Sphingolipid synthesis inhibitor fumonisin B1 causes verticillium wilt in cotton

Xu, Fan; Huang, Li; Wang, Junyao; Ma, Cun‐Gen; Tan, Yinqing; Wang, Fanlong; Fan, Yanhua; Luo, Ming

doi:10.1111/jipb.13241

Cited by 10 publications

(4 citation statements)

References 32 publications

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“…HPLC assay showed that the peak profile of the Δ VdDrs2 fermentation broth was different with the wild type: both the peaks height and peaks area of the Δ VdDrs2 fermentation broth were lower than those of the wild type, showing that a decrease in metabolites secreted by knockout strain ( Figures 6A,B ). As there are two toxins, SFA and FB1, identified in V. dahliae ( Zhang et al, 2016 ; Xu et al, 2022 ), we quantified the amount of these two toxins, in the fermentation broths of Vd991 and Δ VdDrs2 . The amounts of these two VW toxins produced by one mg of Δ VdDrs2 mycelia were significantly lower than that by wild type ( Figure 6C ).…”

Section: Resultsmentioning

confidence: 99%

Loss of function of VdDrs2, a P4-ATPase, impairs the toxin secretion and microsclerotia formation, and decreases the pathogenicity of Verticillium dahliae

Ren

Li²,

Li³

et al. 2022

Front. Plant Sci.

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Four P4-ATPase flippase genes, VdDrs2, VdNeo1, VdP4-4, and VdDnf1 were identified in Verticillium dahliae, one of the most devastating phytopathogenic fungi in the world. Knock out of VdDrs2, VdNeo1, and VdP4-4, or knock down of VdDnf1 significantly decreased the pathogenicity of the mutants in cotton. Among the mutants, the greatest decrease in pathogenicity was observed in ΔVdDrs2. VdDrs2 was localized to plasma membrane, vacuoles, and trans-Golgi network (TGN). In vivo observation showed that the infection of the cotton by ΔVdDrs2 was significantly delayed. The amount of two known Verticillium toxins, sulfacetamide, and fumonisin B1 in the fermentation broth produced by the ΔVdDrs2 strain was significantly reduced, and the toxicity of the crude Verticillium wilt toxins to cotton cells was attenuated. In addition, the defect of VdDrs2 impaired the synthesis of melanin and the formation of microsclerotia, and decreased the sporulation of V. dahliae. Our data indicate a key role of P4 ATPases-associated vesicle transport in toxin secretion of disease fungi and support the importance of mycotoxins in the pathogenicity of V. dahliae.

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Section: Resultsmentioning

confidence: 99%

Loss of function of VdDrs2, a P4-ATPase, impairs the toxin secretion and microsclerotia formation, and decreases the pathogenicity of Verticillium dahliae

Ren

Li²,

Li³

et al. 2022

Front. Plant Sci.

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“…Sphingolipids were extracted from the ten seeds after 0 h, 6 h, 12 h, and 24 h imbibition with three biological repeats and detected by LipidALL Technologies Co. Ltd. (http://www.lipidall.com/) as described by Xu et al [51]. Brie y, collected soybean seeds were weighed and inactivated with hot isopropanol using a modi ed protocol as described [52].…”

Section: Extraction and Detection Of Sphingolipidsmentioning

confidence: 99%

Integrated time-course transcriptome and sphingolipidomics revealed that saturated ceramide accelerated seed germination in soybean

Liu,

Zhang,

Qiao

et al. 2024

Preprint

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Background Soybean (Glycine max) is one of the most important oil and fodder crop worldwide. High and uniform seed germination is crucial for soybean yield. Sphingolipid is one of the major components of membrane lipid raft, which has crucial roles in plant growth and stress responses. However, Knowledge regarding the functions of sphingolipid in seed germination is elusive. Results Through chemicobiological methods, we found that exogenous application of C24 phytoceramide t18:0/24:0 could accelerate seed germination in soybean. In order to explore the functions of sphingolipid in soybean seed germination, we performed integrated time-course transcriptome and sphingolipidomics with germinated soybean seeds. We identified two modules of gene set that were positively or negatively associated with seed germination by time-course transcriptome analysis, and the expression of these genes were gradually up- or down- regulated during seed germination, respectively. Among which, many genes involved in sphingolipid biosynthesis were down-regulated during seed germination, especially, the LCB Δ8 desaturases attracted our attentions. Moreover, our time-course sphingolipidomics results indicated that the amounts of ceramide d18:0/16:0, ceramide d18:0/22:0, and phytoceramide t18:0/24:0 were elevated during seed germination, suggesting that saturated ceramides is positive related to seed germination in soybean. Conclusion Through time-course transcriptome analysis, we identified two set genes that related to soybean seed germination. Moreover, consider with time-course sphingolipidomics and chemicobiological assays, we demonstrated that saturated simple ceramides have positive roles in soybean seed germination.

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“…For F. proliferatum , which causes rice spikelet rot disease, it was shown that the disruption of several genes leading to loss of fumonisin production caused reduced virulence [ 23 ]. Also, in Verticillium dahliae causing wilting disease in cotton, fumonisin-deficient knockout strains were less virulent [ 24 ]. In the case of Alternaria alternata f.sp.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Fumonisin Self-Resistance: Fusarium verticillioides Contains Four Fumonisin B1-Insensitive-Ceramide Synthases

Krska,

Twaruschek,

Wiesenberger

et al. 2024

Toxins

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Fusarium verticillioides produces fumonisins, which are mycotoxins inhibiting sphingolipid biosynthesis in humans, animals, and other eukaryotes. Fumonisins are presumed virulence factors of plant pathogens, but may also play a role in interactions between competing fungi. We observed higher resistance to added fumonisin B1 (FB1) in fumonisin-producing Fusarium verticillioides than in nonproducing F. graminearum, and likewise between isolates of Aspergillus and Alternaria differing in production of sphinganine-analog toxins. It has been reported that in F. verticillioides, ceramide synthase encoded in the fumonisin biosynthetic gene cluster is responsible for self-resistance. We reinvestigated the role of FUM17 and FUM18 by generating a double mutant strain in a fum1 background. Nearly unchanged resistance to added FB1 was observed compared to the parental fum1 strain. A recently developed fumonisin-sensitive baker’s yeast strain allowed for the testing of candidate ceramide synthases by heterologous expression. The overexpression of the yeast LAC1 gene, but not LAG1, increased fumonisin resistance. High-level resistance was conferred by FUM18, but not by FUM17. Likewise, strong resistance to FB1 was caused by overexpression of the presumed F. verticillioides “housekeeping” ceramide synthases CER1, CER2, and CER3, located outside the fumonisin cluster, indicating that F. verticillioides possesses a redundant set of insensitive targets as a self-resistance mechanism.

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Sphingolipid synthesis inhibitor fumonisin B1 causes verticillium wilt in cotton

Cited by 10 publications

References 32 publications

Loss of function of VdDrs2, a P4-ATPase, impairs the toxin secretion and microsclerotia formation, and decreases the pathogenicity of Verticillium dahliae

Loss of function of VdDrs2, a P4-ATPase, impairs the toxin secretion and microsclerotia formation, and decreases the pathogenicity of Verticillium dahliae

Integrated time-course transcriptome and sphingolipidomics revealed that saturated ceramide accelerated seed germination in soybean

Mechanism of Fumonisin Self-Resistance: Fusarium verticillioides Contains Four Fumonisin B1-Insensitive-Ceramide Synthases

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