Sphingosine kinase 1 expressed by endothelial colony-forming cells has a critical role in their revascularization activity

Poitevin, Stéphane; Cussac, Daniel; Leroyer, Aurélie S.; Albinet, Virginie; Sarlon-Bartoli, G.; Guillet, Benjamin; Hubert, Lucas; Andrieu‐Abadie, Nathalie; Couderc, Bettina; Parini, Angelo; Dignat-George, Françoise; Sabatier, Florence

doi:10.1093/cvr/cvu104

Cited by 35 publications

(42 citation statements)

References 42 publications

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“…6D). Furthermore, the expression of Bace2 and ApoE, both of which have been verified by us and by other researchers to be E-box genes that contain non-canonical E-box motifs in their promoter regions61, was also altered in the hippocampus of aged APP/PS1dE9 mice at ZT5, ZT11, and ZT17 (Fig. 6C).…”

Section: Resultssupporting

confidence: 78%

Orexin signaling regulates both the hippocampal clock and the circadian oscillation of Alzheimer’s disease-risk genes

Jiang

Zhang

2016

Sci Rep

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Alzheimer’s disease (AD) is a circadian clock-related disease. However, it is not very clear whether pre-symptomatic AD leads to circadian disruption or whether malfunction of circadian rhythms exerts influence on development of AD. Here, we report a functional clock that exists in the hippocampus. This oscillator both receives input signals and maintains the cycling of the hippocampal Per2 gene. One of the potential inputs to the oscillator is orexin signaling, which can shorten the hippocampal clock period and thereby regulate the expression of clock-controlled-genes (CCGs). A 24-h time course qPCR analysis followed by a JTK_CYCLE algorithm analysis indicated that a number of AD-risk genes are potential CCGs in the hippocampus. Specifically, we found that Bace1 and Bace2, which are related to the production of the amyloid-beta peptide, are CCGs. BACE1 is inhibited by E4BP4, a repressor of D-box genes, while BACE2 is activated by CLOCK:BMAL1. Finally, we observed alterations in the rhythmic expression patterns of Bace2 and ApoE in the hippocampus of aged APP/PS1dE9 mice. Our results therefore indicate that there is a circadian oscillator in the hippocampus whose oscillation could be regulated by orexins. Hence, orexin signaling regulates both the hippocampal clock and the circadian oscillation of AD-risk genes.

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Section: Resultssupporting

confidence: 78%

Orexin signaling regulates both the hippocampal clock and the circadian oscillation of Alzheimer’s disease-risk genes

Jiang

Zhang

2016

Sci Rep

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“…8 S1P promotes initial sprouting of capillary-like structures by endothelial cells in vitro 13 and synergistically acts with basic fibroblast growth factor for induction of in vitro angiogenesis. 63 In addition, S1P plays a crucial role in stabilizing neovessels in arteriogenesis via the recruitment of mural cells and regulation of endothelial cell-cell junctions.…”

Section: Figmentioning

confidence: 99%

“…While these lipids can have similar effects on cellular response, they possess distinct chemical backbones, mechanisms of synthesis and degradation, and signaling pathways mediated by a discrete set of G-proteincoupled receptors (GPCRs). LPA and S1P predominantly act extracellularly on their GPCRs and can directly regulate cell survival, 2,4,5 differentiation, 2,6-8 cytokine secretion, 9,10 proliferation, [11][12][13] and migration 2,12,13 -each of the important functions that must be considered in regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

Lysophosphatidic Acid and Sphingosine-1-Phosphate: A Concise Review of Biological Function and Applications for Tissue Engineering

Binder

Williams

Silva

et al. 2015

Tissue Engineering Part B: Reviews

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The presentation and controlled release of bioactive signals to direct cellular growth and differentiation represents a widely used strategy in tissue engineering. Historically, work in this field has primarily focused on the delivery of large cytokines and growth factors, which can be costly to manufacture and difficult to deliver in a sustained manner. There has been a marked increase over the past decade in the pursuit of lipid mediators due to their wide range of effects over multiple cell types, low cost, and ease of scale-up. Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are two bioactive lysophospholipids (LPLs) that have gained attention for use as pharmacological agents in tissue engineering applications. While these lipids can have similar effects on cellular response, they possess distinct chemical backbones, mechanisms of synthesis and degradation, and signaling pathways using a discrete set of G-protein-coupled receptors (GPCRs). LPA and S1P predominantly act extracellularly on their GPCRs and can directly regulate cell survival, differentiation, cytokine secretion, proliferation, and migration-each of the important functions that must be considered in regenerative medicine. In addition to these potent physiological functions, these LPLs play pivotal roles in a number of pathophysiological processes. To capitalize on the promise of these molecules in tissue engineering, these lipids have been incorporated into biomaterials for in vivo delivery. Here, we survey the effects of LPA and S1P on both cellular-and tissue-level phenotypes, with an eye toward regulating stem/progenitor cell growth and differentiation. In particular, we examine work that has translational applications for cell-based tissue engineering strategies in promoting cell survival, bone and cartilage engineering, and therapeutic angiogenesis.

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“…Here, we demonstrated that both SK1 and SK2 expressed by H-MVEC have a critical role in capillary morphogenesis of MAB cultured together with H-MVEC in Matrigel. Intriguingly, a recent report demonstrated that the up-regulation of SK1 in endothelial colony-forming cells induced by bone marrow mesenchymal stem cells is critical for their angiogenic/vasculogenic activity [35], supporting the view that a mutual communication between endothelial and mesenchymal cells takes place promoting the formation and the stabilization of new vessels. Although SK1 and SK2 have been often reported to have distinct biological functions based on their different localization inside the cell and tissue distribution [4], in this study, both isoforms were found to be involved, although at a different extent, in the observed biological processes.…”

Section: Discussionmentioning

confidence: 74%

Endothelial sphingosine kinase/SPNS2 axis is critical for vessel-like formation by human mesoangioblasts

et al. 2015

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• Down-regulation of SK1/2 in H-MVEC impaired vessel formation when cultured with MAB. • H-MVEC SPNS2 is critical for morphogenesis and migration induced by H-MVEC CM of MAB. • CM from SK1- and SK2-siRNA H-MVEC impaired morphogenesis and migration of MAB. • S1P1/3 were involved on CM-induced morphogenesis and migration of MAB. • Matrigel plug assay showed the role of S1P axis in MAB-endothelial cell interaction.

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Sphingosine kinase 1 expressed by endothelial colony-forming cells has a critical role in their revascularization activity

Abstract: The up-regulation of SphK1 and S1P-dependent pathways is critical for the angiogenic/vasculogenic activity of ECFCs. The identification of this pathway provides attractive targets to optimize cell-based therapy for revascularization in ischaemic diseases.

Cited by 35 publications

References 42 publications

Orexin signaling regulates both the hippocampal clock and the circadian oscillation of Alzheimer’s disease-risk genes

Orexin signaling regulates both the hippocampal clock and the circadian oscillation of Alzheimer’s disease-risk genes

Lysophosphatidic Acid and Sphingosine-1-Phosphate: A Concise Review of Biological Function and Applications for Tissue Engineering

Endothelial sphingosine kinase/SPNS2 axis is critical for vessel-like formation by human mesoangioblasts

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