Sphingobium aquiterrae sp. nov., a toluene, meta- and para-xylene-degrading bacterium isolated from petroleum hydrocarbon-contaminated groundwater

Révész, Fruzsina; Tóth, Erika; Kriszt, Balázs; Bóka, Károly; Benedek, Tibor; Sárkány, Orsolya; Nagy, Z.; Táncsics, Andräs

doi:10.1099/ijsem.0.002898

Cited by 24 publications

(10 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the most abundant genus in the sample LDS, Simplicispira likely involve nitrate reduction as the strains of the genus Simplicispira isolated from freshwater, soil, and activated sludge were reported to be capable of denitrification 63 . Many members of Pseudomonas and Sphingobium have demonstrated to be able to degrade aromatic compounds, and thus those derived intermediates may further serve as electron donors for themselves or other microorganisms to reduce iron, nitrate and arsenate 64,65 .…”

Section: Resultsmentioning

confidence: 99%

Arsenic mobilization in a high arsenic groundwater revealed by metagenomic and Geochip analyses

Jiang

Wang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Microbial metabolisms of arsenic, iron, sulfur, nitrogen and organic matter play important roles in arsenic mobilization in aquifer. In this study, microbial community composition and functional potentials in a high arsenic groundwater were investigated using integrated techniques of RNA- and DNA-based 16S rRNA gene sequencing, metagenomic sequencing and functional gene arrays. 16S rRNA gene sequencing showed the sample was dominated by members of Proteobacteria (62.3–75.2%), such as genera of Simplicispira (5.7–6.7%), Pseudomonas (3.3–5.7%), Ferribacterium (1.6–4.4%), Solimonas (1.8–3.2%), Geobacter (0.8–2.2%) and Sediminibacterium (0.6–2.4%). Functional potential analyses indicated that organics degradation, assimilatory sulfate reduction, As-resistant pathway, iron reduction, ammonification, nitrogen fixation, denitrification and dissimilatory nitrate reduction to ammonia were prevalent. The composition and function of microbial community and reconstructed genome bins suggest that high level of arsenite in the groundwater may be attributed to arsenate release from iron oxides reductive dissolution by the iron-reducing bacteria, and subsequent arsenate reduction by ammonia-producing bacteria featuring ars operon. This study highlights the relationship between biogeochemical cycling of arsenic and nitrogen in groundwater, which potentially occur in other aquifers with high levels of ammonia and arsenic.

show abstract

Section: Resultsmentioning

confidence: 99%

Arsenic mobilization in a high arsenic groundwater revealed by metagenomic and Geochip analyses

Jiang

Wang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…While in the 5/7 microcosm, with both nitrate and nitrite as electron acceptors, Zoogloeaceae as well as Sphingomonadales (ASV3) dominated the heavy fractions, both likely representing active key populations. Some members of the Sphingomonadales are known as aerobic, but not anaerobic aromatic hydrocarbon degraders, e.g., the recently described Sphingobium aquiterrae and Sphingobium terrigena ( Revesz et al, 2018 ; Park et al, 2019 ). However, Sphingomonas mucosissima , which closely relate to ASV3 ( Figure 4 ), has not been demonstrated for toluene degradation ( Reddy and Garcia-Pichel, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Availability of Nitrite and Nitrate as Electron Acceptors Modulates Anaerobic Toluene-Degrading Communities in Aquifer Sediments

Zhu¹,

Friedrich²,

Wang³

et al. 2020

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Microorganisms are essential in the degradation of environmental pollutants. Aromatic hydrocarbons, e.g., benzene, toluene, ethylbenzene, and xylene (BTEX), are common aquifer contaminants, whose degradation in situ is often limited by the availability of electron acceptors. It is clear that different electron acceptors such as nitrate, iron, or sulfate support the activity of distinct degraders. However, this has not been demonstrated for the availability of nitrate vs. nitrite, both of which can be respired in reductive nitrogen cycling. Here via DNA-stable isotope probing, we report that nitrate and nitrite provided as electron acceptors in different concentrations and ratios not only modulated the microbial communities responsible for toluene degradation but also influenced how nitrate reduction proceeded. Zoogloeaceae members, mainly Azoarcus spp., were the key toluene degraders with nitrate-only, or both nitrate and nitrite as electron acceptors. In addition, a shift within Azoarcus degrader populations was observed on the amplicon sequence variant (ASV) level depending on electron acceptor ratios. In contrast, members of the Sphingomonadales were likely the most active toluene degraders when only nitrite was provided. Nitrate reduction did not proceed beyond nitrite in the nitrate-only treatment, while it continued when nitrite was initially also present in the microcosms. Likely, this was attributed to the fact that different microbial communities were stimulated and active in different microcosms. Together, these findings demonstrate that the availability of nitrate and nitrite can define degrader community selection and N-reduction outcomes. It also implies that nitrate usage efficiency in bioremediation could possibly be enhanced by an initial co-supply of nitrite, via modulating the active degrader communities.

show abstract

“…The role of this genus in petroleum hydrocarbon degradation is well known. Among Alphaproteobacteria (8.5% of sequence reads), the genus Sphingobium has to be noted since several members of this genus are involved in petroleum hydrocarbon degradation under aerobic conditions (Révész et al 2018). Another wellknown genus with alkane-degrading species is the Rhodococcus (class of Actinobacteria) which members were prominent representatives of the aerobic enrichment community.…”

Section: Microbial Community Compositions As Revealed By 16s Rdna Ampmentioning

confidence: 99%

Microaerobic conditions caused the overwhelming dominance of Acinetobacter spp. and the marginalization of Rhodococcus spp. in diesel fuel/crude oil mixture-amended enrichment cultures

et al. 2019

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to reveal how different microbial communities evolve in diesel fuel/crude oil-contaminated environments under aerobic and microaerobic conditions. To investigate this question, aerobic and microaerobic bacterial enrichments amended with a diesel fuel/crude oil mixture were established and analysed. The representative aerobic enrichment community was dominated by Gammaproteobacteria (64.5%) with high an abundance of Betaproteobacteriales (36.5%), followed by Alphaproteobacteria (8.7%), Actinobacteria (5.6%), and Candidatus Saccharibacteria (4.5%). The most abundant alkane monooxygenase (alkB) genotypes in this enrichment could be linked to members of the genus Rhodococcus and to a novel Gammaproteobacterium, for which we generated a high-quality draft genome using genome-resolved metagenomics of the enrichment culture. Contrarily, in the microaerobic enrichment, Gammaproteobacteria (99%) overwhelmingly dominated the microbial community with a high abundance of the genera Acinetobacter (66.3%), Pseudomonas (11%) and Acidovorax (11%). Under microaerobic conditions, the vast majority of alkB gene sequences could be linked to Pseudomonas veronii. Consequently, results shed light on the fact that the excellent aliphatic hydrocarbon degrading Rhodococcus species favour clear aerobic conditions, while oxygen-limited conditions can facilitate the high abundance of Acinetobacter species in aliphatic hydrocarbon-contaminated subsurface environments.

show abstract

Sphingobium aquiterrae sp. nov., a toluene, meta- and para-xylene-degrading bacterium isolated from petroleum hydrocarbon-contaminated groundwater

Cited by 24 publications

References 30 publications

Arsenic mobilization in a high arsenic groundwater revealed by metagenomic and Geochip analyses

Arsenic mobilization in a high arsenic groundwater revealed by metagenomic and Geochip analyses

Availability of Nitrite and Nitrate as Electron Acceptors Modulates Anaerobic Toluene-Degrading Communities in Aquifer Sediments

Microaerobic conditions caused the overwhelming dominance of Acinetobacter spp. and the marginalization of Rhodococcus spp. in diesel fuel/crude oil mixture-amended enrichment cultures

Contact Info

Product

Resources

About