Spherowell, a novel system inducing autonomous spheroid formation of cell monolayers

Fürsatz, Marian; Gerges, P.; Wolbank, Susanne; Nürnberger, Sylvia

doi:10.1016/j.joca.2021.02.530

Cited by 1 publication

(1 citation statement)

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“…In addition to the above methods, another productive way of generating spheroid/organoids with similar diameters is by rolling up monolayer cell cultures via compartmentation of cell culture surfaces utilising laser engraving (grid plates) [57]. Cells are cultured as monolayers inside the dish, subdivided by compartments; the plate then triggers the retraction at the edge of each compartment and therefore rolls the monolayer up as a spheroid [58]. Typically, about a hundred spheroids/ organoids can be made from one 60 mm dish.…”

Section: Self-assembly Culturesmentioning

confidence: 99%

Progress of 3D Organoid Technology for Preclinical Investigations: Towards Human In Vitro Models

Liu

Xu²,

Abraham³

et al. 2022

IJDDP

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Review Progress of 3D Organoid Technology for Preclinical Investigations: Towards Human In Vitro Models Yingjuan Liu *, Honglin Xu, Sabu Abraham, Xin Wang, and Bernard D. Keavney* Division of Cardiovascular Sciences, Faculty of Biology, Medicine and Health, University of Manchester, M13 9PT, UK. * Correspondence: yingjuan.liu@manchester.ac.uk (Yingjuan Liu); bernard.keavney@manchester.ac.uk (Bernard D. Keavney) Received: 1 November 2022 Accepted: 24 November 2022 Published: 21 December 2022 Abstract: Currently, with an increased requirement for new therapeutic strategies, preclinical drug testing or screening platforms have rapidly evolved in recent years. In comparison to traditional 2D cell cultures, 3D organoids or spheroids with or without scaffolds improve the microenvironment of in vitro cultures, advancing the in vitro biological observation and enabling mechanistic studies of drug reactions in the human tissue-like environment. 3D organoids and spheroids are straightforward to produce, and relatively uniform in size and shape. This helps to facilitate high throughput screening requirements. Spheroids and organoids have been applied in anti-cancer drug testing, toxicity evaluations, as well as mechanism studies for variable organ systems, including the intestine, liver, pancreas, brain, and heart. Among 3D cultures of spheroids and organoids, ‘tumour spheroids’ formed by dissociated tumour tissues or cancer cell lines are relatively simple in composition and commonly applied to anticancer drug screening. The ‘healthy organoids’ differentiated from hiPSCs/hESCs are more complex in cell composition, distribution, structure and function with higher similarity to in vivo organs, and have found applications in toxicity tests, personalised medicine, and therapeutic and mechanistic studies. In most cases, the multicellular 3D organoids are more resistant and stable in reaction to stimulations or chemicals in vitro , suggesting more accurate modelling of in vivo responses. Here, we review recent progress in human-origin organoid/spheroid systems and their applications in preclinical studies.

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Section: Self-assembly Culturesmentioning

confidence: 99%