Sphingobacterium solani sp. nov., isolated from potato stems

Abstract

A Gram-stain-negative, non-motile, non-spore-forming bacterium, designated MLS-26-JM13-11, was isolated from potato stems, collected in Guyuan County, Hebei Province, China. Strain MLS-26-JM13-11 could grow at 10-39 °C (optimum, 30 °C), pH 6.0-9.0 (optimum, pH 7.2) and in the presence of 0-4.0 % (w/v) NaCl (optimum, 1.0 % w/v). Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain MLS-26-JM13-11 formed a stable clade with Sphingobacterium bambusae IBFC2009 and Sphingobacterium griseoflavum SCU-B140, with the 16S rRNA gene sequence similarities ranging from 95.9 % to 97.0 %. The major cellular fatty acids comprised iso-C15 : 0 (36.9 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c, 34.0 %), C16 : 0 (3.0 %) and iso-C17 : 0 3-OH (13.4 %). Strain MLS-26-JM13-11 contained sphingoglycolipid, phosphatidyl ethanolamine, six unknown lipids, one unknown aminolipid, four unknown polarlipids and two unknown aminophospholipids. The isoprenoid quinone was MK-7. The DNA G+C content was 42.6 mol%. Furthermore, the average nucleotide identity and in silico estimated DNA-DNA reassociation values among MLS-26-JM13-11 and S. bambusae KCTC 22814 were in all cases below the respective threshold for species differentiation. On the basis of phenotypic, genotypic and phylogenetic evidence, strain MLS-26-JM13-11 (=ACCC 60057=JCM 32274) represents a novel species within the genus Sphingobacterium, for which the name Sphingobacterium solani sp. nov. is proposed.

Sphingobacterium oryzagri sp. nov., isolated from rice paddy soil

“…S3). The results of the polar lipid and isoprenoid quinone analysis obtained from the present study were consistent with the patterns observed in members of the genus Sphingobacterium [3–10].…”

“…Briefly, the major protein groups included translation, ribosomal structure and biogenesis (J), transcription (K), signal transduction mechanisms (T), cell wall/membrane/envelope biogenesis (M), carbohydrate transport and metabolism (G), and inorganic ion transport and metabolism (P). While strain M6-31 T and six phylogenetic neighbours possessed cell motility-related genes (N), no genes were observed to be involved in flagella, aligning with the morphology of the genus Sphingobacterium, characterized by the absence of a flagellum [3–10]. A dbCAN2 search revealed candidate genes for carbohydrate-active enzyme families, including glycoside hydrolases (GH), glycosyltransferases (GT), carbohydrate-binding module (CBM), carbohydrate esterases (CE), polysaccharide lyases (PL) and auxiliary activities (AA) across all seven genomes, while the subfamily GH30_4, GH30_5, GH30_6, GH35 and GT2_glyco_transf_2_5 existing only in strain M6-31 T (Table S3).…”

“…At the time of writing, the genus represented by the type species Sphingobacterium spiritivorum, comprised 68 validly published species (https://lpsn.dsmz.de/). Sphingobacterium species have been discovered from diverse environments, including aquaculture water [3], food-waste compost [4], potato stem [5], raw milk [6], rhizosphere [7] and soil [8][9][10]. Members of the genus are characterized by aerobic, Gram-stain-negative, rod-shaped, nonspore-forming and yellow-pigmented bacteria, containing high concentrations of sphingophospholipids in their cells.…”

Sphingobacterium micropteri sp. nov. and Sphingobacterium litopenaei sp. nov., isolated from aquaculture water

“…Bacteria of this genus are characterized by a high concentration of sphingophospholipids and are Gram-stain-negative, non-motile, non-spore-forming, positive for catalase and oxidase, but negative for gelatinase, heparinase and indole production, and have iso-C 15 : 0 , iso-C 15 : 0 2-OH, C 16 : 1 ω7c and C 17 : 0 3-OH as the main fatty acids [3]. Members of the genus Sphingobacterium are widely distributed in various natural environments such as soil, desert, compost, sludge, plant, animal and clinical samples [4][5][6][7][8][9]. Some Sphingobacterium strains are capable of degrading pollutants such as polycyclic aromatic hydrocarbons, pesticides, herbicides and antibiotics [7,[10][11][12].…”

Sphingobacterium lumbrici sp. nov., a novel bacterium isolated from wormcast of Eisenia foetida

“…DNA G+C content ranges from 37.3 to 44.5 mol% [4]. These species have been isolated from various environments including raw milk, plants, diverse soil habitats, faeces, activated sludge, clinical specimens, freshwater habitats and nodule surface of soybean and include Sphingobacterium terrae [4], Sphingobacterium bovisgrunnientis [5], Sphingobacterium tabacisoli [6], Sphingobacterium solani [7], Sphingobacterium composti [8], Sphingobacterium cellulitidis [9], Sphingobacterium yanglingense [10], Sphingobacterium suaedae [11], Sphingobacterium caeni [12] and Sphingobacterium corticibacter [13]. Recently, Sphingobacterium olei [14], Sphingobacterium chungjuense [15] and Sphingobacterium cavernae [16] were reported.…”