Sphingomyelinase Treatment Induces ATP-independent Endocytosis

Zha, Xiaohui; Pierini, Lynda M.; Leopold, Philip L.; Skiba, Paul J.; Tabas, Ira; Maxfield, Frederick R.

doi:10.1083/jcb.140.1.39

Cited by 192 publications

(161 citation statements)

References 46 publications

Supporting

Mentioning

147

Contrasting

Order By: Relevance

“…Two groups have reported that ASMase signaling may occur in caveolae, which may represent transient membrane structures capable of forming as a physical response of membranes to diverse stresses (Dobrowsky and Carter, 1998;Liu and Anderson, 1995). Consistent with this hypothesis recent studies show that ceramide generation (Zha et al, 1998) via ASMase (Grassme et al, 1997) is capable of initiating formation of a vesicular compartment at the plasma membrane in an ATP-independent manner probably due in part to the unique property of ceramide to induce non-lamellar structures in bilayers (RuizArguello et al, 1998).…”

Section: Mechanisms Of Ceramide Generationmentioning

confidence: 70%

Stress signals for apoptosis: ceramide and c-Jun kinase

1998

View full text Add to dashboard Cite

Section: Mechanisms Of Ceramide Generationmentioning

confidence: 70%

Stress signals for apoptosis: ceramide and c-Jun kinase

1998

View full text Add to dashboard Cite

“…Treatment of J774 macrophages and Chinese hamster ovary cells with exogenous SMase has been found to induce rapid formation of endocytic vesicles (65). The SMase-induced vesicles, which are ϳ400 nm in diameter and not enriched in clathrin or caveolin, pinch off from the plasma membrane and move into the cytosol (65).…”

Section: Discussionmentioning

confidence: 99%

Ceramide in Lipid Particles Enhances Heparan Sulfate Proteoglycan and Low Density Lipoprotein Receptor-related Protein-mediated Uptake by Macrophages

Morita

Kawabe

Sakurai

et al. 2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

Arterial wall sphingomyelinase (SMase) has been proposed to be involved in atherogenesis. SMase modification of lipoproteins has been shown to occur in atherosclerotic lesions and to facilitate their uptake by macrophages and foam cell formation. To investigate the mechanism of macrophage uptake enhanced by SMase, we prepared lipid emulsions containing sphingomyelin (SM) or ceramide (CER) as model particles of lipoproteins. SMase remarkably increased the uptake of SM-containing emulsions by J774 macrophages without apolipoproteins. The emulsion uptake was negatively correlated with the degree of particle aggregation by pretreatment with SMase, whereas the uptake of CERcontaining emulsions was significantly larger than SMcontaining emulsions, indicating that enhancement of uptake is due to the generation of CER molecules in particles but not to the aggregation by SMase. Heparan sulfate proteoglycans (HSPGs) and low density lipoprotein receptor-related protein (LRP) were crucial for CER-enhanced emulsion uptake, because heparin or lactoferrin inhibited the emulsion uptake. Confocal microscopy also showed that SMase promoted both binding and internalization of emulsions by J774 macrophages, which were almost abolished by lactoferrin. Apolipoprotein E further increased the uptake of CERcontaining emulsions compared with SM-containing emulsions. These findings suggest the generation of CER in lipoproteins by SMase facilitates the macrophage uptake via HSPG and LRP pathways and plays a crucial role in foam cell formation. Thus, CER may act as an important atherogenic molecule.

show abstract

“…For example, the production of ceramide by sphingomyelinase elicits phagocytosis (Grassme et al, 1997;Hinkovska-Galcheva et al, 1998;Zha et al, 1998). Likewise, ceramide treatment has been shown to induce the formation of endocytic vesicles (Li et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

t-SNARE Phosphorylation Regulates Endocytosis in Yeast

Gurunathan

Marash

Weinberger

et al. 2002

MBoC

View full text Add to dashboard Cite

Earlier we demonstrated that activation of a ceramide-activated protein phosphatase (CAPP) conferred normal growth and secretion to yeast lacking their complement of exocytic v-SNAREs (Snc1,2) or bearing a temperature-sensitive mutation in an exocytic t-SNARE (Sso2). CAPP activation led to Sso dephosphorylation and enhanced the assembly of t-SNAREs into functional complexes. Thus, exocytosis in yeast is modulated by t-SNARE phosphorylation. Here, we show that endocytic defects in cells lacking the v-and t-SNAREs involved in endocytosis are also rescued by CAPP activation. Yeast lacking the Tlg1 or Tlg2 t-SNAREs, the Snc v-SNAREs, or both, undergo endocytosis after phosphatase activation. CAPP activation correlated with restored uptake of FM4-64 to the vacuole, the uptake and degradation of the Ste2 receptor after mating factor treatment, and the dephosphorylation and assembly of Tlg1,2 into SNARE complexes. Activation of the phosphatase by treatment with C 2 -ceramide, VBM/ELO gene inactivation, or by the overexpression of SIT4 was sufficient to confer rescue. Finally, we found that mutation of single PKA sites in Tlg1 (Ser31 to Ala31) or Tlg2 (Ser90 to Ala90) was sufficient to restore endocytosis, but not exocytosis, to snc cells. These results suggest that endocytosis is also modulated by t-SNARE phosphorylation in vivo.

show abstract

Sphingomyelinase Treatment Induces ATP-independent Endocytosis

Cited by 192 publications

References 46 publications

Stress signals for apoptosis: ceramide and c-Jun kinase

Stress signals for apoptosis: ceramide and c-Jun kinase

Ceramide in Lipid Particles Enhances Heparan Sulfate Proteoglycan and Low Density Lipoprotein Receptor-related Protein-mediated Uptake by Macrophages

t-SNARE Phosphorylation Regulates Endocytosis in Yeast

Contact Info

Product

Resources

About