The taxonomic status of a bacterium, strain DC-8 T , isolated from activated sludge, was determined using a polyphasic taxonomic approach. The cells of strain DC-8 T were Gramnegative, non-motile, non-spore-forming and rod-shaped. The isolate grew at temperature range of 10-40 6C (optimum 30-35 6C), pH range of 5.0-10.0 (optimum 6.5-8.0) and NaCl concentrations of 0-5 % (optimum 0-1 %). The predominant menaquinone of strain DC-8 T was MK-7 and major fatty acids were summed feature 3 (C 16 : 1 v6c and/or C 16 : 1 v7c; 39.7 %), iso-C 15 : 0 (33.7 %) and C 16 : 0 (5.2 %). The DNA G+C content was 39.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain DC-8 T was a member of the genus Sphingobacterium. Strain DC-8 T shared the highest similarity with Sphingobacterium siyangense SY1 T (98.4 %), Sphingobacterium multivorum IAM 14316 T (98.3 %), Sphingobacterium canadense CR11 T (98.0 %) and Sphingobacterium detergens 6.2S T (97.9 %) and shared less than 97 % similarity with other members of the genus Sphingobacterium. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness values between strain DC-8 T and its closest phylogenetic neighbours were below 70 %. Based on the phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics, strain DC-8 T was clearly distinguished from all recognized species of the genus Sphingobacterium and should be classified as a representative of a novel species of the genus Sphingobacterium, for which the name Sphingobacterium caeni sp. nov. is proposed. The type strain is DC-8 T (5CCTCC AB 2012020 T 5KACC 16850 T ).The genus Sphingobacterium was proposed by Yabuuchi et al. (1983) to include Gram-negative rods that are positive for catalase and oxidase, negative for heparinase, gelatinase and indole production and contain iso-C 15 : 0 , iso-C 15 : 0 2-OH, C 16 : 1 v7c and C 17 : 0 3-OH as the main fatty acids (Takeuchi & Yokota, 1992; Steyn et al., 1998). Members of the genus Sphingobacterium are metabolically versatile and are widely distributed in soil (Shivaji et al., 1992;Mehnaz et al., 2007;Liu et al., 2008;Wei et al., 2008; Matsuyama et al., 2008; Duan et al., 2009; He et al., 2010;Choi & Lee, 2012; Marqués et al., 2012), raw milk (Schmidt et al., 2012), fresh leaves (Liu et al., 2012), clinical specimens (Yabuuchi et al., 1983), activated sludge (Zhang et al., 2012) and compost (Ten et al., 2006; Kim et al., 2006; Yoo et al., 2007). During an investigation of the microbial diversity of wastewater from the wastewater treatment facility for butachlor (N-butoxymethl-chloro-2,6-diethylacetnilide) manufacture (Kunshan, Jiangsu province, China, 31 u 229 N 120 u 569 E), a bacterium, designated strain DC-8 T , was isolated from the activated sludge. Based on the results of a polyphasic taxonomic study, this strain is considered to represent a novel species of the genus Sphingobacterium.For investigation of morphological features, strain DC-8 T was cultivated aerobically on TSA (D...