Sphingolipid long chain bases

Karlsson, Karl‐Anders

doi:10.1007/bf02531119

Cited by 239 publications

(138 citation statements)

References 112 publications

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“…For PE-pl, mass spectrometric analysis allows to differentiate ether and fatty acid linked to the glycerol backbone. SM species assignment includes sphingoid base and N-linked fatty acid, because plasma SM contains beside sphingosine d18:1 (62%), other sphingoid bases, mainly sphingadienine d18:2 (14%) and hexadecasphingosine d16:1 (10%), in considerable amounts (33). Lysophospholipids (LPC), CER, and CE contain one fatty acid denominated by the species nomenclature.…”

Section: Lipid Species Pattern Of Lipoprotein Classesmentioning

confidence: 99%

Lipid profiling of FPLC-separated lipoprotein fractions by electrospray ionization tandem mass spectrometry

Wiesner

Leidl

Boettcher

et al. 2009

Journal of Lipid Research

312

306

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Glycerophospholipid and sphingolipid species and their bioactive metabolites are important regulators of lipoprotein and cell function. The aim of the study was to develop a method for lipid species profiling of separated lipoprotein classes. Human serum lipoproteins VLDL, LDL, and HDL of 21 healthy fasting blood donors were separated by fast performance liquid chromatography (FPLC) from 50 ml serum. Subsequently, phosphatidylcholine (PC), lysophosphatidylcholine, sphingomyelin (SM), ceramide (CER), phosphatidylethanolamine (PE), PE-based plasmalogen (PE-pl), cholesterol, and cholesteryl ester (CE) content of the separated lipoproteins was quantified by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Analysis of FPLC fractions with PAGE demonstrated that albumin partially coelutes with HDL fractions. However, analysis of an HDL deficient serum (Tangier disease) showed that only lysophosphatidylcholine, but none of the other lipids analyzed, exhibited a significant coelution with the albumin containing fractions. Approximately 60% of lipoprotein CER were found in LDL fractions and 60% of PC, PE, and plasmalogens in HDL fractions. VLDL, LDL, and HDL displayed characteristic lipid class and species pattern. The developed method provides a detailed lipid class and species composition of lipoprotein fractions and may serve as a valuable tool to identify alterations of lipoprotein lipid species profiles in disease with a reasonable experimental effort.-Wiesner, P., K. Leidl, A. Boettcher, G. Schmitz, and G. Liebisch. Lipid profiling of FPLC-separated lipoprotein fractions by electrospray ionization tandem mass spectrometry.

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Section: Lipid Species Pattern Of Lipoprotein Classesmentioning

confidence: 99%

Lipid profiling of FPLC-separated lipoprotein fractions by electrospray ionization tandem mass spectrometry

Wiesner

Leidl

Boettcher

et al. 2009

Journal of Lipid Research

312

306

View full text Add to dashboard Cite

show abstract

“…They encompass a wide array of 2-amino-1,3-dihydroxyalkanes or -enes with (2S,3R)-erythro stereochemistry, alkyl chain lengths from 14 to 22 carbon atoms, 0 to 2 double bonds, and other modifications, such as hydroxyl group(s) at positions 4 or 6 and branching methyl groups at -l (iso), -2 (anti-iso), or elsewhere (6,7). Mammals produce mainly the species shown in Fig.…”

Section: Structural Diversity Of Sphingoid Basesmentioning

confidence: 99%

De Novo Sphingolipid Biosynthesis: A Necessary, but Dangerous, Pathway

Merrill

2002

Journal of Biological Chemistry

548

231

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“…It is well known that there are diverse structures of sphingoid bases in nature 15,16 . The most common sphingoid base of mammalian sphingolipids is sphingosine trans-4-sphingenine, d18:1 4t .…”

Section: Introductionmentioning

confidence: 99%

Analysis of Glucosylceramides from Various Sources by Liquid Chromatography-Ion Trap Mass Spectrometry

et al. 2010

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Sphingolipid long chain bases

Abstract: About 60 natural sphingolipid long chain bases have been identified or proposed. A review is given on their nomenclature, distribution, metabolism, biologic properties, chemistry and methods of characterization.

Cited by 239 publications

References 112 publications

Lipid profiling of FPLC-separated lipoprotein fractions by electrospray ionization tandem mass spectrometry

Lipid profiling of FPLC-separated lipoprotein fractions by electrospray ionization tandem mass spectrometry

De Novo Sphingolipid Biosynthesis: A Necessary, but Dangerous, Pathway

Analysis of Glucosylceramides from Various Sources by Liquid Chromatography-Ion Trap Mass Spectrometry

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