Spheroid Coculture of Human Gingiva-Derived Progenitor Cells With Endothelial Cells in Modified Platelet Lysate Hydrogels

Abstract

Cell coculture strategies can promote angiogenesis within tissue engineering constructs. This study aimed to test the angiogenic potential of human umbilical vein endothelial cells (HUVEC) cocultured with gingiva-derived progenitor cells (GPC) as spheroids in a xeno-free environment. Human platelet lysate (HPL) was used as a cell culture supplement and as a hydrogel matrix (HPLG) for spheroid encapsulation. HUVEC and HUVEC + GPC (1:1 or 5:1) spheroids were encapsulated in various HPLG formulations. Angiogenesis was assessed via in vitro sprouting and in vivo chick chorioallantoic membrane (CAM) assays. HUVEC revealed characteristic in vitro sprouting in HPL/HPLG and this was significantly enhanced in cocultures with GPC (p < 0.05). A trend for greater sprouting was observed in 5:1 vs 1:1 HUVEC + GPC spheroids and in certain HPLG formulations (p > 0.05). Both HUVEC and HUVEC + GPC spheroids in HPLG revealed abundant and comparable neoangiogenesis in the CAM assay (p > 0.05). Spheroid coculture of HUVEC + GPC in HPLG represents a promising strategy to promote angiogenesis.

Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells

“…ADSCs cultured on HPL-supplemented PEG hydrogel showed high speed migration, covered long distances, and migrated only in the direction of the HPL-loaded PEG hydrogel [ 40 ]. In addition, In the in vitro co-culture system, HPL supplemented medium promoted the angiogenesis of gingival mesenchymal stem cells [ 41 ]. HPL hydrogel stimulates pro-angiogenic activity by promoting the growth and invasion of human MSCs in a 3D environment and enhancing endothelial cell sprouting in vitro.…”

Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis

“…MSCs demonstrated increased invasion in the PL gel compared to fibrin hydrogels [ 74 ]. In the recent study of Shanbhag et al [ 98 ], heterotypic spheroids containing HUVECs and gingiva-derived progenitor cells (GPCs) were cultured in pure PL gel or PL gel that was supplemented with different concentrations of exogenous fibrinogen to improve rheological properties. After 72 h, the sprouting was comparable in pure PL gel and PL gel with 1.25 mg/mL fibrinogen, while in PL gel that was supplemented with 2.5 mg/mL, it was substantially enhanced.…”

“…After 72 h, the sprouting was comparable in pure PL gel and PL gel with 1.25 mg/mL fibrinogen, while in PL gel that was supplemented with 2.5 mg/mL, it was substantially enhanced. It is worth mentioning that the higher concentrations of fibrinogen (>2.5 mg/mL) decreased both the viability of HUVECs and sprouting [ 98 ].…”

“…The earliest work listed in Table 1 was published in 2001 [ 16 ] and also the latest papers were published in 2021 [ 46 , 98 ]. Several tissue sources were utilized to obtain ECs.…”

Efficiency of accelerated electron beam sterilization of a hydrogel for 3D cultivation of mesenchymal multipotent cells

“…Более того, ГГ, дополненный ростовыми факторами, может значительно улучшить пролиферацию клеток в системе 3D-культивирования и обеспечить управляемую миграцию ММСК в целевую область [7]. 3D-культивирование ММСК способствует структурообразованию клеточной культуры и улучшает локальную перфузию тканей, что подтверждает перспективность использования ГГ в качестве скаффолда для локальной доставки MМCК и стимуляции ангиогенеза [8].…”