Sphingosine-1-Phosphate-Receptor 1 as a Marker for Endothelial Cells in Mouse Xenograft Models of Human Cancer

Lüders, Nadine; Schumacher, Udo

doi:10.21873/anticanres.11731

Cited by 2 publications

(1 citation statement)

References 17 publications

(19 reference statements)

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“…FFPE sections of human ovarian cancer cell lines OVCAR3 and SKOV3 grown as intraperitoneal tumours in SCID mice from previously published experiments were drawn from the files of the Institute of Anatomy and Experimental Morphology and processed in the same way as the clinical samples [ 19 , 21 – 23 ]. In addition, in vitro grown ovarian cancer cells were fixed in formalin and embedded in agar in previous experiment of out institute (for cell lines used see section below) [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Ovarian cancer cells regulate their mitochondrial content and high mitochondrial content is associated with a poor prognosis

Weigelt,

Petrosyan,

Oliveira-Ferrer

et al. 2024

BMC Cancer

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Most cancer patients ultimately die from the consequences of distant metastases. As metastasis formation consumes energy mitochondria play an important role during this process as they are the most important cellular organelle to synthesise the energy rich substrate ATP, which provides the necessary energy to enable distant metastasis formation. However, mitochondria are also important for the execution of apoptosis, a process which limits metastasis formation. We therefore wanted to investigate the mitochondrial content in ovarian cancer cells and link its presence to the patient’s prognosis in order to analyse which of the two opposing functions of mitochondria dominates during the malignant progression of ovarian cancer. Monoclonal antibodies directed against different mitochondrial specific proteins, namely heat shock proteins 60 (HSP60), fumarase and succinic dehydrogenase, were used in immunohistochemistry in preliminary experiments to identify the antibody most suited to detect mitochondria in ovarian cancer cells in clinical tissue samples. The clearest staining pattern, which even delineated individual mitochondria, was seen with the anti-HSP60 antibody, which was used for the subsequent clinical study staining primary ovarian cancers (n = 155), borderline tumours (n = 24) and recurrent ovarian cancers (n = 26). The staining results were semi-quantitatively scored into three groups according to their mitochondrial content: low (n = 26), intermediate (n = 50) and high (n = 84). Survival analysis showed that high mitochondrial content correlated with a statistically significant overall reduced survival rate In addition to the clinical tissue samples, mitochondrial content was analysed in ovarian cancer cells grown in vitro (cell lines: OVCAR8, SKOV3, OVCAR3 and COV644) and in vivo in severe combined immunodeficiency (SCID) mice.In in vivo grown SKOV3 and OVCAR8 cells, the number of mitochondria positive cells was markedly down-regulated compared to the in vitro grown cells indicating that mitochondrial number is subject to regulatory processes. As high mitochondrial content is associated with a poor prognosis, the provision of high energy substrates by the mitochondria seems to be more important for metastasis formation than the inhibition of apoptotic cell death, which is also mediated by mitochondria. In vivo and in vitro grown human ovarian cancer cells showed that the mitochondrial content is highly adaptable to the growth condition of the cancer cells.

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Section: Methodsmentioning

confidence: 99%

Ovarian cancer cells regulate their mitochondrial content and high mitochondrial content is associated with a poor prognosis

Weigelt,

Petrosyan,

Oliveira-Ferrer

et al. 2024

BMC Cancer

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Locally Ablative Radiation Therapy of a Primary Human Small Cell Lung Cancer Tumor Decreases the Number of Spontaneous Metastases in Two Xenograft Models

Frenzel

Siekmann

Grohmann

et al. 2018

International Journal of Radiation Oncology*Biology*Physics

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Sphingosine-1-Phosphate-Receptor 1 as a Marker for Endothelial Cells in Mouse Xenograft Models of Human Cancer

Abstract: As S1PR is expressed in most blood vessel endothelia and in the tumor blood vessel endothelia, it is an ideal blood vessel marker.

Cited by 2 publications

References 17 publications

Ovarian cancer cells regulate their mitochondrial content and high mitochondrial content is associated with a poor prognosis

Ovarian cancer cells regulate their mitochondrial content and high mitochondrial content is associated with a poor prognosis

Locally Ablative Radiation Therapy of a Primary Human Small Cell Lung Cancer Tumor Decreases the Number of Spontaneous Metastases in Two Xenograft Models

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