salinity-mediated work in banana.

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Sphingosine kinase AtSPHK1 functions in fumonisin B1-triggered cell death in Arabidopsis

Cited by 31 publications

References 60 publications

Glucosylhydroxyceramides Modulate Secretion Machinery of a Subset of Plasmodesmata Proteins and a Change in the Callose Accumulation

Glucosylhydroxyceramides Modulate Secretion Machinery of a Subset of Plasmodesmata Proteins and a Change in the Callose Accumulation

Sphingolipids: towards an integrated view of metabolism during the plant stress response

Impact of sea-salt on morpho-physiological and biochemical responses in banana (Musa acuminata cv. Berangan)

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