Sphingomyelin synthesis is involved in adherence during macrophage differentiation of HL-60 cells

Dressler, Kenneth A.; Kan, Chu-Cheng; Kolesnick, Richard

doi:10.1016/s0021-9258(18)98988-x

Cited by 29 publications

(5 citation statements)

References 29 publications

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“…[ 3 H]Sph was also converted by N-acylation into Cer (Goldkorn et al, 1991) at later time points (Figure 1A); 3%, 7%, and 10% of added [ 3 H]Sph was converted into Cer at 1, 2, and 3 h, respectively. Cer can be utilized in the synthesis of complex sphingolipids such as sphingomyelin and glycosphingolipids (Dressler et al, 1991). However, the incorporation of [ 3 H]-Sph into sphingomyelin was very low with a lag period of 2 h, and no radioactive glycosphingolipids were detected in platelets under our experimental conditions (Figure 1A).…”

Section: Metabolic Fates Of [ 3 H]sph [ 14 C]dms [ 14 C]tms and [ 3 H...mentioning

confidence: 78%

N,N-Dimethylsphingosine Inhibition of Sphingosine Kinase and Sphingosine 1-Phosphate Activity in Human Platelets

et al. 1996

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Potential sphingosine (Sph) metabolites include phosphorylated, N-acylated, and N-methylated derivatives. Phosphorylated Sph, i.e., sphingosine 1-phosphate (Sph-1-P), may act as an autocrine stimulator of blood platelets, as it is abundantly stored in platelets and released extracellularly and its exogenous addition induces platelet activation. In this study, we evaluated Sph-1-P formation and its effects in human platelets in the presence of other Sph metabolites. On addition of [3H]Sph to intact platelets, the label was rapidly converted to Sph-1-P. This conversion into [3H]Sph-1-P was inhibited by N,N-dimethylsphingosine (DMS) in a dose-dependent manner, but not by other structurally related Sph derivatives, including ceramide. The inhibition of Sph-1-P formation by DMS was reproduced using a cell-free system (Sph kinase obtained from platelet cytosolic fractions) and much stronger than that by DL-threo-dihydrosphingosine, which had been considered to be the strongest inhibitor of Sph kinase. Administration of DMS to intact platelets resulted in a decrease in Sph-1-P mass and an increase in Sph mass. Furthermore, DMS inhibited the release of Sph-1-P from platelets stimulated with 12-O-tetradecanoylphorbol 13-acetate and inhibited platelet aggregation induced by exogenous addition of Sph-1-P. Collectively, our results indicate that DMS is useful as a Sph kinase inhibitor and that Sph-1-P actions as an autocrine stimulator of platelets are inhibited by DMS.

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Section: Metabolic Fates Of [ 3 H]sph [ 14 C]dms [ 14 C]tms and [ 3 H...mentioning

confidence: 78%

N,N-Dimethylsphingosine Inhibition of Sphingosine Kinase and Sphingosine 1-Phosphate Activity in Human Platelets

et al. 1996

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“…In some cell models, such as U937 and HL-60 treated with TPA, stimulation of the initial steps of the synthesis of glycosphingolipids was also observed with the increased activity of GCS and accumulation of glucosylceramide [144,145]. Similarly, an increase of SM synthesis also accompanied monocytic/macrophage differentiation and these complex sphingolipid changes were reflective of only the adherent portion of the differentiating cells [145,146]. Importantly, both the accumulation of glycosphingolipids and SM seems to contribute to adhesion of macrophage-like cells, as treatment with PDMP, an inhibitor of GCS, or exogenously added bacterial sphingomyelinase blocked adherence of the differentiated cells.…”

Section: Sphingolipids In Myeloid Differentiationmentioning

confidence: 98%

Sphingolipids in Hematopoiesis: Exploring Their Role in Lineage Commitment

Raza

Salman

Luberto

2021

Cells

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Sphingolipids, associated enzymes, and the sphingolipid pathway are implicated in complex, multifaceted roles impacting several cell functions, such as cellular homeostasis, apoptosis, cell differentiation, and more through intrinsic and autocrine/paracrine mechanisms. Given this broad range of functions, it comes as no surprise that a large body of evidence points to important functions of sphingolipids in hematopoiesis. As the understanding of the processes that regulate hematopoiesis and of the specific characteristics that define each type of hematopoietic cells is being continuously refined, the understanding of the roles of sphingolipid metabolism in hematopoietic lineage commitment is also evolving. Recent findings indicate that sphingolipid alterations can modulate lineage commitment from stem cells all the way to megakaryocytic, erythroid, myeloid, and lymphoid cells. For instance, recent evidence points to the ability of de novo sphingolipids to regulate the stemness of hematopoietic stem cells while a substantial body of literature implicates various sphingolipids in specialized terminal differentiation, such as thrombopoiesis. This review provides a comprehensive discussion focused on the mechanisms that link sphingolipids to the commitment of hematopoietic cells to the different lineages, also highlighting yet to be resolved questions.

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“…The mass amounts of ceramide in cellular extracts were measured by the use of the DAG kinase enzymatic method (Schneider and Kennedy, 1973;Dressier and Kolesnick, 1991). Cells were treated with SPC or vehicle for various incubation periods.…”

Section: Mass Measurement Of Ceramide and Sphingosinementioning

confidence: 99%

“…Brady et al first reported the formation of [t4C]sphingomyelin after incubation of SPC and stearoyl-[t4C]CoA with rat brain homogenates (Brady et al, 1965). SPC was recently reported to stimulate an increase in sphingomyelin levels in neuroblastoma cells (Sugiyama et al, 1990) and in human leukemia HL-60 cells (Dressier et al, 1991). In both of these studies, the increase in levels of sphingomyelin was associated with differentiation.…”

Section: Is Endogenous Spc a Regulator Of Mitogenesis?mentioning

confidence: 99%

Signaling pathways for sphingosylphosphorylcholine-mediated mitogenesis in Swiss 3T3 fibroblasts.

Desai

Carlson

Mattie

et al. 1993

The Journal of Cell Biology

118

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Abstract. Sphingosylphosphorylcholine (SPC), or lysophingomyelin, a wide-spectrum growth promoting agent for a variety of cell types (Desai, N. N., and S. Spiegel. 1991. Biochem. Biophys. Res. Comm. 181: 361-366), stimulates cellular proliferation of quiescent Swiss 3T3 fibroblasts to a greater extent than other known growth factors or than the structurally related molecules, sphingosine and sphingosine-l-phosphate.

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Sphingomyelin synthesis is involved in adherence during macrophage differentiation of HL-60 cells

Cited by 29 publications

References 29 publications

N,N-Dimethylsphingosine Inhibition of Sphingosine Kinase and Sphingosine 1-Phosphate Activity in Human Platelets

N,N-Dimethylsphingosine Inhibition of Sphingosine Kinase and Sphingosine 1-Phosphate Activity in Human Platelets

Sphingolipids in Hematopoiesis: Exploring Their Role in Lineage Commitment

Signaling pathways for sphingosylphosphorylcholine-mediated mitogenesis in Swiss 3T3 fibroblasts.

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